Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene,REC102

Yeast ◽  
2002 ◽  
Vol 19 (2) ◽  
pp. 99-114 ◽  
Author(s):  
Kai Jiao ◽  
John J. Nau ◽  
Marc Cool ◽  
William M. Gray ◽  
Jan S. Fassler ◽  
...  
Keyword(s):  
Meiotic Recombination ◽  
Phylogenetic Footprinting ◽  
Regulatory Elements ◽  
Recombination Gene

Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast

1993 ◽  
Vol 23 (4) ◽  
pp. 295-304 ◽  
Author(s):  
Doug Pittman ◽  
Wei Lu ◽  
Robert E. Malone
Keyword(s):  
Molecular Analysis ◽  
Meiotic Recombination ◽  
Recombination Gene

Immunoglobulin molecular genetics: the prospects for mutational analysis of the chromosomal immunoglobulin genes

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 175-181 ◽  
Author(s):  
Marc J. Shulman ◽  
Lucine Bosnoyan ◽  
Catherine Collins ◽  
Nancy Pennell ◽  
Mark D. Baker
Keyword(s):  
Gene Expression ◽  
Homologous Recombination ◽  
Mutational Analysis ◽  
Regulatory Elements ◽  
Hybridoma Cells ◽  
Immunoglobulin Genes ◽  
Chromosomal Dna ◽  
Cis Acting ◽  
Chromosomal Genes ◽  
Recombination Gene

Homologous recombination between transferred and chromosomal DNA can be used to effect precise, predetermined modifications of the chromosomal genes. Ultimately this phenomenon should allow the assessment of genetic regulatory elements as they function in the normal chromosomal environment. We have previously described a system for isolating mutant hybridoma cells that are defective in immunoglobulin (Ig) production, with a view toward using these mutants to define cis-acting elements that influence Ig gene expression. Here we describe results that indicate that homologous recombination between transferred and chromosomal Ig genes can be used to map Ig mutations by marker rescue.Key words: homologous recombination, gene expression.

scholarly journals Dmc1 is a candidate for temperature tolerance during wheat meiosis

2019 ◽  
Author(s):  
Tracie Draeger ◽  
Azahara Martin ◽  
Abdul Kader Alabdullah ◽  
Ali Pendle ◽  
María-Dolores Rey ◽  
...  
Keyword(s):  
High Temperatures ◽  
Chromosome Pairing ◽  
Meiotic Recombination ◽  
Low Temperatures ◽  
Chinese Spring ◽  
Deletion Mutant ◽  
Meiotic Chromosome ◽  
Wheat Chromosome ◽  
Chromosome Synapsis ◽  
Recombination Gene

AbstractWe have assessed the effects of high and low temperatures on meiotic chromosome synapsis and crossover formation in the hexaploid wheat (Triticum aestivum L.) variety ‘Chinese Spring’. At low temperatures, asynapsis and chromosome univalence have been observed before in Chinese Spring lines lacking the long arm of chromosome 5D (5DL), which led to the proposal that 5DL carries a gene (Ltp1) that stabilises wheat chromosome pairing at low temperatures. In the current study, Chinese Spring wild type and 5DL interstitial deletion mutant plants were exposed to low (13°C) or high (30°C) temperatures in controlled environment rooms during a period from premeiotic interphase to early meiosis I. A 5DL deletion mutant was identified whose meiotic chromosomes exhibit extremely high levels of asynapsis and chromosome univalence at metaphase I after seven days at 13°C. This suggests that the mutant, which we name ttmei1 (temperature tolerance in meiosis 1) has a deletion of a gene that, like Ltp1, normally stabilises chromosome pairing at low temperatures. Immunolocalisation of the meiotic proteins ASY1 and ZYP1 on ttmei1 mutants showed that low temperature results in a failure to complete synapsis at pachytene. After 24 hours at 30°C, ttmei1 mutants exhibited a reduced number of crossovers and increased univalence, but to a lesser extent than at 13°C. KASP genotyping revealed that ttmei1 has a 4 Mb deletion in 5DL. Of 41 genes within this deletion region, the strongest candidate for the stabilisation of chromosome pairing at low (and possibly high) temperatures is the meiotic recombination gene Dmc1.Key messageThe meiotic recombination gene Dmc1 on wheat chromosome 5D has been identified as a candidate for the maintenance of normal chromosome synapsis and crossover at low and possibly high temperatures.

Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.

Genetics ◽  
1992 ◽  
Vol 132 (1) ◽  
pp. 113-123 ◽  
Author(s):  
P Detloff ◽  
M A White ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mismatch Repair ◽  
Meiotic Recombination ◽  
Present Evidence ◽  
Homologous Chromosomes ◽  
A Site ◽  
His4 Gene ◽  
Heteroduplex Formation ◽  
Recombination Gene

Abstract Heteroduplexes formed between genes on homologous chromosomes are intermediates in meiotic recombination. In the HIS4 gene of Saccharomyces cerevisiae, most mutant alleles at the 5' end of the gene have a higher rate of meiotic recombination (gene conversion) than mutant alleles at the 3' end of the gene. Such gradients are usually interpreted as indicating a higher frequency of heteroduplex formation at the high conversion end of the gene. We present evidence indicating that the gradient of conversion at HIS4 primarily reflects the direction of mismatch repair rather than the frequency of heteroduplex formation. We also identify a site located between the 5' end of HIS4 and the 3' end of BIK1 that stimulates heteroduplex formation at HIS4 and BIK1.

scholarly journals Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe

2005 ◽  
Vol 48 (5) ◽  
pp. 323-333 ◽  
Author(s):  
Eveline Doll ◽  
Monika Molnar ◽  
Yasushi Hiraoka ◽  
Jürg Kohli
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Recombination Gene

scholarly journals Five RecA-like Proteins of Schizosaccharomyces pombe Are Involved in Meiotic Recombination

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1031-1043
Author(s):  
A L Grishchuk ◽  
J Kohli
Keyword(s):  
Escherichia Coli ◽  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Sequence Similarity ◽  
Intragenic Recombination ◽  
Wild Type ◽  
Spore Viability ◽  
Viability Analysis ◽  
Recombination Protein ◽  
Recombination Gene

Abstract The genome of Schizosaccharomyces pombe contains five genes that code for proteins with sequence similarity to the Escherichia coli recombination protein RecA: rad51+, rhp55+, rhp57+, rlp1+, and dmc1+. We analyzed the effect of deletion of each of these genes on meiotic recombination and viability of spores. Meiotic recombination levels were different from wild type in all recA-related mutants in several genetic intervals, suggesting that all five RecA homologs of S. pombe are required for normal levels of meiotic recombination. Spore viability was reduced in rad51, rhp55, and rhp57 mutants, but not in rlp1 and dmc1. It is argued that reduction of crossover is not the only cause for the observed reduction of spore viability. Analysis of double and triple mutants revealed that Rad51 and Dmc1 play major and partially overlapping roles in meiotic recombination, while Rhp55, Rhp57, and Rlp1 play accessory roles. Remarkably, deletion of Rlp1 decreases the frequency of intergenic recombination (crossovers), but increases intragenic recombination (gene conversion). On the basis of our results, we present a model for the involvement of five RecA-like proteins of S. pombe in meiotic recombination and discuss their respective roles.

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