Confocal surface plasmon microscopy with vortex beam illumination for biosensing application: Label free biosensing application of confocal surface plasmon microscope

2015 ◽  
Author(s):  
Suejit Pechprasarn ◽  
Wai-Kin Chow ◽  
Jingkai Meng ◽  
Michael G Somekh
Keyword(s):  
Surface Plasmon ◽  
Vortex Beam ◽  
Label Free ◽  
Surface Plasmon Microscopy

Surface plasmon microscopy by spatial light switching for label-free imaging with enhanced resolution

2018 ◽  
Vol 43 (4) ◽  
pp. 959 ◽  
Author(s):  
Taehwang Son ◽  
Changhun Lee ◽  
Jinwon Seo ◽  
In-Hong Choi ◽  
Donghyun Kim
Keyword(s):  
Surface Plasmon ◽  
Label Free ◽  
Enhanced Resolution ◽  
Surface Plasmon Microscopy

Enhanced surface plasmon microscopy based on multi-channel spatial light switching for label-free neuronal imaging

2019 ◽  
Vol 146 ◽  
pp. 111738
Author(s):  
Taehwang Son ◽  
Changhun Lee ◽  
Gwiyeong Moon ◽  
Dongsu Lee ◽  
Eunji Cheong ◽  
...  
Keyword(s):  
Surface Plasmon ◽  
Label Free ◽  
Neuronal Imaging ◽  
Surface Plasmon Microscopy ◽  
Enhanced Surface

Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Localized Surface Plasmon ◽  
Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

scholarly journals Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application

2021 ◽  
Vol 11 (3) ◽  
pp. 1002
Author(s):  
Xue Wang ◽  
Xinchao Lu ◽  
Chengjun Huang
Keyword(s):  
Laser Scanning ◽  
Second Harmonic ◽  
Biological Tissues ◽  
Biological Imaging ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Coherent Raman ◽  
Surface Plasmon Microscopy

By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scattering microscopy, second harmonic generation microscopy and scanning localized surface plasmon microscopy are discussed with their fundamentals, features and recent progress. The applications of label-free biological imaging of these laser scanning microscopies are also introduced. Finally, the performance of the microscopies is compared and the limitation and perspectives are summarized.

scholarly journals A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5784-5791 ◽  
Author(s):  
Qiang Su ◽  
Gilbert Nöll
Keyword(s):  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Molecular Beacons ◽  
Label Free ◽  
Background Fluorescence ◽  
Detection Strategy ◽  
Cutting Surface ◽  
Label Free Detection ◽  
Lower Background

Cutting surface-bound optical molecular beacons results in a sandwich-like detection strategy with lower background fluorescence.

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