Data mining methods of lung cancer diagnosis by saliva tests using surface enhanced Raman spectroscopy

2014 ◽  
Author(s):  
Wenyan Liu ◽  
Ziyan Man ◽  
Lin Hua ◽  
Anyu Chen ◽  
Yan Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Data Mining ◽  
Raman Spectroscopy ◽  
Cancer Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lung Cancer Diagnosis ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Mining Methods

scholarly journals Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Xiaowei Cao ◽  
Zhenyu Wang ◽  
Liyan Bi ◽  
Jie Zheng
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cell Lung Cancer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Small Cell ◽  
Label Free ◽  
Small Cell Lung ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a good candidate for the development of fast and easy-to-use diagnostic tools, possibly used on serum in screening tests. In this study, a potential label-free serum test based on SERS spectroscopy was developed to analyze human serum for the diagnosis of the non-small cell lung cancer (NSCLC). We firstly synthesized novel highly branched gold nanoparticles (HGNPs) at high yield through a one-step reduction of HAuCl4 with dopamine hydrochloride at 60°C. Then, HGNP substrates with good reproducibility, uniformity, and high SERS effect were fabricated by the electrostatically assisted (3-aminopropyl) triethoxysilane-(APTES-) functionalized silicon wafer surface-sedimentary self-assembly method. Using as-prepared HGNP substrates as a high-performance sensing platform, SERS spectral data of serum obtained from healthy subjects, lung adenocarcinoma patients, lung squamous carcinoma patients, and large cell lung cancer patients were collected. The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. SERS spectra were analyzed by principal component analysis (PCA), which was able to distinguish different types of NSCLC. Considering its time efficiency, being label-free, and sensitivity, SERS based on HGNP substrates is very promising for mass screening NSCLC and plays an important role in the detection and prevention of other diseases.

Combination assay of lung cancer associated serum markers using surface-enhanced Raman spectroscopy

2016 ◽  
Vol 4 (10) ◽  
pp. 1811-1817 ◽  
Author(s):  
Chunyuan Song ◽  
Yanjun Yang ◽  
Boyue Yang ◽  
Linghua Min ◽  
Lianhui Wang
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Human Serum ◽  
Surface Enhanced Raman Spectroscopy ◽  
Serum Markers ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A highly sensitive and specific SERS-based combination assay of lung cancer associated serum markers is reported, and the LODs of CEA and NSE in human serum specimens are 1.48 pg mL−1 and 2.04 pg mL−1, respectively.

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