Quantum dots-based lateral flow strip assay for rapid detection of clenbuterol

2011 ◽  
Author(s):  
Wei Luo ◽  
Huaiming Li ◽  
Hengyi Xu ◽  
Yonghua Xiong ◽  
Hua Wei ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip

Rapid detection of Shiga toxin type II using lateral flow immunochromatography test strips of colorimetry and fluorimetry

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Tian Lu ◽  
Kai-Di Zhu ◽  
Chao Huang ◽  
Tian Wen ◽  
Yong-Jun Jiao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Shiga Toxin ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Cdte Quantum Dots ◽  
Type Ii ◽  
Immunochromatographic Test ◽  
Test Strips

Two types of lateral flow immunochromatographic test strips (LFITS) using gold nanoparticles and fluorescent CdTe quantum dots (QDs) as signal labels, respectively, were developed for Shiga toxin type II (STX2) assays.

scholarly journals Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

2012 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
Fabio Cimaglia ◽  
Alessandro Aliverti ◽  
Maurizio Chiesa ◽  
Palmiro Poltronieri ◽  
Enrico De Lorenzis ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Detection System ◽  
Lateral Flow ◽  
Fluorescence Signal ◽  
Human Pathogens ◽  
Detection Zone ◽  
Diagnostic Investigation

A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (<em>fprA</em>). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated <em>fprA</em> domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of <em>fprA</em> protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of <em>fprA</em> protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of <em>Mycobacterium tuberculosis</em> and other human pathogens in clinical specimens.

scholarly journals Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers

2018 ◽  
Vol 13 (1) ◽  
Author(s):  
Jie Li ◽  
Le Jing ◽  
Yuzhu Song ◽  
Jinyang Zhang ◽  
Qiang Chen ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip

scholarly journals Development of an immunochromatographic lateral flow strip test for the rapid detection of diclofenac in medicinal wine

2020 ◽  
Vol 31 (1) ◽  
pp. 205-216 ◽  
Author(s):  
Xingdong Yang ◽  
Yinbiao Wang ◽  
Jifei Yang ◽  
Zhongke Sun ◽  
Cuiwei Chu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Strip Test

scholarly journals Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces

2021 ◽  
Vol 59 (2) ◽  
pp. 167-171
Author(s):  
Yao-Dong Wu ◽  
Qi-Qi Wang ◽  
Meng Wang ◽  
Hany M. Elsheikha ◽  
Xin Yang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Haemonchus Contortus ◽  
Rapid Detection ◽  
Small Ruminants ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Lateral Flow Strip ◽  
Specific Sequence ◽  
Amplification Assay ◽  
Recombinase Polymerase Amplification Assay

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of <i>Haemonchus contortus</i> in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected <i>H. contortus</i> DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of <i>H. contortus</i> and merits further evaluation.

scholarly journals Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248536
Author(s):  
Sitthichai Kanokudom ◽  
Thachaporn Assawakongkarat ◽  
Yukihiro Akeda ◽  
Panan Ratthawongjirakul ◽  
Rungtip Chuanchuen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Nucleotide Sequencing ◽  
Pork Meat ◽  
Lateral Flow Strip ◽  
Extended Spectrum

The emergence and dissemination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is a global health issue. Food-producing animals, including pigs, are significant reservoirs of antimicrobial resistance (AMR), which can be transmitted to humans. Thus, the rapid detection of ESBLs is required for efficient epidemiological control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was established for the rapid and simultaneous detection of multiple bla genes in a single reaction. Visible blue lines, indicating the presence of the blaCTX-M, blaSHV, and blaOXA genes, were observed within 10 min by the naked eye. The limit of detection of all three genes was 2.5 ng/25 μL, and no cross-reactivity with seven commensal aerobic bacteria was observed. A total of 93.9% (92/98) and 96% (48/50) of the E. coli isolates from pork meat and fecal samples, respectively, expressed an ESBL-producing phenotype. Nucleotide sequencing of the PCR amplicons showed that blaCTX-M was the most prevalent type (91.3–95.83%), of which the main form was blaCTX-M-55. The sensitivity and specificity of the RPA-LFA were 99.2% and 100%, respectively, and were in almost perfect agreement (κ = 0.949–1.000) with the results from PCR sequencing. Thus, the RPA-LFA is a promising tool for rapid and equipment-free ESBL detection and may facilitate clinical diagnosis in human and veterinary medicine, as well as AMR monitoring and surveillance.

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