Rapid detection of Shiga toxin type II using lateral flow immunochromatography test strips of colorimetry and fluorimetry

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Tian Lu ◽  
Kai-Di Zhu ◽  
Chao Huang ◽  
Tian Wen ◽  
Yong-Jun Jiao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Shiga Toxin ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Cdte Quantum Dots ◽  
Type Ii ◽  
Immunochromatographic Test ◽  
Test Strips

Two types of lateral flow immunochromatographic test strips (LFITS) using gold nanoparticles and fluorescent CdTe quantum dots (QDs) as signal labels, respectively, were developed for Shiga toxin type II (STX2) assays.

scholarly journals Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

2012 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
Fabio Cimaglia ◽  
Alessandro Aliverti ◽  
Maurizio Chiesa ◽  
Palmiro Poltronieri ◽  
Enrico De Lorenzis ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Detection System ◽  
Lateral Flow ◽  
Fluorescence Signal ◽  
Human Pathogens ◽  
Detection Zone ◽  
Diagnostic Investigation

A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (<em>fprA</em>). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated <em>fprA</em> domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of <em>fprA</em> protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of <em>fprA</em> protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of <em>Mycobacterium tuberculosis</em> and other human pathogens in clinical specimens.

A novel enhancement assay for immunochromatographic test strips using gold nanoparticles

2006 ◽  
Vol 385 (8) ◽  
pp. 1414-1420 ◽  
Author(s):  
Ryo Tanaka ◽  
Teruko Yuhi ◽  
Naoki Nagatani ◽  
Tatsuro Endo ◽  
Kagan Kerman ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Immunochromatographic Test ◽  
Test Strips

scholarly journals Theoretical studies of excitons in type II CdSe/CdTe quantum dots

2014 ◽  
Vol 526 ◽  
pp. 012005 ◽  
Author(s):  
Jacek M Miloszewski ◽  
Stanko Tomić ◽  
David Binks
Keyword(s):  
Quantum Dots ◽  
Cdte Quantum Dots ◽  
Theoretical Studies ◽  
Type Ii

A thin CdSe shell boosts the electron transfer from CdTe quantum dots to methylene blue

Nanoscale ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 2162-2169 ◽  
Author(s):  
L. Dworak ◽  
S. Roth ◽  
M. P. Scheffer ◽  
A. S. Frangakis ◽  
J. Wachtveitl
Keyword(s):  
Methylene Blue ◽  
Quantum Dots ◽  
Electron Transfer ◽  
Charge Transfer ◽  
Cdte Quantum Dots ◽  
Type Ii ◽  
Cdte Nanocrystals ◽  
Transfer Properties

The CdSe coating of CdTe nanocrystals produces quasi type-II quantum dots with improved charge transfer properties.

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