BurstMem: A high-performance burst buffer system for scientific applications

2014 ◽  
Author(s):  
Teng Wang ◽  
Sarp Oral ◽  
Yandong Wang ◽  
Brad Settlemyer ◽  
Scott Atchley ◽  
...  
Keyword(s):  
High Performance ◽  
Buffer System ◽  
Scientific Applications

Bioanalytical method development and validation of simultaneous analysis of telmisartan and hydrochlorthiazide in human plasma using LC-MS/MS

2016 ◽  
Vol 5 (03) ◽  
pp. 4862 ◽  
Author(s):  
Mathew George* ◽  
Lincy Joseph ◽  
Arpit Kumar Jain ◽  
Anju V.
Keyword(s):  
Human Plasma ◽  
Retention Time ◽  
High Performance ◽  
Method Development ◽  
Matrix Effects ◽  
Solid Phase ◽  
Internal Standard ◽  
Assay Method ◽  
Buffer System ◽  
Internal Standards

A simple, sensitive, rapid and economic high performance thin layer chromatographic method and a mass spectroscopic assay method has been developed for the quantification of telmisartan and hydrochlorthiazide combination in human plasma. The internal standards and analytes were extracted from human plasma by solid-phase extraction with HLB Oasis1cc (30mg) catridges. The scanning and optimization for the samples are done using methanol: water (50:50). The samples were chromatographed using reverse phase chromatography with C-18 column of different manufacturers like Ascentis C18 (150×4. 6, 5µ) using the buffer system Acetonitrile: Buffer (80:20%v/v) which consist of 2±0. 1Mm ammonium format at a flow rate of 0. 7ml/min at a column oven temperature 35±10c. The internal standard used was hydrochlorthiazide13c1, d2 and telmisartand3. The extraction techniques include conditioning, loading, washing and elution, drying followed by reconstitution of the dried samples. The volume injected was 10µl with the retention time of 3-4 min for telmisartan, 1-2 min for hydrochlorthiazide and for the internal standards the retention time was 3-4 min for telmisartand3 and 1-2 min for hydrochlorthiazide c13d2. The rinsing solution was Acetonitrile: HPLC grade water in the ratio (50:50). The above developed method was validated using various parameters like selectivity and sensitivity, accuracy and precision, matrix effects, % recovery and various stability studies. The method was proved to be sensitive, accurate, precise and reproducible. The preparation showed high recovery for the quantitative determination of telmisartan and hydrochlorthiazide in human plasma.

Topic 15: High Performance and Scientific Applications

2012 ◽  
pp. 779-780
Author(s):  
Thomas Ludwig ◽  
Costas Bekas ◽  
Alice Koniges ◽  
Kengo Nakajima
Keyword(s):  
High Performance ◽  
Scientific Applications

A Liquid-Chromatographic Analysis for Indomethacin in Serum

1979 ◽  
Vol 25 (4) ◽  
pp. 589-591 ◽  
Author(s):  
Steven J Soldin ◽  
Terese Gero
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Internal Standard ◽  
Ethylene Dichloride ◽  
Buffer System ◽  
Potassium Phosphate Buffer ◽  
Column Temperature ◽  
Analytical Recovery ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a "high-performance" liquid-chromato-graphic assay for measuring indomethacin in 100 µL of serum. The procedure involves an ethylene dichloride extraction of indomethacin and internal standard (2,5-diphenyloxazole) from serum. The organic phase is separated and evaporated, and the residue dissolved in a 40/60 (by vol) mixture of potassium phosphate buffer (10 mmol/L, pH 3.0) and acetonitrile. An aliquot of this solution is injected into the chromatograph. We used a µ-Bondapak C-18 column with the above-mentioned buffer system as mobile phase, a flow rate of 3.0 mL/min, and a column temperature of 40 °C. Indomethacin and the internal standard are detected by their absorbance at 200 nm and quantitated by measuring peak heights. The procedure allows for the reliable analysis for indomethacin in serum at concentrations > 100 µg/L. Analytical recovery for indomethacin was 66-68%, and the method affords good day-to-day precision (CV <11 %).

scholarly journals High-performance capillary electrophoresis of core histones and their acetylated modified derivatives

1992 ◽  
Vol 283 (2) ◽  
pp. 467-471 ◽  
Author(s):  
H Lindner ◽  
W Helliger ◽  
A Dirschlmayer ◽  
M Jaquemar ◽  
B Puschendorf
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Rat Liver ◽  
Phosphate Buffer ◽  
High Performance ◽  
Buffer System ◽  
Zone Electrophoresis ◽  
Core Histones ◽  
Capillary Zone ◽  
High Performance Capillary Electrophoresis

By using high-performance capillary electrophoresis, we have successfully separated rat liver core histones into several subfractions. Inconvenient interactions of the highly basic proteins with the capillary wall were eliminated by a phosphate buffer system containing 0.03% hydroxyprophylmethylcellulose. Sample amounts of a few nanolitres were analysed within about 20 min. Multiacetylated histones H4 and H3 from induced Friend erythroleukaemic cells prepurified by h.p.l.c. were clearly separated into their non-acetylated and distinct acetylated forms. Our results illustrate that the application of capillary zone electrophoresis on its own or in combination with h.p.l.c. to the analysis of histones provides an important new alternative to traditional gel electrophoreses.

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