scholarly journals High-performance capillary electrophoresis of core histones and their acetylated modified derivatives

1992 ◽  
Vol 283 (2) ◽  
pp. 467-471 ◽  
Author(s):  
H Lindner ◽  
W Helliger ◽  
A Dirschlmayer ◽  
M Jaquemar ◽  
B Puschendorf
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Rat Liver ◽  
Phosphate Buffer ◽  
High Performance ◽  
Buffer System ◽  
Zone Electrophoresis ◽  
Core Histones ◽  
Capillary Zone ◽  
High Performance Capillary Electrophoresis

By using high-performance capillary electrophoresis, we have successfully separated rat liver core histones into several subfractions. Inconvenient interactions of the highly basic proteins with the capillary wall were eliminated by a phosphate buffer system containing 0.03% hydroxyprophylmethylcellulose. Sample amounts of a few nanolitres were analysed within about 20 min. Multiacetylated histones H4 and H3 from induced Friend erythroleukaemic cells prepurified by h.p.l.c. were clearly separated into their non-acetylated and distinct acetylated forms. Our results illustrate that the application of capillary zone electrophoresis on its own or in combination with h.p.l.c. to the analysis of histones provides an important new alternative to traditional gel electrophoreses.

Capillary zone electrophoresis for haemoglobinopathy diagnosis

2012 ◽  
Vol 66 (1) ◽  
pp. 29-39 ◽  
Author(s):  
Nicole Borbely ◽  
Lorraine Phelan ◽  
Richard Szydlo ◽  
Barbara Bain
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Reference Range ◽  
Specific Reference ◽  
Specific Method ◽  
Alkaline Ph ◽  
Zone Electrophoresis ◽  
Microcolumn Chromatography ◽  
Capillary Zone

AimsCapillary zone electrophoresis (CE) at alkaline pH is increasingly used in haemoglobinopathy diagnosis. We report our evaluation of automated CE, using the Capillarys 2 Flex Piercing instrument, as a routine diagnostic method for the detection of variant haemoglobins and the diagnosis of β thalassaemia.MethodsA Capillarys 2 Flex Piercing instrument with Phoresis software was evaluated in our laboratory over a 6-week period, comparisons being made with high performance liquid chromatography (HPLC) and, for haemoglobin A2 quantification, with microcolumn chromatography.ResultsThe instrument was easy to use and was suitable for the quantification of haemoglobin A2. Quantification of A2 was precise and the percentage was stable with ageing of the blood specimen. Results differ among HPLC, CE and microcolumn chromatography and use of an instrument-specific, method-specific reference range is therefore recommended until such time as there is standardisation between methods and manufacturers. Common variant haemoglobins were provisionally identified without difficulty. There are some uncommon variant haemoglobins that are detected by HPLC but not by capillary electrophoresis, but the reverse also occurs.ConclusionsCapillary electrophoresis using a Capillarys 2 Flex Piercing instrument is suitable for haemoglobinopathy diagnosis.

scholarly journals PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN

2009 ◽  
Vol 4 (2) ◽  
pp. 48
Author(s):  
Hedi Indra Januar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Capillary Zone Electrophoresis ◽  
Immunosorbent Assay ◽  
High Performance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capillary Electrophoresis Capillary ◽  
Zone Electrophoresis ◽  
Capillary Zone

Studi ini  bertujuan  untuk  membandingkan  beberapa  metode dalam penentuan kadar amina  biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting  sebagai  standar  kualitas  produk  perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent  Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik  dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.

scholarly journals Determination of Carbohydrate-deficient Transferrin Using Capillary Zone Electrophoresis

2001 ◽  
Vol 47 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Birgitte Wuyts ◽  
Joris R Delanghe ◽  
Ishmael Kasvosve ◽  
Annick Wauters ◽  
Hugo Neels ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Sample Pretreatment ◽  
Fused Silica ◽  
Exchange Chromatography ◽  
Buffer System ◽  
Good Precision ◽  
Deming Regression ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone

Abstract Background: Current methods for carbohydrate-deficient transferrin (CDT) often suffer from low precision, complexity, or risk of false positives attributable to genetic variants. In this study, a new capillary zone electrophoresis (CZE) method for CDT was developed. Methods: CZE was performed on a P/ACE 5000 using fused-silica capillaries [50 μm (i.d.) × 47 cm] and the CEOFIX CDT buffer system with addition of 50 μL of anti-C3c and 10 μL of anti-hemoglobin. Native sera were loaded by high-pressure injection for 3 s, separated at 28 kV over 12 min, and monitored at 214 nm. Results: CDT was completely resolved by differences in migration times (di-trisialotransferrin, 9.86 ± 0.05 min; monosialotransferrin, 9.72 ± 0.05 min; asialotransferrin, 9.52 ± 0.04 min), with a CV of 0.15%. The number of theoretical plates was 312 000 ± 21 000 for the mono- and 199 000 ± 6500 for the di-trisialylated transferrin. Genetic CB and CD variants showed prominent peaks with migration times of 10.12 ± 0.06 and 9.89 ± 0.03 min, respectively, and the carbohydrate-deficient glycoprotein syndrome could be detected, excluding false-positive results. CZE results (as a percentage; y) correlated with the Axis %CDT TIATM (x) values by Deming regression analysis: y = 1.92x − 7.29; r = 0.89. CDT values in 130 healthy nonalcoholics were determined. The 2.5th and 97.5th percentiles were 1.84% and 6.79%. Conclusions: CZE without sample pretreatment can determine CDT with good precision, allows detection of variants, and correlates with ion-exchange chromatography.

A fast and validated capillary zone electrophoresis method for the determination of selected fatty acids applied to food and cosmetic purposes

2019 ◽  
Vol 11 (43) ◽  
pp. 5607-5612 ◽  
Author(s):  
Tatiane Lima Amorim ◽  
Mariana Gavioli dos Reis Pena ◽  
Fabiano Freire Costa ◽  
Marcone Augusto Leal de Oliveira ◽  
Paula Rocha Chellini
Keyword(s):  
Fatty Acids ◽  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Oleic And Linoleic Acids

The potential of capillary electrophoresis was demonstrated to the determination of fatty acids in food and cosmetic samples and a validated method is proposed to palmitic, stearic, oleic and linoleic acids quantification in these samples.

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