scholarly journals Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding

2017 ◽  
Vol 29 (6) ◽  
pp. 1460-1479 ◽  
Author(s):  
Thomas R. Vincent ◽  
Marieta Avramova ◽  
James Canham ◽  
Peter Higgins ◽  
Natasha Bilkey ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Cytosolic Calcium ◽  
Aphid Feeding

Cytosolic calcium regulates ion channels in the plasma membrane of Vicia faba guard cells

Nature ◽  
1989 ◽  
Vol 338 (6214) ◽  
pp. 427-430 ◽  
Author(s):  
Julian I. Schroeder ◽  
Susumu Hagiwara
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Vicia Faba ◽  
Guard Cells ◽  
Cytosolic Calcium

scholarly journals Single-channel Ca2+ imaging implicates Aβ1–42 amyloid pores in Alzheimer’s disease pathology

2011 ◽  
Vol 195 (3) ◽  
pp. 515-524 ◽  
Author(s):  
Angelo Demuro ◽  
Martin Smith ◽  
Ian Parker
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Ion Channels ◽  
Single Channel ◽  
Membrane Integrity ◽  
Cytosolic Calcium ◽  
Xenopus Laevis Oocytes ◽  
Permeable Membrane ◽  
Membrane Pores ◽  
Pathological Mechanism

Oligomeric forms of Aβ peptides are implicated in Alzheimer’s disease (AD) and disrupt membrane integrity, leading to cytosolic calcium (Ca2+) elevation. Proposed mechanisms by which Aβ mediates its effects include lipid destabilization, activation of native membrane channels, and aggregation of Aβ into Ca2+-permeable pores. We distinguished between these using total internal reflection fluorescence (TIRF) microscopy to image Ca2+ influx in Xenopus laevis oocytes. Aβ1–42 oligomers evoked single-channel Ca2+ fluorescence transients (SCCaFTs), which resembled those from classical ion channels but which were not attributable to endogenous oocyte channels. SCCaFTs displayed widely variable open probabilities (Po) and stepwise transitions among multiple amplitude levels reminiscent of subconductance levels of ion channels. The proportion of high Po, large amplitude SCCaFTs grew with time, suggesting that continued oligomer aggregation results in the formation of highly toxic pores. We conclude that formation of intrinsic Ca2+-permeable membrane pores is a major pathological mechanism in AD and introduce TIRF imaging for massively parallel single-channel studies of the incorporation, assembly, and properties of amyloidogenic oligomers.

Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells

2021 ◽  
Author(s):  
Md Tahjib-Ul-Arif ◽  
Shintaro Munemasa ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Yoshiyuki Murata
Keyword(s):  
Plasma Membrane ◽  
Stomatal Closure ◽  
Anion Channel ◽  
Guard Cells ◽  
Cytosolic Calcium ◽  
Peak Height ◽  
Extracellular Calcium ◽  
Wild Type ◽  
Anion Channels ◽  
In The Wild

Abstract Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild-type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild-type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

Pharmacology and Regulation of the Ion Channels of the Brown Adipocyte Plasma Membrane

1994 ◽  
pp. 103-108
Author(s):  
A. Koivisto ◽  
T. Ringer ◽  
U. Ruß ◽  
J. Nedergaard ◽  
D. Siemen
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Brown Adipocyte

scholarly journals Insulin Protects Pancreatic Acinar Cells from Cytosolic Calcium Overload and Inhibition of Plasma Membrane Calcium Pump

2011 ◽  
Vol 287 (3) ◽  
pp. 1823-1836 ◽  
Author(s):  
Parini Mankad ◽  
Andrew James ◽  
Ajith K. Siriwardena ◽  
Austin C. Elliott ◽  
Jason I. E. Bruce
Keyword(s):  
Plasma Membrane ◽  
Acinar Cells ◽  
Cytosolic Calcium ◽  
Calcium Overload ◽  
Calcium Pump ◽  
Pancreatic Acinar Cells ◽  
Plasma Membrane Calcium Pump

Ion Channels in the Plasma Membrane of Plant Cells

1992 ◽  
pp. 225-236
Author(s):  
B. R. Terry ◽  
S. D. Tyerman ◽  
G. P. Findlay
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Plant Cells

Imaging calcium entering the cytosol through a single opening of plasma membrane ion channels: SCCaFTs—fundamental calcium events

Cell Calcium ◽  
2004 ◽  
Vol 35 (6) ◽  
pp. 523-533 ◽  
Author(s):  
Hui Zou ◽  
Lawrence M Lifshitz ◽  
Richard A Tuft ◽  
Kevin E Fogarty ◽  
Joshua J Singer
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Single Opening
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