Cytosolic calcium regulates ion channels in the plasma membrane of Vicia faba guard cells

Nature ◽  
1989 ◽  
Vol 338 (6214) ◽  
pp. 427-430 ◽  
Author(s):  
Julian I. Schroeder ◽  
Susumu Hagiwara
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Vicia Faba ◽  
Guard Cells ◽  
Cytosolic Calcium

Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells

2021 ◽  
Author(s):  
Md Tahjib-Ul-Arif ◽  
Shintaro Munemasa ◽  
Toshiyuki Nakamura ◽  
Yoshimasa Nakamura ◽  
Yoshiyuki Murata
Keyword(s):  
Plasma Membrane ◽  
Stomatal Closure ◽  
Anion Channel ◽  
Guard Cells ◽  
Cytosolic Calcium ◽  
Peak Height ◽  
Extracellular Calcium ◽  
Wild Type ◽  
Anion Channels ◽  
In The Wild

Abstract Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild-type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild-type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.

scholarly journals Two Plasma Membrane H+-ATPase Genes Expressed in Guard Cells of Vicia faba Are Also Expressed Throughout the Plant

1996 ◽  
Vol 37 (5) ◽  
pp. 650-659 ◽  
Author(s):  
A. E. Hentzen ◽  
L. B. Smart ◽  
L. E. Whimmers ◽  
H. H. Fang ◽  
J. I. Schroeder ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Vicia Faba ◽  
Guard Cells

Plasma membrane ion channel regulation during abscisic acid-induced closing of stomata

1992 ◽  
Vol 338 (1283) ◽  
pp. 83-89 ◽  
Keyword(s):  
Plasma Membrane ◽  
Abscisic Acid ◽  
Ion Channels ◽  
Patch Clamp ◽  
Integrated Control ◽  
Anion Channel ◽  
Guard Cells ◽  
Anion Channels ◽  
Higher Plant ◽  
Ion Channel Regulation

The plant growth regulator abscisic acid triggers closing of stomata in the leaf epidermis in response to water stress. Recent tracer flux studies, patch-clamp studies, fluorometric Ca 2+ measurements and microelectrode experiments have provided insight into primary transduction mechanisms by which abscisic acid causes stomatal closing. Data show that abscisic acid activates non-selective Ca 2+ permeable ion channels in the plasma membrane of guard cells. The resulting elevation in the free Ca 2+ concentration in the cytosol of guard cells, and the resulting membrane depolarization as well as other unidentified Ca 2+ independent mechanisms are suggested to contribute to activation of voltage- and second messenger-dependent anion channels and outward rectifying K + channels. Recent data suggest the involvement of two types of anion channels in the regulation of stomatal movements, which provide highly distinct mechanisms for anion efflux and depolarization. A novely characterized ‘S-type’ anion channel is likely to provide a key mechanism for long-term depolarization and sustained anion efflux during closing of stomata. Patch-clamp studies have revealed the presence of a network of K + , anion and non-selective Ca 2+ -permeable channels in the plasma membrane of a higher plant cell. The integrated control of these guard cell ion channels by abscisic acid can provide control over K + and anion efflux required for stomatal closing.

scholarly journals K+ transport properties of K+ channels in the plasma membrane of Vicia faba guard cells.

1988 ◽  
Vol 92 (5) ◽  
pp. 667-683 ◽  
Author(s):  
J I Schroeder
Keyword(s):  
Plasma Membrane ◽  
Transport Properties ◽  
Vicia Faba ◽  
Guard Cell ◽  
Guard Cells ◽  
Stomatal Opening ◽  
Whole Cell ◽  
K Channels ◽  
K Transport ◽  
K Currents

Electrical properties of the plasma membrane of guard cell protoplasts isolated from stomates of Vicia faba leaves were studied by application of the whole-cell configuration of the patch-clamp technique. The two types of K+ currents that have recently been identified in guard cells may allow efflux of K+ during stomatal closing, and uptake of K+ during stomatal opening (Schroeder et al., 1987). A detailed characterization of ion transport properties of the inward-rectifying (IK+,in) and the outward-rectifying (IK+,out) K+ conductance is presented here. The permeability ratios of IK+,in and IK+,out currents for K+ over monovalent alkali metal ions were determined. The resulting permeability sequences (PK+ greater than PRb+ greater than PNa+ greater than PLi+ much greater than PCs+) corresponded closely to the ion specificity of guard cell movements in V. faba. Neither K+ currents exhibited significant inactivation when K+ channels were activated for prolonged periods (greater than 10 min). The absence of inactivation may permit long durations of K+ fluxes, which occur during guard cell movements. Activation potentials of inward K+ currents were not shifted when external K+ concentrations were changed. This differs strongly from the behavior of inward-rectifying K+ channels in animal tissue. Blue light and fusicoccin induce hyperpolarization by stimulation of an electrogenic pump. From slow-whole-cell recordings it was concluded that electrogenic pumps require cytoplasmic substrates for full activation and that the magnitude of the pump current is sufficient to drive K+ uptake through IK+,in channels. First, direct evidence was gained for the hypothesis that IK+,in channels are a molecular pathway for K+ accumulation by the finding that IK+,in was blocked by Al3+ ions, which are known to inhibit stomatal opening but not closing. The results presented in this study strongly support a prominent role for IK+,in and IK+,out channels in K+ transport across the plasma membrane of guard cells.

Ion Channels in Plants

2012 ◽  
Vol 92 (4) ◽  
pp. 1777-1811 ◽  
Author(s):  
Rainer Hedrich
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Patch Clamp ◽  
Ion Channel ◽  
Potassium Channel ◽  
Guard Cells ◽  
Channel Structure ◽  
Model Systems ◽  
Dependent Manner ◽  
Inward Rectifiers

Since the first recordings of single potassium channel activities in the plasma membrane of guard cells more than 25 years ago, patch-clamp studies discovered a variety of ion channels in all cell types and plant species under inspection. Their properties differed in a cell type- and cell membrane-dependent manner. Guard cells, for which the existence of plant potassium channels was initially documented, advanced to a versatile model system for studying plant ion channel structure, function, and physiology. Interestingly, one of the first identified potassium-channel genes encoding the Shaker-type channel KAT1 was shown to be highly expressed in guard cells. KAT1-type channels from Arabidopsis thaliana and its homologs from other species were found to encode the K+-selective inward rectifiers that had already been recorded in early patch-clamp studies with guard cells. Within the genome era, additional Arabidopsis Shaker-type channels appeared. All nine members of the Arabidopsis Shaker family are localized at the plasma membrane, where they either operate as inward rectifiers, outward rectifiers, weak voltage-dependent channels, or electrically silent, but modulatory subunits. The vacuole membrane, in contrast, harbors a set of two-pore K+ channels. Just very recently, two plant anion channel families of the SLAC/SLAH and ALMT/QUAC type were identified. SLAC1/SLAH3 and QUAC1 are expressed in guard cells and mediate Slow- and Rapid-type anion currents, respectively, that are involved in volume and turgor regulation. Anion channels in guard cells and other plant cells are key targets within often complex signaling networks. Here, the present knowledge is reviewed for the plant ion channel biology. Special emphasis is drawn to the molecular mechanisms of channel regulation, in the context of model systems and in the light of evolution.

scholarly journals Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding

2017 ◽  
Vol 29 (6) ◽  
pp. 1460-1479 ◽  
Author(s):  
Thomas R. Vincent ◽  
Marieta Avramova ◽  
James Canham ◽  
Peter Higgins ◽  
Natasha Bilkey ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Cytosolic Calcium ◽  
Aphid Feeding

Isolation of cDNA for a Plasma Membrane H+-ATPase from Guard Cells of Vicia faba L.

1995 ◽  
Vol 36 (5) ◽  
pp. 919-924 ◽  
Author(s):  
Nobuyoshi Nakajima ◽  
Hikaru Saji ◽  
Mitsuko Aono ◽  
Noriaki Kondo
Keyword(s):  
Plasma Membrane ◽  
Vicia Faba ◽  
Guard Cells ◽  
Vicia Faba L

Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of Vicia faba guard cells

2007 ◽  
Vol 86 (8) ◽  
pp. 489-500 ◽  
Author(s):  
Ulrike Homann ◽  
Tobias Meckel ◽  
Jennifer Hewing ◽  
Marc-Thorsten Hütt ◽  
Annette C. Hurst
Keyword(s):  
Plasma Membrane ◽  
Vicia Faba ◽  
Guard Cells ◽  
Fluorescent Pattern
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