scholarly journals Chloroplast Development at Low Temperatures Requires a Homolog of DIM1, a Yeast Gene Encoding the 18S rRNA Dimethylase

1998 ◽  
Vol 10 (5) ◽  
pp. 699-711 ◽  
Author(s):  
James G. Tokuhisa ◽  
Perumal Vijayan ◽  
Kenneth A. Feldmann ◽  
John A. Browse
Keyword(s):  
Low Temperatures ◽  
18S Rrna ◽  
Chloroplast Development ◽  
Yeast Gene ◽  
Gene Encoding

Chloroplast Development at Low Temperatures Requires a Homolog of DIM1, a Yeast Gene Encoding the 18S rRNA Dimethylase

1998 ◽  
Vol 10 (5) ◽  
pp. 699
Author(s):  
James G. Tokuhisa ◽  
Perumal Vijayan ◽  
Kenneth A. Feldmann ◽  
John A. Browse
Keyword(s):  
Low Temperatures ◽  
18S Rrna ◽  
Chloroplast Development ◽  
Yeast Gene ◽  
Gene Encoding

An essential yeast gene encoding a TTAGGG repeat-binding protein

1993 ◽  
Vol 13 (2) ◽  
pp. 1306-1314
Author(s):  
C Brigati ◽  
S Kurtz ◽  
D Balderes ◽  
G Vidali ◽  
D Shore
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Binding Activity ◽  
Insertion Mutation ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Cloned Gene ◽  
Diploid Cells ◽  
Ttaggg Repeat

A yeast gene encoding a DNA-binding protein that recognizes the telomeric repeat sequence TTAGGG found in multicellular eukaryotes was identified by screening a lambda gt11 expression library with a radiolabeled TTAGGG multimer. This gene, which we refer to as TBF1 (TTAGGG repeat-binding factor 1), encodes a polypeptide with a predicted molecular mass of 63 kDa. The TBF1 protein, produced in vitro by transcription and translation of the cloned gene, binds to (TTAGGG)n probes and to a yeast telomeric junction sequence that contains two copies of the sequence TTAGGG separated by 5 bp. TBF1 appears to be identical to a previously described yeast TTAGGG-repeat binding activity called TBF alpha. TBF1 produced in vitro yields protein-DNA complexes with (TTAGGG)n probes that have mobilities on native polyacrylamide gels identical to those produced by partially purified TBF alpha from yeast cells. Furthermore, when extracts are prepared from a strain containing a TBF1 gene with an antigen tag, we find that the antigen copurifies with the predominant (TTAGGG)n-binding activity in the extracts. The DNA sequence of TBF1 was determined. The predicted protein sequence suggests that TBF1 may contain a nucleotide-binding domain, but no significant similarities to any other known proteins were identified, nor was an obvious DNA-binding motif apparent. Diploid cells heterozygous for a tbf1::URA3 insertion mutation are viable but upon sporulation give rise to tetrads with only two viable spores, both of which are Ura-, indicating that the TBF1 gene is essential for growth. Possible functions of TBF1 (TFB alpha) are discussed in light of these new results.

Yeast sequencing reports.APL1, a yeast gene encoding a putative permease for basic amino acids

Yeast ◽  
1994 ◽  
Vol 10 (5) ◽  
pp. 653-657 ◽  
Author(s):  
Hana Sychrova ◽  
Marie-Renee Chevallier
Keyword(s):  
Amino Acids ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Basic Amino Acids

scholarly journals Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.

1992 ◽  
Vol 11 (13) ◽  
pp. 5111-5120 ◽  
Author(s):  
N.H. Waseem ◽  
K. Labib ◽  
P. Nurse ◽  
D.P. Lane
Keyword(s):  
Fission Yeast ◽  
Accessory Protein ◽  
Yeast Gene ◽  
Gene Encoding

Isolation, Characterization, and Disruption of the Yeast Gene Encoding Cytosolic NADP-Specific Isocitrate Dehydrogenase

Biochemistry ◽  
1994 ◽  
Vol 33 (32) ◽  
pp. 9661-9667 ◽  
Author(s):  
Thomas M. Loftus ◽  
Linda V. Hall ◽  
Sondra L. Anderson ◽  
Lee McAlister-Henn
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Yeast Gene ◽  
Gene Encoding

Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase

1994 ◽  
Vol 221 (2) ◽  
pp. 741-747 ◽  
Author(s):  
Michael HOFMANN ◽  
Eckhard BOLES ◽  
Friedrich K. ZIMMERMANN
Keyword(s):  
Yeast Gene ◽  
Gene Encoding
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