scholarly journals Terpenoid Metabolism in Wild-Type and Transgenic Arabidopsis Plants

2003 ◽  
Vol 15 (12) ◽  
pp. 2866-2884 ◽  
Author(s):  
Asaph Aharoni ◽  
Ashok P. Giri ◽  
Stephan Deuerlein ◽  
Frans Griepink ◽  
Willem-Jan de Kogel ◽  
...  
Keyword(s):  
Transgenic Arabidopsis ◽  
Wild Type ◽  
Transgenic Arabidopsis Plants ◽  
Terpenoid Metabolism

Enhanced zinc and cadmium tolerance and accumulation in transgenic Arabidopsis plants constitutively overexpressing a barley gene (HvAPX1) that encodes a peroxisomal ascorbate peroxidase

Botany ◽  
2008 ◽  
Vol 86 (6) ◽  
pp. 567-575 ◽  
Author(s):  
Weifeng Xu ◽  
Weiming Shi ◽  
Feng Liu ◽  
Akihiro Ueda ◽  
Tetsuko Takabe
Keyword(s):  
Oxidative Stress ◽  
Transgenic Plants ◽  
Ascorbate Peroxidase ◽  
Transgenic Arabidopsis ◽  
Higher Plants ◽  
Cadmium Tolerance ◽  
Wild Type ◽  
Zinc Tolerance ◽  
Zinc Stress ◽  
Transgenic Arabidopsis Plants

Ascorbate peroxidase (APX) plays an important role in oxidative stress metabolism in higher plants. To determine the role of APX in protection against excessive-zinc-induced oxidative stress, transgenic Arabidopsis plants constitutively overexpressing a peroxisomal ascorbate peroxidase gene (HvAPX1) from barley were analyzed. In this study, we found that transgenic plants were more tolerant to zinc stress than wild-type plants. Under zinc stress, the concentration of hydrogen peroxide and malondialdehyde accumulation were higher in wild-type plants than in transgenic plants. Therefore, the mechanism of zinc tolerance in transgenic plants may be due to reduced oxidative stress damage. Under zinc stress, the activities of APX were significantly higher in transgenic plants than in wild-type plants. We also found that the zinc accumulation in the shoots were much higher in transgenic plants than in wild-type plants under zinc stress. In addition, we found that compared with wild-type plants, transgenic plants were more tolerant to excessive cadmium stress and accumulated more cadmium in shoots. These results suggest that HvAPX1 plays an important role in zinc and cadmium tolerance, and might be a candidate gene for developing high-biomass tolerant plants for phytoremediation of zinc- and cadmium-polluted environments.

scholarly journals Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants

2018 ◽  
Vol 64 (No. 8) ◽  
pp. 379-385 ◽  
Author(s):  
Zhu Bo ◽  
Han Hongjuan ◽  
Fu Xiaoyan ◽  
Li Zhenjun ◽  
Gao Jianjie ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Transgenic Arabidopsis ◽  
Environmental Pollutant ◽  
Specific Rate ◽  
Wild Type Plant ◽  
Wild Type ◽  
Fresh Weight ◽  
Human Carcinogen ◽  
Specific Rate Constant ◽  
Nitroreductase Activity

The explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. TNT is toxic to many organisms, it is known to be a potential human carcinogen, and is persistent in the environment. This study presents a system of phytoremediation by Arabidopsis plants developed on the basis of overexpression of NAD(P)H-flavin nitroreductase (NFSB) from the Sulfurimonas denitrificans DSM1251. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerance and a strikingly higher capacity to remove TNT from their media. The highest specific rate constant of TNT disappearance rate was 1.219 and 2.297 mL/g fresh weight/h for wild type and transgenic plants, respectively. Meanwhile, the nitroreductase activity in transgenic plant was higher than wild type plant. All this indicates that transgenic plants show significantly enhanced tolerances to TNT; transgenic plants also exhibit strikingly higher capabilities of removing TNT from their media and high efficiencies of transformation.

Altered membrane lipase expression delays leaf senescence

2000 ◽  
Vol 28 (6) ◽  
pp. 775-777 ◽  
Author(s):  
J. Thompson ◽  
C. Taylor ◽  
T.-W. Wang
Keyword(s):  
Leaf Senescence ◽  
Cdna Clone ◽  
Transgenic Arabidopsis ◽  
Expression Library ◽  
Transgenic Arabidopsis Plants ◽  
Lipase Expression

A cDNA clone encoding a lipase that is upregulated in senescing leaves and flower petals has been isolated by screening an expression library. The abundance of the lipase mRNA increases as flowers and leaves begin to senesce, and expression of the gene is also induced by treatment with ethylene. Transgenic Arabidopsis plants in which levels of the senescence-induced lipase protein have been reduced show delayed leaf senescence.

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