scholarly journals Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in Transgenic Arabidopsis Plants

2009 ◽  
Vol 21 (2) ◽  
pp. 622-641 ◽  
Author(s):  
Hyun Kyung Lee ◽  
Seok Keun Cho ◽  
Ora Son ◽  
Zhengyi Xu ◽  
Inhwan Hwang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Transgenic Arabidopsis ◽  
E3 Ubiquitin Ligase ◽  
Membrane Anchor ◽  
Transgenic Arabidopsis Plants

Identification of Ubiquitin Ligase From Grapevine Ring C3H2C3E3 and Characterization of Drought Resistance Function of VyRCHC114

2020 ◽  
Author(s):  
Yihe Yu ◽  
Shengdi Yang ◽  
Lu Bian ◽  
Keke Yu ◽  
Xiangxuan Meng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
Phylogenetic Trees ◽  
Expression Profiles ◽  
E3 Ubiquitin Ligase ◽  
Pcr Analysis ◽  
Biotic And Abiotic Stress ◽  
Element Analysis

Abstract Background: RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type is the largest subfamily of RING, playing an important role in plants’ development and growth and their biotic and abiotic stress responses. Results: A total of 143 RING C3H2C3-type genes (RCHCs) were discovered from the grapevine genome and separated into groups (I-XI) according to their phylogenetic analysis, with these genes named according to their positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant role in the expansion of VyRCHCs family together. Structural analysis showed that most VyRCHCs(67.13%) had no more than 2 introns, while genes clustered together based on phylogenetic trees had similar motifs and evolutionarily conserved structures. Cis-acting element analysis showed the diversity of VyRCHCs regulation. The expression profiles of eight DEGs in RNA-Seq after drought stress were similar to those in qRT-PCR analysis. The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, overexpression of VyRCHC114 in Arabidopsis improved drought tolerance, moreover, the transgenic plant survival rate increased by 30%, accompanied by changing of electrolyte leakage, chlorophyll content and the activities of SOD, POD, APX and CAT were changed. AtCOR15a, AtRD29A, AtERD15 and AtP5CS1 were expressed quantitatively, the results showed that they participated in the drought stress response may be regulated by the expression of VyRCHC114.Conclusions: Valuable new information on the evolution of grapevine RCHCs and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress emerged from this research.

scholarly journals The Arabidopsis RING E3 Ubiquitin Ligase AtAIRP2 Plays Combinatory Roles with AtAIRP1 in Abscisic Acid-Mediated Drought Stress Responses

2011 ◽  
Vol 157 (4) ◽  
pp. 2240-2257 ◽  
Author(s):  
Seok Keun Cho ◽  
Moon Young Ryu ◽  
Dong Hye Seo ◽  
Bin Goo Kang ◽  
Woo Taek Kim
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
E3 Ubiquitin Ligase ◽  
Ring E3
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