scholarly journals Blue Light-Dependent in Vivo and in Vitro Phosphorylation of Arabidopsis Cryptochrome 1

2003 ◽  
Vol 15 (10) ◽  
pp. 2421-2429 ◽  
Author(s):  
D. Shalitin
Keyword(s):  
Blue Light ◽  
Cryptochrome 1

scholarly journals In vitro and in vivo Efficacy of New Blue Light Emitting Diode Phototherapy Compared to Conventional Halogen Quartz Phototherapy for Neonatal Jaundice

2005 ◽  
Vol 20 (1) ◽  
pp. 61 ◽  
Author(s):  
Yun Sil Chang ◽  
Jong Hee Hwang ◽  
Hyuk Nam Kwon ◽  
Chang Won Choi ◽  
Sun Young Ko ◽  
...  
Keyword(s):  
Blue Light ◽  
Neonatal Jaundice ◽  
Light Emitting Diode ◽  
In Vivo Efficacy ◽  
Light Emitting

scholarly journals Trp triad-dependent rapid photoreduction is not required for the function of Arabidopsis CRY1

2015 ◽  
Vol 112 (29) ◽  
pp. 9135-9140 ◽  
Author(s):  
Jie Gao ◽  
Xu Wang ◽  
Meng Zhang ◽  
Mingdi Bian ◽  
Weixian Deng ◽  
...  
Keyword(s):  
Wild Type ◽  
Evolutionarily Conserved ◽  
Tryptophan Residues ◽  
Electron Transportation ◽  
Cryptochrome 1 ◽  
Evolutionary Lineages ◽  
Transcription Repressors

Cryptochromes in different evolutionary lineages act as either photoreceptors or light-independent transcription repressors. The flavin cofactor of both types of cryptochromes can be photoreduced in vitro by electron transportation via three evolutionarily conserved tryptophan residues known as the “Trp triad.” It was hypothesized that Trp triad-dependent photoreduction leads directly to photoexcitation of cryptochrome photoreceptors. We tested this hypothesis by analyzing mutations of Arabidopsis cryptochrome 1 (CRY1) altered in each of the three Trp-triad tryptophan residues (W324, W377, and W400). Surprisingly, in contrast to a previous report all photoreduction-deficient Trp-triad mutations of CRY1 remained physiologically and biochemically active in Arabidopsis plants. ATP did not enhance rapid photoreduction of the wild-type CRY1, nor did it rescue the defective photoreduction of the CRY1W324A and CRY1W400F mutants that are photophysiologically active in vivo. The lack of correlation between rapid flavin photoreduction or the effect of ATP on the rapid flavin photoreduction and the in vivo photophysiological activities of plant cryptochromes argues that the Trp triad-dependent photoreduction is not required for the function of cryptochromes and that further efforts are needed to elucidate the photoexcitation mechanism of cryptochrome photoreceptors.

scholarly journals Antimicrobial Blue Light Inactivation of Gram-Negative Pathogens in Biofilms: In Vitro and In Vivo Studies

2016 ◽  
Vol 213 (9) ◽  
pp. 1380-1387 ◽  
Author(s):  
Yucheng Wang ◽  
Ximing Wu ◽  
Jia Chen ◽  
Rehab Amin ◽  
Min Lu ◽  
...  
Keyword(s):  
Blue Light ◽  
In Vivo Studies ◽  
Gram Negative

scholarly journals Blue Light Rescues Mice from Potentially Fatal Pseudomonas aeruginosa Burn Infection: Efficacy, Safety, and Mechanism of Action

2012 ◽  
Vol 57 (3) ◽  
pp. 1238-1245 ◽  
Author(s):  
Tianhong Dai ◽  
Asheesh Gupta ◽  
Ying-Ying Huang ◽  
Rui Yin ◽  
Clinton K. Murray ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Infected Mouse ◽  
Blue Light ◽  
Mouse Skin ◽  
Light Therapy ◽  
Time Curve ◽  
Safe Alternative ◽  
Content Type

ABSTRACTBlue light has attracted increasing attention due to its intrinsic antimicrobial effect without the addition of exogenous photosensitizers. However, the use of blue light for wound infections has not been established yet. In this study, we demonstrated the efficacy of blue light at 415 nm for the treatment of acute, potentially lethalPseudomonas aeruginosaburn infections in mice. Ourin vitrostudies demonstrated that the inactivation rate ofP. aeruginosacells by blue light was approximately 35-fold higher than that of keratinocytes (P= 0.0014). Transmission electron microscopy revealed blue light-mediated intracellular damage toP. aeruginosacells. Fluorescence spectroscopy suggested that coproporphyrin III and/or uroporphyrin III are possibly the intracellular photosensitive chromophores associated with the blue light inactivation ofP. aeruginosa.In vivostudies using anin vivobioluminescence imaging technique and an area-under-the-bioluminescence-time-curve (AUBC) analysis showed that a single exposure of blue light at 55.8 J/cm2, applied 30 min after bacterial inoculation to the infected mouse burns, reduced the AUBC by approximately 100-fold in comparison with untreated and infected mouse burns (P< 0.0001). Histological analyses and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assays indicated no significant damage in the mouse skin exposed to blue light at the effective antimicrobial dose. Survival analyses revealed that blue light increased the survival rate of the infected mice from 18.2% to 100% (P< 0.0001). In conclusion, blue light therapy might offer an effective and safe alternative to conventional antimicrobial therapy forP. aeruginosaburn infections.

Bactericidal effects research of 470 nm blue light on Pseudomonas aeruginosa: in vitro and in vivo research

Laser Physics ◽  
2019 ◽  
Vol 29 (3) ◽  
pp. 035601
Author(s):  
Benjian Shen ◽  
Liucun Gao ◽  
Jin Xing ◽  
Jing Fang ◽  
Jie Liang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Blue Light ◽  
Bactericidal Effects
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