scholarly journals Barley Acyl Carrier Protein II: Nucleotide Sequence of cDNA Clones and Chromosomal Location of the Acl2 Gene

1991 ◽  
Vol 97 (1) ◽  
pp. 472-474 ◽  
Author(s):  
Lars Hansen ◽  
Sakari Kauppinen
Keyword(s):  
Nucleotide Sequence ◽  
Chromosomal Location ◽  
Acyl Carrier Protein ◽  
Cdna Clones ◽  
Carrier Protein

scholarly journals Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti

1992 ◽  
Vol 283 (2) ◽  
pp. 321-326 ◽  
Author(s):  
A R Slabas ◽  
D Chase ◽  
I Nishida ◽  
N Murata ◽  
C Sidebottom ◽  
...  
Keyword(s):  
Gene Product ◽  
Rhizobium Meliloti ◽  
Acyl Carrier Protein ◽  
Amino Acid Sequences ◽  
Cdna Clones ◽  
Small Range ◽  
Carrier Protein ◽  
Coding Region ◽  
Higher Plant ◽  
Signal Molecule

cDNA clones encoding the fatty-acid- biosynthetic enzyme NADPH-linked 3-oxoacyl-(acyl carrier protein) (ACP) reductase were isolated from a Brassica napus (rape) developing seed library and from an Arabidopsis thaliana (thale cress) leaf library. The N-terminal end of the coding region shows features typical of a stromal-targeting plastid-transit peptide. The deduced amino acid sequences have 41% and 55% identity respectively with the nodG-gene product of Rhizobium meliloti, one of the host-specific genes that restrict infectivity of this bacterium to a small range of host plants. The probability that the nodG-gene product is a oxoreductase strengthens the hypothesis that some of the host-specific nod-gene products are enzymes which synthesize polyketides that uniquely modify the Rhizobium nodulation signal molecule.

scholarly journals Isolation and Characterization of Two Safflower Oleoyl-Acyl Carrier Protein Thioesterase cDNA Clones

1992 ◽  
Vol 100 (4) ◽  
pp. 1751-1758 ◽  
Author(s):  
Deborah S. Knutzon ◽  
Janice L. Bleibaum ◽  
Janet Nelsen ◽  
Jean C. Kridl ◽  
Gregory A. Thompson
Keyword(s):  
Acyl Carrier Protein ◽  
Cdna Clones ◽  
Carrier Protein ◽  
Isolation And Characterization

scholarly journals Isolation of cDNAs from Brassica napus encoding the biotin-binding and transcarboxylase domains of acetyl-CoA carboxylase: assignment of the domain structure in a full-length Arabidopsis thaliana genomic clone

1994 ◽  
Vol 301 (2) ◽  
pp. 599-605 ◽  
Author(s):  
K M Elborough ◽  
R Swinhoe ◽  
R Winz ◽  
J T Kroon ◽  
L Farnsworth ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Acyl Carrier Protein ◽  
Fatty Acid Synthetase ◽  
Genomic Clone ◽  
Full Length ◽  
Cdna Clones ◽  
Carrier Protein ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Biotin Binding

One independent and two overlapping rape cDNA clones have been isolated from a rape embryo library. We have shown that they encode a 2.3 kb and a 2.5 kb stretch of the full-length acetyl-CoA carboxylase (ACCase) cDNA, corresponding to the biotin-binding and transcarboxylase domains respectively. Using the cDNA in Northern-blot analysis we have shown that the mRNA for ACCase has a higher level of expression in rape seed than in rape leaf and has a full length of 7.5 kb. The level of expression during rape embryogenesis was compared with both oil deposition and expression of two fatty acid synthetase components enoyl-(acyl-carrier-protein) reductase and 3-oxoacyl-(acyl-carrier-protein) reductase. Levels of ACCase mRNA were shown to peak at 29 days after anthesis during embryonic development, similarly to enoyl-(acyl-carrier-protein) reductase and 3-oxoacyl-(acyl-carrier-protein) reductase mRNA. In addition, a full-length genomic clone (19 kb) of Arabidopsis ACCase has been isolated and partially sequenced. Analysis of the clone has allowed the first plant ACCase activity domains (biotin carboxylase-biotin binding-transcarboxylase) to be ordered and assigned. Southern-blot analysis using the Arabidopsis clone indicates that ACCase is a single-copy gene in Arabidopsis but is encoded by a small gene family in rape.

scholarly journals Nucleotide Sequence of a Stearoyl-Acyl Carrier Protein Desaturase cDNA from Developing Seeds of Rice

1995 ◽  
Vol 108 (2) ◽  
pp. 845-846 ◽  
Author(s):  
H. Akagi ◽  
T. Baba ◽  
H. Shimada ◽  
T. Fujimura
Keyword(s):  
Nucleotide Sequence ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Developing Seeds

scholarly journals cDNA Clones Encoding [beta]-Ketoacyl-Acyl Carrier Protein Synthase III from Cuphea wrightii

1995 ◽  
Vol 108 (1) ◽  
pp. 443-444 ◽  
Author(s):  
M. B. Slabaugh ◽  
H. Tai ◽  
J. Jaworski ◽  
S. J. Knapp
Keyword(s):  
Acyl Carrier Protein ◽  
Cdna Clones ◽  
Carrier Protein
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