scholarly journals Nucleotide Sequence of a Complementary DNA Clone Encoding Stearoyl-Acyl Carrier Protein Desaturase from Castor Bean, Ricinus communis

1991 ◽  
Vol 96 (1) ◽  
pp. 344-345 ◽  
Author(s):  
Deborah S. Knutzon ◽  
Donna E. Scherer ◽  
William E. Schreckengost
Keyword(s):  
Nucleotide Sequence ◽  
Castor Bean ◽  
Acyl Carrier Protein ◽  
Ricinus Communis ◽  
Carrier Protein ◽  
Complementary Dna

scholarly journals Plant holo-(acyl carrier protein) synthase

1988 ◽  
Vol 252 (1) ◽  
pp. 39-45 ◽  
Author(s):  
S A Elhussein ◽  
J A Miernyk ◽  
J B Ohlrogge
Keyword(s):  
Spinacia Oleracea ◽  
Acyl Carrier Protein ◽  
Ricinus Communis ◽  
Plant Cells ◽  
Gel Permeation Chromatography ◽  
Carrier Protein ◽  
Improved Method ◽  
Ph Optimum ◽  
Spinach Leaf

1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The partially purified enzyme had a pH optimum of 8.2 and Km values of 2 microM, 72 microM and 3 mM for apo-(acyl carrier protein), CoA and Mg2+ respectively. Synthase activity was inhibited in vitro by the reaction product 3′,5′-ADP. 4. Results from the fractionation of spinach leaf and developing castor-oil-seed (Ricinus communis) endosperm cells were consistent with a cytosolic localization of holo-(acyl carrier protein) synthase activity in plant cells.

scholarly journals Nucleotide Sequence of a Stearoyl-Acyl Carrier Protein Desaturase cDNA from Developing Seeds of Rice

1995 ◽  
Vol 108 (2) ◽  
pp. 845-846 ◽  
Author(s):  
H. Akagi ◽  
T. Baba ◽  
H. Shimada ◽  
T. Fujimura
Keyword(s):  
Nucleotide Sequence ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Developing Seeds

Cloning and Bioinformatic Analysis of Stearoyl-Acyl-Carrier Protein Desaturase Gene in Cassava (Manihot esculenta Crantz)

2014 ◽  
Vol 1010-1012 ◽  
pp. 155-159
Author(s):  
Jie Fan ◽  
Rui Mei Li ◽  
Meng Ting Geng ◽  
Yuan Yao ◽  
Cheng Long Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Manihot Esculenta ◽  
Unsaturated Fatty Acids ◽  
Acyl Carrier Protein ◽  
Ricinus Communis ◽  
3D Structure ◽  
Bioinformatic Analysis ◽  
Ion Binding ◽  
Carrier Protein ◽  
Desaturase Gene

Stearoyl-acyl-carrier protein desaturase is one of the key enzymes that irreversibly catalyze the biosynthesis of fatty acids, and has an influence on the proportion of saturated and unsaturated fatty acids. In this research,MeSACPDhas been cloned from cassava, with 1197 nucleotides and 398 amino acids, respectively. It has high homologous with the stearoyl-acyl-carrier protein desaturase inRicinus communis. The 3D structure ofMeSACPDshow a conserve motif related to Fe ion binding. We suppose the motif may be the active center and the substrate binding site area of stearoyl-acyl-carrier protein desaturase.

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