scholarly journals Products Released from Enzymically Active Cell Wall Stimulate Ethylene Production and Ripening in Preclimacteric Tomato (Lycopersicon esculentum Mill.) Fruit

1988 ◽  
Vol 88 (4) ◽  
pp. 1037-1041 ◽  
Author(s):  
Jeffrey K. Brecht ◽  
Donald J. Huber
Keyword(s):  
Cell Wall ◽  
Lycopersicon Esculentum ◽  
Ethylene Production ◽  
Active Cell ◽  
Lycopersicon Esculentum Mill

scholarly journals Analysis of the molecular size of tomato (Lycopersicon esculentum Mill) fruit polyuronides by gel filtration and low-speed sedimentation equilibrium

1987 ◽  
Vol 245 (2) ◽  
pp. 463-466 ◽  
Author(s):  
G B Seymour ◽  
S E Harding
Keyword(s):  
Cell Wall ◽  
Lycopersicon Esculentum ◽  
Tomato Fruit ◽  
Gel Filtration ◽  
Molecular Size ◽  
Sedimentation Equilibrium ◽  
Low Speed ◽  
Lycopersicon Esculentum Mill ◽  
Wall Structures ◽  
Molecular Size Distribution

The cell-wall structures of tomato (Lycopersicon esculentum Mill) and other fruit are intimately linked with the nature of their polyuronides. Cell-wall polyuronides from unripe and ripe tomato fruit were isolated and purified and their molecular size and molecular-size distributions were compared. It was demonstrated that there is a considerable decrease in the weight-average Mr upon ripening (from 160,000 +/- 10,000 to 96,000 +/- 4000) and a corresponding increase in polydispersity, particularly at the low-Mr end of the distribution. The estimates of polyuronide molecular size and molecular-size distribution were obtained without the need for polyuronide standards of known Mr by using gel-filtration chromatography combined with the absolute method of low-speed sedimentation equilibrium.

scholarly journals Mobility Limitations of Bound Polygalacturonase in Isolated Cell Wall from Tomato Pericarp Tissue

1990 ◽  
Vol 115 (1) ◽  
pp. 97-101 ◽  
Author(s):  
James W. Rushing ◽  
Donald J. Huber
Keyword(s):  
Cell Wall ◽  
Lycopersicon Esculentum ◽  
Cell Walls ◽  
Active Cell ◽  
Similar Data ◽  
Mobility Limitations ◽  
No Release ◽  
Autolytic Activity ◽  
Isolated Cell

Enzymically active cell wall isolated from mature-green and ripening tomato (Lycopersicon esculentum Mill cv. `Rutgers') fruit was employed to investigate the mobility of the enzyme polygalacturonase (PG, EC 3.2.1.15). Cell walls from mature-green `Rutgers' fruit or from the ripening mutant rin, which alone exhibits little or no release of pectin, were unaffected by the addition of enzymically active cell wall from ripening `Rutgers' fruit, indicating that PG is either not transferred at all or is not transferred to sites of pectin hydrolysis. The quantity of pectin released by the addition of soluble PG to enzymically active wall depended on the quantity of enzyme added. Similar data were obtained using purified PG2. Pectin solubilization from all wall isolates exhibiting enzymically mediated pectin release diminished with time; however, transfer to fresh buffer initiated a resumption of autolytic activity, indicating that an inhibitor is released during the course of pectin hydrolysis.

scholarly journals CELL WALL SYNTHESIS IN `RUTGERS', RIN AND NOR TOMATO FRUIT

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1083e-1083
Author(s):  
Elizabeth J. Mitcham ◽  
Kenneth C. Gross ◽  
Timothy J Ng
Keyword(s):  
Cell Wall ◽  
Lycopersicon Esculentum ◽  
Cell Walls ◽  
Tomato Fruit ◽  
Experimental Period ◽  
Fruit Color ◽  
Transient Increase ◽  
Cell Wall Synthesis ◽  
B Values ◽  
Lycopersicon Esculentum Mill

Cell wall synthesis during development and ripening of `Rutgers', rin and nor tomato (Lycopersicon esculentum Mill.) fruit was quantified by monitoring incorporation of 14C into outer pericarp cell walls after pedicel injection of (U-14C) - sucrose. Fruit color (Hunter “a” and “b” values) and firmness (Instron) were also monitored. 14C-Incorporation continued throughout development and ripening in `Rutgers' cell walls and exhibited a transient increase from late maturegreen to the turning stage. Incorporation of 14C into cell walls of rin pericarp tissue was similar to `Rutgers' at 20 days pest-anthesls (DPA) (immature-green) but decreased to a level similar to red `Rutgers' fruit by 35 DPA. Incorporation of 14C into nor pericarp cell walls was low throughout the experimental period (20 to 75 DPA). In contrast to previous reports, rin and nor pericarp tissue exhibitad a decrease in firmness of the outer pericarp. However, the rate of softening was slower than in `Rutgers'. Pericarp tissue from rin and nor fruit at 70 and 75 DPA, respectively, resisted compression as much as pink `Rutgers' pericarp tissue.

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