scholarly journals Purification and Characterization of Two Benzoyl-l-Tyrosine p-Nitroanilide Hydrolases from Etiolated Leaves of Zea mays L

1987 ◽  
Vol 84 (3) ◽  
pp. 770-774 ◽  
Author(s):  
Michio Doi ◽  
Yuzo Shioi
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Purification And Characterization ◽  
Etiolated Leaves

scholarly journals Purification and Characterization of Porin from Corn (Zea mays L.) Mitochondria

1993 ◽  
Vol 102 (2) ◽  
pp. 615-621 ◽  
Author(s):  
J. A. Aljamal ◽  
G. Genchi ◽  
V. De Pinto ◽  
L. Stefanizzi ◽  
A. De Santis ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Purification And Characterization

Purification and Characterization of a β-Glucosidase Specific for 2,4-Dihydroxy- 7-methoxy-1,4-benzoxazin-3-one (DIMBOA) Glucoside in Maize

1999 ◽  
Vol 54 (3-4) ◽  
pp. 181-185 ◽  
Author(s):  
Akira Oikawa ◽  
Kenkichi Ebisui ◽  
Masayuki Sue ◽  
Atsushi Ishihara ◽  
Hajime Iwamura
Keyword(s):  
Zea Mays ◽  
Ion Exchange ◽  
Cation Exchange ◽  
Hydroxamic Acid ◽  
Zea Mays L ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Purification And Characterization ◽  
Apparent Homogeneity

Occurrence and properties of hydroxamic acid glucoside glucosidase were investigated in 10-day-old, autotrophic maize (Zea mays L.) in which 2,4-dihydroxy-7-methoxy-1,4- benzoxazin-3-one glucoside (DIMBOA-G) is a major benzoxazinone component. Crude extracts of both leaves and roots showed glucosidase activity for both DIMBOA-G and 2,4- dihydroxy-1,4-benzoxazin-3-one glucoside (DIBOA-G). A cation-exchange chromatography after cryoprecipitation of the extract from leaves gave a peak with both activities, and further purification by ion-exchange and hydroxyapatite chromatography gave a fraction with an apparent homogeneity, the purification being 560 fold. The Km values (mᴍ) of the purified glucosidase were 0.16 for DIMBOA-G, 0.68 for DIBOA-G and 2.96 for p-nitrophenyl-β-ᴅ-glucopyranoside. The activity on salicin and esculin was too low to be detected. The data indicate that a glucosidase specific for DIMBOA-G comes into contact with constitutive benzoxazinone glucosides producing defensive aglycone when plants are damaged by microbial or insect attacks.

scholarly journals Characterization of phenylpropanoid pathway genes within European maize (Zea mays L.) inbreds

2008 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Jeppe Andersen ◽  
Imad Zein ◽  
Gerhard Wenzel ◽  
Birte Darnhofer ◽  
Joachim Eder ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Phenylpropanoid Pathway ◽  
European Maize
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