scholarly journals The Simultaneous Determination of Carbon Dioxide Release and Oxygen Uptake in Suspensions of Plant Leaf Mitochondria Oxidizing Glycine

1987 ◽  
Vol 83 (2) ◽  
pp. 349-353 ◽  
Author(s):  
Ross McC. Lilley ◽  
Holger Ebbighausen ◽  
Hans W. Heldt
1987 ◽  
Vol 33 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Mikio Chiba ◽  
Alan W. Bown ◽  
David Danic

The inhibition of yeast (Saccharomyces cerevesiae) metabolism by fungicidal chemicals was investigated. Glucose- or ethanol-dependent yeast respiration was measured with an oxygen electrode, and manometric determination of carbon dioxide release was used to measure fermentation. Both respiration and fermentation were inhibited more by benomyl than by identical molar concentrations of its breakdown product, carbendazim. Butyl isocyanate, another benomyl breakdown product, inhibited respiration more but inhibited fermentation less than the parent compound. Of the isocyanates tested, hexyl isocyanate was the most inhibitory towards both activities. Captan was more active and iprodione less active than benomyl. Because benomyl rapidly broke down to carbendazim when it was prepared in 80% ethanol, only 59% of the dissolved benomyl was intact when it was added to yeast to determine its effect on respiration or fermentation.


1992 ◽  
Vol 38 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Keisuke TSUJI ◽  
Masatoshi SHIMIZU ◽  
Yoshinori NISHIMURA ◽  
Yasue NAKAGAWA ◽  
Tomio ICHIKAWA

2019 ◽  
Vol 967 ◽  
pp. 171-178 ◽  
Author(s):  
Andriana Surleva ◽  
Veronika Ivanova

An ion chromatographic method for determination of bromide and bromate in the presence of standard anions in drinking water was described. The method was based on separation on Metrosep A Supp 7-250 (250 x 4 mm) column with 3.6 mmol/L Na2CO3 as eluent and conductivity detection after sequential chemical and carbon dioxide suppression. The influence of flow rate and column temperature was studied and optimal experimental conditions for simultaneous determination of eight anions were chosen. Analytical characteristics of the ion chromatographic method were assessed for simultaneous determination of bromide, bromate, fluoride, chloride, nitrate, nitrite, phosphate and sulfate. The calibration curves were linear (r2=1, N=7) in the concentration ranges: 0.4-12 mg/L BrO3- and 0.3-11 mg/L Br-. Recovery test was performed on a spiked certified reference material for soft drinking water. The obtained recoveries for bromate and bromide were 96.0 and 101.0 %, respectively. The repeatability and intermediate precision were between 5.1 and 0.14 % (RSD) depending on the analytes concentration. The limits of detection were 20 μg/L BrO3- and 10 μg/L Br-


1979 ◽  
Vol 184 (2) ◽  
pp. 457-460 ◽  
Author(s):  
G P Arron ◽  
M H Spalding ◽  
G E Edwards

Mitochondria isolated from spinach (Spinacia oleracea) leaves oxidized glycine with a stoichiometry of CO2 evolution to O2 uptake of 2 : 1. In the absence of added substrate, the mitochondria exhibited an extremely low endogenous rate of O2 uptake.


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