Determination of in vivo oxygen uptake and carbon dioxide evolution rates from off-gas measurements under highly dynamic conditions

2002 ◽  
Vol 81 (4) ◽  
pp. 448-458 ◽  
Author(s):  
L. Wu ◽  
H. C. Lange ◽  
W. M. van Gulik ◽  
J. J. Heijnen
1959 ◽  
Vol 37 (5) ◽  
pp. 803-813 ◽  
Author(s):  
R. W. Shuel ◽  
S. E. Dixon

Measurements were made of oxygen uptake and carbon dioxide evolution, during the first 24 hours of life, by larvae on substrates of different age and type. These substrates included royal jelly, the pharyngeal secretion fed to larvae destined to become queens, which varied in age from 0 to 96 hours; the pharyngeal secretion fed to young larvae destined to be workers, which varied in age from 0 to 60 hours and which was termed "worker jelly"; and the food supplied to older worker larvae, composed of a pharyngeal secretion modified by an admixture of honey and pollen, and termed "modified jelly". The pattern and magnitude of oxygen uptake was similar on all substrates. Net carbon dioxide evolution by larvae on royal jelly or modified jelly was highly positive; net carbon dioxide evolution by larvae on worker jelly was slightly negative. Microchemical analyses showed that royal jelly differed in composition from worker jelly and modified jelly. The composition of royal jelly remained relatively constant with age. The addition of sugars to worker jelly produced an increase in carbon dioxide output which was nullified by the further addition of an extract of the water-soluble acids of royal jelly. The differences observed in carbon dioxide evolution by young larvae on worker jelly and royal jelly are considered to be an expression of the initiation of female dimorphism. An hypothesis of nutritional balance is advanced to account for this dimorphism.


1956 ◽  
Vol 34 (1) ◽  
pp. 54-59 ◽  
Author(s):  
E. R. Waygood ◽  
Ann Oaks ◽  
G. A. Maclachlan

Partially purified enzyme preparations of wheat leaves oxidize indoleacetic acid completely in the presence of manganese and a monohydroxyphenol or resorcinol. A characteristic feature of the oxygen uptake is an induction period followed by a rapid oxygen uptake which ceases when 1 mole of oxygen is consumed per mole indoleacetic acid added. One mole of carbon dioxide is evolved per mole indoleacetic acid. There is no lag in carbon dioxide evolution, but it is not given off anaerobically. Oxygen, blue light, and acetaldehyde overcome the induction period. Hydrogen peroxide formed in a final aerobic dehydrogenase step is utilized to provide a source of manganic ions which act as an electron acceptor in an initial oxidative step.


1966 ◽  
Vol 38 (7) ◽  
pp. 500-509 ◽  
Author(s):  
C. PRYS-ROBERTS ◽  
G.R. KELMAN ◽  
J.F. NUNN
Keyword(s):  

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