scholarly journals Induction of Heat Shock Protein Messenger RNA in Maize Mesocotyls by Water Stress, Abscisic Acid, and Wounding

1984 ◽  
Vol 76 (1) ◽  
pp. 270-274 ◽  
Author(s):  
J. J. Heikkila ◽  
J. E. T. Papp ◽  
G. A. Schultz ◽  
J. Derek Bewley
Keyword(s):  
Abscisic Acid ◽  
Water Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Messenger Rna

Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers

1996 ◽  
Vol 37 (2) ◽  
pp. 443-449 ◽  
Author(s):  
J. E. Gabriel ◽  
J. A. Ferro ◽  
R. M. P. Stefani ◽  
M. I. T. Ferro ◽  
S. L. Gomes ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Heat Shock Protein 70 ◽  
Messenger Rna ◽  
Acute Heat Stress ◽  
Heat Shock Protein Expression

A comparison of the stress responses of Zea mays seedlings as shown by qualitative changes in protein synthesis

1988 ◽  
Vol 66 (9) ◽  
pp. 1883-1890 ◽  
Author(s):  
P. C. Bonham-Smith ◽  
M. Kapoor ◽  
J. D. Bewley
Keyword(s):  
Protein Synthesis ◽  
Abscisic Acid ◽  
Water Stress ◽  
Heat Shock ◽  
Stress Responses ◽  
Protein Profile ◽  
Tissue Response ◽  
Specific Proteins ◽  
Induced Proteins ◽  
Qualitative Changes

Maize seedlings respond to heat shock, water stress, abscisic acid treatment, and wounding with the synthesis of stress-specific proteins. Unlike the almost instantaneous (10 min) heat-shock response, a much longer stress exposure is required before the synthesis of water stress induced, abscisic acid induced, or wound-induced proteins. As with heat shock, the protein profile of 24 h water stress induced proteins is consistent between tissue types, whereas seedling tissue response to wounding or heavy metals varies. Wounding of the mesocotyl for 12 h or more results in a complex change (induction and inhibition) in protein synthesis in the growing region, while protein synthesis in the nongrowing region is affected to a much lesser extent.

Preoperative evaluation of heat shock protein 47 expression to identify patients with colorectal cancer with lymph node metastasis and poor prognosis.

2017 ◽  
Vol 35 (4_suppl) ◽  
pp. 546-546
Author(s):  
Koichiro Mori ◽  
Yuji Toiyama ◽  
Yoshinaga Okugawa ◽  
Takashi Ichikawa ◽  
Yuka Nagano ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Node ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Mrna Expression ◽  
Messenger Rna ◽  
Poor Prognosis ◽  
University Hospital ◽  
Heat Shock Protein 47 ◽  
Node Metastasis

546 Background: Accumulating evidences reveal that overexpression of Heat shock protein 47 (HSP47) increase cancer progression, and that HSP47 expression in the tumor-associated stroma serve as diagnostic marker in various cancers. In addition, we recently performed immunohistochemistry to evaluate HSP47 expression in surgical colorectal cancer (CRC) specimens, and showed that the count of HSP47 positive spindle cell in cancer stroma was significantly associated with lymph node (LN) metastasis, early recurrence and poor prognosis in CRC patients. Thus, evaluating HSP47 expression in CRCs preoperatively may be valuable for planning the treatment of patients with CRC. In this study, we quantified the messenger RNA(mRNA) expression of HSP47 in CRCs by using preoperative biopsy samples, and analyzed the association between HSP47 mRNA expression and clinico-pathological factors including prognosis in patients with CRC. Methods: A total of 139 CRC samples, which were taken by biopsy before surgery at Mie University Hospital from 2000 to 2005, were enrolled. HSP47 gene expression was determined by quantitative real-time reverse transcription–PCR using Power SYBR Green PCR methods. Results: All CRC patients were classified according to the tumor-node-metastasis (TNM) classification system as follows: stage I (n = 33); stage II (n = 44); stage III (n = 33), and stage IV (n = 29). High HSP47 expression in CRC was significantly associated with higher T stage (p = 0.0163), LN metastasis (p = 0.0186), vessel invasion (p = 0.0328) and higher TNM staging (p = 0.0115). Kaplan-Meier analysis showed that patients with high HSP47 expression had significantly poorer overall survival (OS) than those with low (p = 0.0003). Furthermore, multivariate analyses revealed that HSP47 expression was an independent predictive marker for LN metastasis and poor OS in CRC patients, respectively (LN metastasis; OR; 2.3946, p = 0.0249, OS; HR; 2.7407, p = 0.00224). Conclusions: In conclusion, quantification of HSP47 expression using biopsy samples can identify the CRC patients who may suffer from LN metastasis and poor prognosis, preoperatively.

Sign in / Sign up

Export Citation Format

Share Document