The PstI polymorphism of the endotoxin-inducible heat-shock protein 70-2 gene does not affect messenger RNA level in human whole-blood cultures

2000 ◽  
Vol 26 (8) ◽  
pp. 1139-1143 ◽  
Author(s):  
S. Schroeder ◽  
M. Reck ◽  
L. Eric Lehmann ◽  
M. Book ◽  
A. Hoeft ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Whole Blood ◽  
Heat Shock Protein 70 ◽  
Messenger Rna ◽  
Blood Cultures ◽  
Human Whole Blood

Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers

1996 ◽  
Vol 37 (2) ◽  
pp. 443-449 ◽  
Author(s):  
J. E. Gabriel ◽  
J. A. Ferro ◽  
R. M. P. Stefani ◽  
M. I. T. Ferro ◽  
S. L. Gomes ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Heat Shock Protein 70 ◽  
Messenger Rna ◽  
Acute Heat Stress ◽  
Heat Shock Protein Expression

scholarly journals Heat shock protein 70 regulates platelet integrin activation, granule secretion and aggregation

2016 ◽  
Vol 310 (7) ◽  
pp. C568-C575 ◽  
Author(s):  
Rachel A. Rigg ◽  
Laura D. Healy ◽  
Marie S. Nowak ◽  
Jérémy Mallet ◽  
Marisa L. D. Thierheimer ◽  
...  
Keyword(s):  
T Cells ◽  
Platelet Aggregation ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Platelet Activation ◽  
Whole Blood ◽  
Heat Shock Protein 70 ◽  
Ex Vivo ◽  
Granule Secretion ◽  
And Function

Molecular chaperones that support protein quality control, including heat shock protein 70 (Hsp70), participate in diverse aspects of cellular and physiological function. Recent studies have reported roles for specific chaperone activities in blood platelets in maintaining hemostasis; however, the functions of Hsp70 in platelet physiology remain uninvestigated. Here we characterize roles for Hsp70 activity in platelet activation and function. In vitro biochemical, microscopy, flow cytometry, and aggregometry assays of platelet function, as well as ex vivo analyses of platelet aggregate formation in whole blood under shear, were carried out under Hsp70-inhibited conditions. Inhibition of platelet Hsp70 blocked platelet aggregation and granule secretion in response to collagen-related peptide (CRP), which engages the immunoreceptor tyrosine-based activation motif-bearing collagen receptor glycoprotein VI (GPVI)-Fc receptor-γ chain complex. Hsp70 inhibition also reduced platelet integrin-αIIbβ3activation downstream of GPVI, as Hsp70-inhibited platelets showed reduced PAC-1 and fibrinogen binding. Ex vivo, pharmacological inhibition of Hsp70 in human whole blood prevented the formation of platelet aggregates on collagen under shear. Biochemical studies supported a role for Hsp70 in maintaining the assembly of the linker for activation of T cells signalosome, which couples GPVI-initiated signaling to integrin activation, secretion, and platelet function. Together, our results suggest that Hsp70 regulates platelet activation and function by supporting linker for activation of T cells-associated signaling events downstream of platelet GPVI engagement, suggesting a role for Hsp70 in the intracellular organization of signaling systems that mediate platelet secretion, “inside-out” activation of platelet integrin-αIIbβ3, platelet-platelet aggregation, and, ultimately, hemostatic plug and thrombus formation.

Heat shock protein 70 messenger RNA reflects the severity of ischemia/hypoxia-reperfusion injury in the perfused rat liver

1997 ◽  
Vol 25 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Tomohiko Aoe ◽  
Hideo Inaba ◽  
Shinpei Kon ◽  
Mie Imai ◽  
Mituo Aono ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Reperfusion Injury ◽  
Rat Liver ◽  
Heat Shock Protein 70 ◽  
Messenger Rna ◽  
Perfused Rat Liver

scholarly journals Heat Shock Protein 70 Messenger RNA in Rat Leukocytes Elevates After Severe Intestinal Ischemia–Reperfusion

2019 ◽  
Vol 242 ◽  
pp. 342-348 ◽  
Author(s):  
Yuka Mine ◽  
Fumihiko Fujita ◽  
Takehiko Murase ◽  
Shinichiro Ito ◽  
Mitsuhisa Takatsuki ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Messenger Rna ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Intestinal Ischemia Reperfusion

Ammonia aggravates emotional stress-induced gastric mucosal oxidative injury through the cancellation of heat shock protein 70 (HSP70)

2001 ◽  
Vol 120 (5) ◽  
pp. A152-A152
Author(s):  
H SUZUKI ◽  
S NAGAHASHI ◽  
M MIYAZAWA ◽  
M MORI ◽  
H NAGATA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Emotional Stress ◽  
Heat Shock Protein 70 ◽  
Oxidative Injury
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