scholarly journals Spore Germination and Rhizoid Differentiation in Onoclea sensibilis

1984 ◽  
Vol 74 (3) ◽  
pp. 656-662 ◽  
Author(s):  
Clark S. Huckaby ◽  
John H. Miller
Keyword(s):  
Spore Germination ◽  
Onoclea Sensibilis

A heavy metal biossay based on percent spore germination of the sensitive fern,Onoclea sensibilis

1980 ◽  
Vol 24 (1) ◽  
pp. 489-495 ◽  
Author(s):  
Raymond L. Petersen ◽  
Damon Arnold ◽  
David G. Lynch ◽  
Steven A. Price
Keyword(s):  
Heavy Metal ◽  
Spore Germination ◽  
Onoclea Sensibilis

Effects of light, temperature and pH on spore germination and early gametophytic development of Alsophila metteniana

2009 ◽  
Vol 17 (2) ◽  
pp. 182 ◽  
Author(s):  
Du Honghong ◽  
Li Yang ◽  
Li Dong ◽  
Dai Shaojun ◽  
Jiang Chuangdao ◽  
...  
Keyword(s):  
Spore Germination

Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila

2019 ◽  
Vol 26 (6) ◽  
pp. 414-422
Author(s):  
Jia Liu ◽  
Ping Song ◽  
Jie Zhang ◽  
Ziyan Nangong ◽  
Xiaobei Liu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Insecticidal Activity ◽  
Spore Germination ◽  
Binding Protein ◽  
Colloidal Chitin ◽  
Inhibition Rate ◽  
Xenorhabdus Nematophila ◽  
Chitin Binding Protein ◽  
And Function

Background: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). Objective: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. Methods: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. Results: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. Conclusion: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

Sign in / Sign up

Export Citation Format

Share Document