scholarly journals Characterization of Radish (Raphanus sativus) Storage Proteins

1984 ◽  
Vol 74 (3) ◽  
pp. 487-493 ◽  
Author(s):  
Monique Laroche ◽  
Lorette Aspart ◽  
Michel Delseny ◽  
Paul Penon
2006 ◽  
Vol 54 (8) ◽  
pp. 3114-3120 ◽  
Author(s):  
Savithiry S. Natarajan ◽  
Chenping Xu ◽  
Hanhong Bae ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett

1979 ◽  
Vol 34 (9-10) ◽  
pp. 715-720 ◽  
Author(s):  
Gerhild Nurmann ◽  
Dieter Strack

Abstract From cotyledons of Raphanus sativus (red radish) an esterase activity which catalyzes the hy­drolysis of sinapine into sinapic acid and choline has been isolated. The enzyme, which has a near absolute specificity, is not analogous with any esterase described in the literature. The reaction has a pH optimum of 8.5 and the apparent Km is 1.95 × 10-5 m. The enzyme is relatively insensi­tive to both physostigmine (eserine) {Ki = 1.73 × 10-4 m) and neostigmine (Ki = 2 .1 3 × 10-4 ᴍ). Diisopropyl fluorophosphate (DFP) showed no inhibition and diethyl p-nitrophenylphosphate (E 600) only a slight inhibitory effect at 10-5 ᴍ, respectively. Choline (10-2 ᴍ) was inhibitory but acetylcholine (10-2 ᴍ) stimulated the enzyme activity.


2018 ◽  
Vol 125 (1) ◽  
pp. 12518
Author(s):  
Mamounata Diao ◽  
Crépin I. Dibala ◽  
Brice N’cho Ayékoué ◽  
Mamoudou H. Dicko

2003 ◽  
Vol 82 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Rajni Mujoo ◽  
Dianne T. Trinh ◽  
Perry K.W. Ng
Keyword(s):  

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