scholarly journals Evidence for Compartmentalization of Conjugates of 2,4-Dichlorophenoxyacetic Acid in Soybean Callus Tissue

1982 ◽  
Vol 70 (4) ◽  
pp. 939-942 ◽  
Author(s):  
Gayle H. Davidonis ◽  
Robert H. Hamilton ◽  
Ralph O. Mumma
Keyword(s):  
Callus Tissue ◽  
Dichlorophenoxyacetic Acid ◽  
Soybean Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Growth substance requirements and major lipid constituents of tissue cultures of Euphorbia esula and E. cyparissias

1972 ◽  
Vol 50 (4) ◽  
pp. 723-726 ◽  
Author(s):  
T. T. Lee ◽  
A. N. Starratt
Keyword(s):  
Callus Tissue ◽  
Defined Medium ◽  
Growth Substance ◽  
Tissue Growth ◽  
Euphorbia Esula ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Root Tissues

The root tissues of Euphorbia esula and E. cyparissias form callus on chemically defined medium. Both species require an exogenous supply of auxin for growth, but the appearance and color of the tissue and their responses to kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA) are different. The tissue growth is more satisfactory with α-naphthaleneacetic acid (NAA) than with 2,4-D, IAA, or 4-amino-3,5,6-trichloropicolinic acid (picloram). Gibberellic acid has no effect. The callus tissues of E. esula become intensely green under light but are not autotrophic.Triglycerides, palmitic acid, and β-sitosterol are the major lipid constituents of the callus tissue of E. esula. Chromatographic analysis reveals no significant differences in the composition of extracts from the non-green and green tissues. Long-chain aldehydes, alcohols, and triterpenes found in the plant are not detected in the cultures.

Induction and Biochemical Parameters of Callus Growth from Three Peanut Cultivars1

1978 ◽  
Vol 5 (2) ◽  
pp. 78-82 ◽  
Author(s):  
A. L. Guy ◽  
J. L. Heinis ◽  
S. K. Pancholy
Keyword(s):  
Amino Acids ◽  
Callus Tissue ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aromatic Amino Acids ◽  
Callus Growth ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Relative Growth Rates ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract The phytohormones 2,4-dichlorophenoxyacetic acid, naphthalene acetic acid and kinetin nave been employed to induce callus growth from the cotyledon tissue of three commercial peanut cultivars: ‘Early Bunch’ (EB), ‘NC-Fla 14’ (NC) and ‘Florunner’ (FR). Cultivar specific parameters have been examined for both cotyledon and callus tissue. The relative growth rates of callus tissues were always EB>NC>FR. SDS-polyacrylamide gel electrophoresis performed on the proteins from both types of tissue demonstrated mat most cultivar specific differences seen in cotyledon tissue are maintained by callus tissue from the same plant. However, more high molecular weight protein fractions were observed in cotyledon tissue than in callus. Amino acid analysis of these tissues revealed a higher concentration of neutral and aromatic amino acids in cotyledon while callus tissue was higher in basic amino acids.

scholarly journals Initiation and Maturation of Somatic Embryos of Squash (Cucurbito pepo)

HortScience ◽  
1992 ◽  
Vol 27 (1) ◽  
pp. 59-60 ◽  
Author(s):  
Paula P. Chee
Keyword(s):  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Cucurbita Pepo ◽  
Callus Tissue ◽  
Dichlorophenoxyacetic Acid ◽  
Summer Squash ◽  
Napthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Mature Seeds

Plant regeneration from tissue cultures of summer squash (Cucurbita pepo L. ev. YC60) has been observed. Embryogenic callus tissues were initiated when cotyledons of mature seeds were excised and cultured on Murashige and Skoog (MS) medium supplemented with either 22.7 μm 2,4-D or a combination of 4.7 μm 2,4,5-T, 4 μm BA, and 0.5 μm kinetin. Clusters of somatic embryos were found in callus tissue. Maturation of these somatic embryos was effected by transfer of embryogenic callus tissues to MS supplemented with 0.5 μm NAA and 0.25 μm kinetin. Regenerated mature plants were morphologically normal and set fruits containing seeds that germinated normally. Chemical names used: 6-benzylaminopurine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); α - napthaleneacetic acid (NAA); 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

Characterization of the Reproductive Mode in Guayule In Vitro

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 483a-483
Author(s):  
Roy N. Keys ◽  
Dennis T. Ray ◽  
David A. Dierig
Keyword(s):  
Field Experiments ◽  
Reproductive Mode ◽  
Dichlorophenoxyacetic Acid ◽  
Initial Field ◽  
Breeding Lines ◽  
Reproductive Modes ◽  
Desert Southwest ◽  
2,4 Dichlorophenoxyacetic Acid

Guayule (Parthenium argentatum Gray, Asteraceae) is a latex-producing perennial desert shrub that is potentially of economic importance as an industrial crop for the desert Southwest. It is known to possess complex reproductive modes. Diploids are predominantly sexual and self-incompatible, while polyploids show a range of apomictic potential and self-compatibility. This paper describes the development of a relatively rapid and simple technique for characterizing reproductive modes of breeding lines of P. argentatum. Initial field experiments were based on an auxin test used successfully to characterize reproductive mode in the Poaceae. The application of 2,4-dichlorophenoxyacetic acid inhibited embryo formation in P. argentatum, but this was not the case with other auxins tested. Results of field experiments were ambiguous because: 1) the floral structure of P. argentatum is such that auxins might not have penetrated to the ovules, and 2) there was potential self-fertilization by pollen released within isolation bags. Therefore, in vitro culture of flower heads was tested because it provided much better control of environmental conditions, growth regulator application, and pollen release. Auxin alone, or in combination with gibberellic acid or kinetin, inhibited parthenogenesis in vitro. Embryo production did not vary using two substantially different nutrient media. In vitro flower head culture using a (Nitsch and Nitsch) liquid nutrient medium without growth regulators, enabled characterization of the reproductive mode of seven breeding lines, ranging from predominantly sexual to predominantly apomictic. The results of this technique were substantiated using RAPD analyzes of progeny arrays from controlled crosses.

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