Metabolism of 2,4-dichlorophenoxyacetic acid. VII. Comparison of metabolites from five species of plant callus tissue cultures

1975 ◽  
Vol 23 (3) ◽  
pp. 373-376 ◽  
Author(s):  
Chao-Shieung. Feung ◽  
Robert H. Hamilton ◽  
Ralph O. Mumma
Keyword(s):  
Callus Tissue ◽  
Dichlorophenoxyacetic Acid ◽  
Tissue Cultures ◽  
2,4 Dichlorophenoxyacetic Acid

Growth substance requirements and major lipid constituents of tissue cultures of Euphorbia esula and E. cyparissias

1972 ◽  
Vol 50 (4) ◽  
pp. 723-726 ◽  
Author(s):  
T. T. Lee ◽  
A. N. Starratt
Keyword(s):  
Callus Tissue ◽  
Defined Medium ◽  
Growth Substance ◽  
Tissue Growth ◽  
Euphorbia Esula ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Root Tissues

The root tissues of Euphorbia esula and E. cyparissias form callus on chemically defined medium. Both species require an exogenous supply of auxin for growth, but the appearance and color of the tissue and their responses to kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA) are different. The tissue growth is more satisfactory with α-naphthaleneacetic acid (NAA) than with 2,4-D, IAA, or 4-amino-3,5,6-trichloropicolinic acid (picloram). Gibberellic acid has no effect. The callus tissues of E. esula become intensely green under light but are not autotrophic.Triglycerides, palmitic acid, and β-sitosterol are the major lipid constituents of the callus tissue of E. esula. Chromatographic analysis reveals no significant differences in the composition of extracts from the non-green and green tissues. Long-chain aldehydes, alcohols, and triterpenes found in the plant are not detected in the cultures.

scholarly journals Strategies for the Regeneration of Paphiopedilum callosum through Internode Tissue Cultures Using Dark–light Cycles

HortScience ◽  
2019 ◽  
Vol 54 (5) ◽  
pp. 920-925
Author(s):  
Nguyen Phuc Huy ◽  
Vu Quoc Luan ◽  
Le Kim Cuong ◽  
Nguyen Ba Nam ◽  
Hoang Thanh Tung ◽  
...  
Keyword(s):  
Stem Elongation ◽  
Gelling Agent ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Tissue Cultures ◽  
Organic Substances ◽  
Dark Light ◽  
Support Matrix ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Bacto Agar

Paphiopedilum spp. is one of the most commercially popular orchids because of its variety of shapes, sizes, and colors. However, it is at risk for extinction because of its exploitation. Regeneration of orchid plants using internode segments is extremely difficult. In this study, young P. callosum plants (1.5 cm) were exposed to eight dark–light cycles (14 days of dark and 1 day of light) for stem elongation to increase the number of nodes to obtain internode tissues. After 75 days of culture, the highest callogenesis (31.25%) was achieved when internode tissue was cultured on liquid Schenk and Hildebrandt (SH) medium containing 30 g·L−1 sucrose, 1.0 mg·L−1 Thidiazuron (TDZ), 1.0 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), and cotton wool as the support matrix. The optimal media for induction of protocorm-like bodies (PLBs) were the same compositions as previously mentioned and were supplemented with 9 g·L−1 Bacto agar as the gelling agent. PLB clumps (5–6 PLBs/clump) produced the best shoots on medium containing 0.5 mg·L−1 α-Naphthaleneacetic acid (NAA) and 0.3 mg·L−1 TDZ. Among the organic substances tested, 200 g·L−1 potato homogenate (PH) added to Hyponex N016 medium supplemented with 1.0 mg·L−1 NAA, 30 g·L−1 sucrose, 170 mg·L−1 NaH2PO4, 1.0 g·L−1 peptone, and 9 g·L−1 Bacto agar resulted in the best rooting. The rooted plantlets with four to five leaves were acclimatized and had a 100% survival rate. The method presented in this research provides a strategy for the development of highly effective propagation of Paphiopedilum species using ex vitro explants for both conservation and horticultural purposes.

Induction and Biochemical Parameters of Callus Growth from Three Peanut Cultivars1

1978 ◽  
Vol 5 (2) ◽  
pp. 78-82 ◽  
Author(s):  
A. L. Guy ◽  
J. L. Heinis ◽  
S. K. Pancholy
Keyword(s):  
Amino Acids ◽  
Callus Tissue ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aromatic Amino Acids ◽  
Callus Growth ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Relative Growth Rates ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract The phytohormones 2,4-dichlorophenoxyacetic acid, naphthalene acetic acid and kinetin nave been employed to induce callus growth from the cotyledon tissue of three commercial peanut cultivars: ‘Early Bunch’ (EB), ‘NC-Fla 14’ (NC) and ‘Florunner’ (FR). Cultivar specific parameters have been examined for both cotyledon and callus tissue. The relative growth rates of callus tissues were always EB>NC>FR. SDS-polyacrylamide gel electrophoresis performed on the proteins from both types of tissue demonstrated mat most cultivar specific differences seen in cotyledon tissue are maintained by callus tissue from the same plant. However, more high molecular weight protein fractions were observed in cotyledon tissue than in callus. Amino acid analysis of these tissues revealed a higher concentration of neutral and aromatic amino acids in cotyledon while callus tissue was higher in basic amino acids.

scholarly journals Initiation and Maturation of Somatic Embryos of Squash (Cucurbito pepo)

HortScience ◽  
1992 ◽  
Vol 27 (1) ◽  
pp. 59-60 ◽  
Author(s):  
Paula P. Chee
Keyword(s):  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Cucurbita Pepo ◽  
Callus Tissue ◽  
Dichlorophenoxyacetic Acid ◽  
Summer Squash ◽  
Napthaleneacetic Acid ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Mature Seeds

Plant regeneration from tissue cultures of summer squash (Cucurbita pepo L. ev. YC60) has been observed. Embryogenic callus tissues were initiated when cotyledons of mature seeds were excised and cultured on Murashige and Skoog (MS) medium supplemented with either 22.7 μm 2,4-D or a combination of 4.7 μm 2,4,5-T, 4 μm BA, and 0.5 μm kinetin. Clusters of somatic embryos were found in callus tissue. Maturation of these somatic embryos was effected by transfer of embryogenic callus tissues to MS supplemented with 0.5 μm NAA and 0.25 μm kinetin. Regenerated mature plants were morphologically normal and set fruits containing seeds that germinated normally. Chemical names used: 6-benzylaminopurine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); α - napthaleneacetic acid (NAA); 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).

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