scholarly journals A Rapid Method for the Extraction and Analysis of Abscisic Acid from Plant Tissue

1980 ◽  
Vol 65 (3) ◽  
pp. 523-525 ◽  
Author(s):  
Kerry T. Hubick ◽  
David M. Reid
Keyword(s):  
Abscisic Acid ◽  
Rapid Method ◽  
Plant Tissue

Choice of methods for the determination of abscisic acid in plant tissue

1983 ◽  
Vol 256 ◽  
pp. 439-446 ◽  
Author(s):  
E.B. Dumbroff ◽  
M.A. Walker ◽  
P.A. Dumbroff
Keyword(s):  
Abscisic Acid ◽  
Plant Tissue

scholarly journals A Rapid Method for Quantifying RNA and Phytohormones From a Small Amount of Plant Tissue

2020 ◽  
Vol 11 ◽  
Author(s):  
Da Cao ◽  
Francois Barbier ◽  
Kaori Yoneyama ◽  
Christine A. Beveridge
Keyword(s):  
Rapid Method ◽  
Plant Tissue

ABSCISIC ACID LEVELS AND GENETIC COMPACTION IN APPLE SEEDLINGS

1977 ◽  
Vol 57 (1) ◽  
pp. 81-85 ◽  
Author(s):  
J. M. LEE ◽  
N. E. LOONEY
Keyword(s):  
Abscisic Acid ◽  
Rapid Method ◽  
Growth Habit ◽  
Shoot Tip ◽  
Malus Sylvestris ◽  
Growth Types ◽  
Compact Growth ◽  
Controlled Crosses

Apple (Malus sylvestris Mill.) seedlings arising from controlled crosses involving McIntosh Wijcik segregate reliably into normal and compact growth types. The compact or spur-type seedlings have shorter, thicker stems; shorter internodes; and a strong upright, nonbranching growth habit. They contained less free ABA per shoot tip than normal seedlings. A reliable and rapid method for extracting and measuring ABA is described.

Inhibition by abscisic acid of the light and dark uptake of potassium by slices of Vicia faba leaves

1972 ◽  
Vol 50 (9) ◽  
pp. 1915-1917 ◽  
Author(s):  
Roger F. Horton ◽  
K. R. Bruce
Keyword(s):  
Abscisic Acid ◽  
Vicia Faba ◽  
Plant Tissue ◽  
Potassium Uptake ◽  
Mature Leaves ◽  
Ion Movement ◽  
The Media ◽  
Potassium Influx

Abscisic acid at a concentration of 10 mg/liter inhibits up to 79% of the potassium uptake of slices of expanding Vicia faba leaves in the dark, and up to 75% of the greater potassium influx occurring in the light. Abscisic acid has no effect on the potassium influx into tissue from non-expanding, mature leaves which consistently leak potassium into the media. The possible role of abscisic acid in ion movement within plant tissue is discussed.

Improved Extraction of Abscisic Acid From Plant Tissue

1988 ◽  
Vol 15 (3) ◽  
pp. 421 ◽  
Author(s):  
BR Loveys ◽  
HM van Dijk
Keyword(s):  
Abscisic Acid ◽  
Plant Tissue ◽  
Room Temperature ◽  
Internal Standard ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Tissues ◽  
Enzymic Hydrolysis ◽  
Solvent Residues ◽  
Hydrolysis Of ◽  
Extracting Solvent

Methods have been developed for the extraction of abscisic acid (ABA) from plant tissues using water as the extracting solvent. Abnormally high levels of ABA were extracted with water at room temperature from some tissues. This was attributed to enzymic hydrolysis of conjugated forms of ABA. This problem was overcome by briefly boiling the plant tissue in water before homogenisation. Subsequent analysis of ABA was by combined gas chromatography/mass spectrometry using deuterium-labelled ABA as an internal standard but it is suggested that the method may be particularly suited to assay procedures based on immunological techniques where solvent residues may be undesirable.

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