scholarly journals Kinetin Incorporated into Tobacco Callus Ribosomal RNA and Transfer RNA Preparations

1977 ◽  
Vol 60 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Norimoto Murai ◽  
Barbara J. Taller ◽  
Donald J. Armstrong ◽  
Folke Skoog ◽  
Melvin A. Micke ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Transfer Rna ◽  
Tobacco Callus

scholarly journals The complete mitochondrial genome and description of a new cryptic species of Benedenia Diesing, 1858 (Monogenea: Capsalidae), a major pathogen infecting the yellowtail kingfish Seriola lalandi Valenciennes in the South-East Pacific

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
J. Antonio Baeza ◽  
Fabiola A. Sepúlveda ◽  
M. Teresa González
Keyword(s):  
Mitochondrial Genome ◽  
Ribosomal Rna ◽  
Complete Mitochondrial Genome ◽  
Transfer Rna ◽  
The South ◽  
Protein Coding ◽  
East Pacific ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi ◽  
Rna Genes

Abstract Background The monogenean Benedenia seriolae parasitizes fishes belonging to the genus Seriola, represents a species complex, and causes substantial impact on fish welfare in aquaculture systems worldwide. This study reports, for the first time, the complete mitochondrial genome of B. humboldti n. sp., a new cryptic species from the South-East Pacific (SEP). Methods The mitogenome of B. humboldti n. sp. was assembled from short Illumina 150 bp pair-end reads. The phylogenetic position of B. humboldti n. sp. among other closely related congeneric and confamiliar capsalids was examined using mitochondrial protein-coding genes (PCGs). Morphology of B. humboldti n. sp. was examined based on fixed and stained specimens. Results The AT-rich mitochondrial genome of B. humboldti is 13,455 bp in length and comprises 12 PCGs (atp8 was absent as in other monogenean genomes), 2 ribosomal RNA genes, and 22 transfer RNA genes. All protein-coding, ribosomal RNA, and transfer RNA genes are encoded on the H-strand. The gene order observed in the mitochondrial genome of B. humboldti n. sp. was identical to that of B. seriolae from Japan but different from that of B. seriolae from Australia. The genetic distance between B. humboldti n. sp. and B. seriolae from Japan was high. Minor but reliable differences in the shape of the penis were observed between Benedenia humboldti n. sp. and congeneric species. Conclusions Phylogenetic analyses based on PCGs in association with differences in the shape of the penis permitted us to conclude that the material from the South-East Pacific represents a new species of Benedenia infecting S. lalandi off the coast of Chile. The discovery of this parasite represents the first step to improving our understanding of infestation dynamics and to develop control strategies for this pathogen infecting the farmed yellowtail kingfish, Seriola lalandi, in the South-East Pacific.

Regions of 23 S Ribosomal RNA Proximal to Transfer RNA bound at the P and E Sites

1995 ◽  
Vol 252 (5) ◽  
pp. 572-582 ◽  
Author(s):  
James M. Bullard ◽  
Michael A. van Waes ◽  
Douglas J. Bucklin ◽  
Walter E. Hill
Keyword(s):  
Ribosomal Rna ◽  
Transfer Rna

Nuclear domains of the RNA subunit of RNase P

1997 ◽  
Vol 110 (7) ◽  
pp. 829-837
Author(s):  
M.R. Jacobson ◽  
L.G. Cao ◽  
K. Taneja ◽  
R.H. Singer ◽  
Y.L. Wang ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Mammalian Cells ◽  
Rat Kidney ◽  
Rnase P ◽  
Transfer Rna ◽  
Binding Domain ◽  
Ribosomal Rna Processing ◽  
Nuclear Domains ◽  
Fibrillar Component

The ribonucleoprotein enzyme RNase P catalyzes the 5′ processing of pre-transfer RNA, and has also recently been implicated in pre-ribosomal RNA processing. In the present investigation, in situ hybridization revealed that RNase P RNA is present throughout the nucleus of mammalian cells. However, rhodamine-labeled human RNase P RNA microinjected into the nucleus of rat kidney (NRK) epithelial cells or human (HeLa) cells initially localized in nucleoli, and subsequently became more evenly distributed throughout the nucleus, similar to the steadystate distribution of endogenous RNase P RNA. Parallel microinjection and immunocytochemical experiments revealed that initially nucleus-microinjected RNase P RNA localized specifically in the dense fibrillar component of the nucleolus, the site of pre-rRNA processing. A mutant RNase P RNA lacking the To antigen binding domain (nucleotides 25–75) did not localize in nucleoli after nuclear microinjection. In contrast, a truncated RNase P RNA containing the To binding domain but lacking nucleotides 89–341 became rapidly localized in nucleoli following nuclear microinjection. However, unlike the full-length RNase P RNA, this 3′ truncated RNA remained stably associated with the nucleoli and did not translocate to the nucleoplasm. These results suggest a nucleolar phase in the maturation, ribonucleoprotein assembly or function of RNase P RNA, mediated at least in part by the nucleolar To antigen. These and other recent findings raise the intriguing possibility of a bifunctional role of RNase P in the nucleus: catalyzing pre-ribosomal RNA processing in the nucleolus and pre-transfer RNA processing in the nucleoplasm.

Nucleotide sequences of small ribosomal RNA and adjacent transfer RNA genes in rat mitochondrial DNA

Gene ◽  
1981 ◽  
Vol 16 (1-3) ◽  
pp. 297-307 ◽  
Author(s):  
Kobayashi Midori ◽  
Seki Tetsunori ◽  
Yaginuma Katsuyuki ◽  
Koike Katsuro
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Transfer Rna ◽  
Nucleotide Sequences ◽  
Rna Genes
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