scholarly journals Effect of Gibberellic Acid, Kinetin, and Ethylene plus Carbon Dioxide on the Thermodormancy of Lettuce Seed (Lactuca sativa L. cv. Mesa 659)

1975 ◽  
Vol 56 (6) ◽  
pp. 826-829 ◽  
Author(s):  
Robert D. Keys ◽  
Orrin E. Smith ◽  
Junji Kumamoto ◽  
Jessye L. Lyon
Keyword(s):  
Carbon Dioxide ◽  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Lactuca Sativa L

The effects of ultra-high diluted gibberellic acid on in vitro lettuce seed germination.

2021 ◽  
Vol 15 (4) ◽  
pp. 50-54
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Germination Rate ◽  
Petri Dish ◽  
New Drugs ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Petri Dishes

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

scholarly journals EFEITO DO CO2 APLICADO VIA ÁGUA DE IRRIGAÇÃO SOBRE A CULTURA DA ALFACE (Lactuca sativa L.)

Irriga ◽  
2007 ◽  
Vol 12 (4) ◽  
pp. 492-504
Author(s):  
Jorge Tomoyoshi Tamagi ◽  
Márcio Antonio Vilas Boas ◽  
Silvio César Sampaio ◽  
Simone Damasceno ◽  
Vandeir Francisco Guimarães
Keyword(s):  
Carbon Dioxide ◽  
Experimental Design ◽  
Lactuca Sativa ◽  
Irrigation Water ◽  
Drip Irrigation ◽  
Control Plant ◽  
Study Objective ◽  
Economical Analysis ◽  
Lactuca Sativa L ◽  
Final Harvest

EFEITO DO CO2 APLICADO VIA ÁGUA DE IRRIGAÇÃO SOBRE A CULTURA DA ALFACE (Lactuca sativa L.)  Jorge Tomoyoshi Tamagi1; Marcio Antonio Vilas Boas1; Silvio Cesar Sampaio1; Simone Damasceno1; Vandeir Francisco Guimarães21Centro de Ciências Exatas e Tecnológicas-CCET, Universidade Estadual do Oeste do Paraná - Unioeste, Campus de Cascavel, Cascavel,  PR, [email protected] de Ciências Agrárias-CCA, Universidade Estadual do Oeste do Paraná – Unioeste, Campus de Marechal Cândido Rondon, Marechal Cândido Rondon,  PR   1 RESUMO                     Este trabalho teve como objetivo estudar a influência de diferentes doses de aplicação de CO2, via água de irrigação por gotejamento com o uso de fitas Poritex,  no desenvolvimento e na produtividade da alface (Lactuca sativa L.). O experimento foi realizado no período de março à maio de 2004, em Cascavel-PR. O experimento foi conduzido sob túneis plásticos em delineamento experimental inteiramente casualizado, representado por quatro tratamentos com cinco repetições com a finalidade de testar as doses de CO2  na alface, cultivar Vera. As doses de CO2 foram  0, 1, 3 e 6 L min-1, sendo as irrigações  realizadas diariamente às 10h, durante 10 minutos, após 10 dias do transplante das mudas até 1 dia antes da colheita final, totalizando 215 mm de água por canteiro. Os resultados mostraram que as doses 1 e 3 L min-1 de CO2 aplicadas via água promoveram aumento na produtividade, atingindo valores de 26,52% e 14,47%, quando comparado à testemunha. A análise econômica mostrou viabilidade da aplicação de CO2 via água de irrigação. UNITERMOS: irrigação por gotejamento; dióxido de carbono; alface; doses.  TAMAGI, J.T.; VILAS BOAS, M.A.; SAMPAIO, S.C.; DAMASCENO, S.; GUIMARÃES, V.F. EFFECT OF CO2 APPLIED TO LETTUCE CROP (Lactuca sativa L.) THROUGH IRRIGATION WATER  2 ABSTRACT                     This study objective was to study the influence of different rates of CO2 application, by  irrigation water, by dripping it with the use of poritex bands, on lettuce (Lactuca sativa L.) cultivation and production. The experiment was carried out from March to May, 2004, in Cascavel-PR. The experiment was conducted in plastic tunnels and ina random experimental design with four treatments and five replications in order to test predetermined CO2 rates on lettuce, Vera cultivar. The CO2 rates were 0, 1, 3 and 6 L min-1. The irrigation was daily at 10 o’clock during 10 minutes, 10 days after the plants transplantation, until 01 day before the final harvest, adding up to 215 mm of the water per portion. The results have shown that the 1 and 3 L min-1 CO2 rates, applied by water, caused increase in production, obtaining 26.52% and 14.47%  values,  when  compared  to  the control  plant.  The economical analysis indicated the viability of CO2 application via irrigation water.KEYWORDS: drip irrigation; carbon dioxide; lettuce; rates

Gibberellic acid induced elongation of lettuce hypocotyls and its inhibition by colchicine

1974 ◽  
Vol 52 (1) ◽  
pp. 259-264 ◽  
Author(s):  
V. K. Sawhney ◽  
L. M. Srivastava
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Kinetic Data ◽  
Maximum Elongation ◽  
Short Term ◽  
Ultrastructural Studies ◽  
Lactuca Sativa L ◽  
Hypocotyl Development

Lettuce (Lactuca sativa L.) seedlings treated with gibberellic acid (GA) for 72 h produced hypocotyls 600% longer than the water controls. The process of elongation was accelerated by GA. Also, for maximum elongation, presence of GA was not necessary after 20 h. Short term treatments of seedlings with GA indicated that the seedlings were most 'sensitive' to GA after 8 h of growth in water. Colchicine inhibited the GA-induced elongation. Its effect was maximal if it was applied within the first 2 h after GA treatment irrespective of the stage of hypocotyl development; the inhibition was progressively lessened after 2 h and lost after 36 h. These kinetic data are presented together with measurements of hypocotyl cell lengths and widths and provide a basis for correlated ultrastructural studies.

scholarly journals Distinct Metabolome Changes during Seed Germination of Lettuce (Lactuca sativa L.) in Response to Thermal Stress as Revealed by Untargeted Metabolomics Analysis

2020 ◽  
Vol 21 (4) ◽  
pp. 1481 ◽  
Author(s):  
Shiwei Wei ◽  
Xiao Yang ◽  
Guotao Huo ◽  
Guojun Ge ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Thermal Stress ◽  
Seed Germination ◽  
Organic Acids ◽  
Lactuca Sativa ◽  
Sesquiterpene Lactones ◽  
Germination Percentage ◽  
Untargeted Metabolomics ◽  
Lettuce Seed ◽  
Lactuca Sativa L

Temperature strongly influences lettuce (Lactuca sativa L.) seed germination. Different lettuce genotypes respond differently to higher temperatures or thermal stress. In this study, we evaluated the germination performance of 304 lettuce accessions incubated at three temperature settings, 21 °C, 28 °C and 35 °C, respectively, for 40 h. At 21 °C, seeds of all 304 accessions germinated with very well an average germination percentage of 87.72%; at 28 °C, the average germination percentage dropped to 42.84% and at 35 °C, the germination decreased to 1.01%. Then, we investigated changes in metabolic profiles of lettuce seed response to thermal stress using an untargeted metabolomics approach. Results suggested that seeds of thermal-sensitive and thermal-tolerant cultivars employed different metabolic strategies in response to thermal stress during germination. Thermal-sensitive buds accumulated more significant amounts of organic acids, amino acids, sugars, sterols, phenolic compounds and terpenoids compared to thermal-tolerant buds at 21 °C. Thermal-tolerant lettuce cultivar accumulated higher concentrations of amino acids, organic acids, sugars, sesquiterpene lactones, sterols, and fatty acids derivatives during the germination at 35 °C compared to germinated at 21 °C. This investigation paves the way to link the metabolomics to other external and internal factors affecting lettuce seed germination under thermal stress.

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