scholarly journals Survival of Plant Tissue at Super-Low Temperatures. IV. Cell Survival with Rapid Cooling and Rewarming

1966 ◽  
Vol 41 (6) ◽  
pp. 1050-1054 ◽  
Author(s):  
A. Sakai
Keyword(s):  
Cell Survival ◽  
Plant Tissue ◽  
Low Temperatures ◽  
Rapid Cooling

γ-U phase in U-Pt system retained to low temperatures by means of rapid cooling

2016 ◽  
Vol 479 ◽  
pp. 287-294 ◽  
Author(s):  
N.-T.H. Kim-Ngan ◽  
M. Paukov ◽  
R. Tarasenko ◽  
V. Tkáč ◽  
P. Minarik ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Rapid Cooling

Some Properties of Two Vulcanized Pure Gum Compounds at Low Temperatures

1939 ◽  
Vol 12 (2) ◽  
pp. 370-380
Author(s):  
A. A. Somerville
Keyword(s):  
Tensile Strength ◽  
Low Temperatures ◽  
Rapid Cooling

Abstract (1) High and normal sulfur pure gum compounds became board-like and brittle within a few minutes when cooled at −60° C, yet when tested at this temperature they had an elongation of over 500% and tensile strength of over 5,000 lbs. per sq. in. (2) With decreasing temperature the two compounds gave increasing tensile strength and modulus and decreasing elongation. (3) Relatively high combined sulfur, i. e., up to over 8% on the rubber, was accompanied by a marked increase in hardness, even at temperatures no lower than −30° C. (4) A substitution of deproteinized rubber for smoked sheet appeared to give greater resistance to hardening at temperatures down to −40° C. (5) Unvulcanized, unmilled smoked sheet showed greater resistance to hardening on rapid cooling than the vulcanized compounds at all temperatures tested. This phenomenon, however, is not to be confused with that of ordinary frozen rubber, which is commonly observed at temperatures above −30° C.

Mechanism of survival in plant cells at super-low temperatures by rapid cooling and rewarming

Cryobiology ◽  
1968 ◽  
Vol 4 (4) ◽  
pp. 165-173 ◽  
Author(s):  
A. Sakai ◽  
K. Ötsuka ◽  
S. Yoshida
Keyword(s):  
Low Temperatures ◽  
Plant Cells ◽  
Rapid Cooling

Quenched superconductivity by rapid cooling down to low temperatures below Tc2 in single-crystal HoMo6S8

1986 ◽  
Vol 60 (10) ◽  
pp. 771-775 ◽  
Author(s):  
Y. Koike ◽  
T. Fukase ◽  
N. Kobayashi ◽  
S. Hosoya ◽  
H. Takei
Keyword(s):  
Single Crystal ◽  
Low Temperatures ◽  
Rapid Cooling

Freezing and Freeze-Drying of Bull Spermatozoa

1957 ◽  
Vol 190 (2) ◽  
pp. 281-286 ◽  
Author(s):  
J. K. Sherman
Keyword(s):  
Cause Of Death ◽  
Low Temperatures ◽  
Freeze Drying ◽  
Ice Formation ◽  
Physical Injury ◽  
Freezing And Thawing ◽  
Rapid Cooling ◽  
Glass Slides ◽  
Presence And Absence

The possible dependance of survival upon the time spermatozoa are in contact with the protective substance glycerol, prior to exposure to low temperatures, was investigated. Pretreatment for 5 minutes provided at least as much protection as 18 hours. Microscopic observations on ice formation relative to survival in semen frozen by four different methods, in the presence and absence of glycerol, gave no evidence for the notion that physical injury by ice is the cause of death on freezing and thawing. Site and character of ice formed were preserved by freeze-drying. A study on the effect of rates of cooling on survival revealed that the theory of survival based upon vitrification by rapid cooling does not apply to bull spermatozoa. Spermatozoa in smears of semen on glass slides did not withstand the process of freeze-drying for various periods. Death was attributed to drying and/or reconstitution.

scholarly journals Detection of Colletotrichum acutatum latent infections in strawberry petioles and leaves

2008 ◽  
Vol 23 (4) ◽  
pp. 235-241
Author(s):  
Natasa Duduk ◽  
Mirko Ivanovic ◽  
Bojan Duduk
Keyword(s):  
Plant Tissue ◽  
Low Temperatures ◽  
Plant Material ◽  
Fruit Rot ◽  
Colletotrichum Acutatum ◽  
Tween 20 ◽  
Surface Sterilization ◽  
Plant Parts ◽  
Symptomless Plant ◽  
Freezing Procedure

Colletotrichum acutatum is the most significant agent of anthracnose strawberry fruit rot. Besides being a necrotrophic pest, it can spend a part of its life cycle as an epiphyte, in a form of latent infection. The presence of the fungi on symptomless plant tissue is considered one of the main ways of distribution of this economically harmful pathogen in the world. Investigation of latent C. acutatum infection was carried out on artificially inoculated strawberries. The initiation of fungi sporulation on symptomless petioles and leaves was carried out by exposing them to the herbicide paraquat (0.25%) and low temperatures, which caused plant tissue decay in different ways. Surface sterilization with 0.5% NaOCl precedes the exposure of plant material to paraquat. The freezing procedure was carried out by exposure of plant material to the temperature of -20?C for 2h. After the freezing, one group was rinsed in Tween 20 (18 ?l/l), and another group underwent surface sterilization in 0.0525% NaOCl with an addition of Tween 20 (18 ?l/l). After 6 days of incubation, the appearance of acervuli and conidia was detected in 93.33 to 100% plant parts exposed to paraquat treatment and freezing procedure. In inoculated parts which were not exposed to herbicides or low temperatures, the presence of acervuli was detected in 3.33% tested petioles and 6.67% leaves.

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