scholarly journals Effect of Gibberellic Acid and Photoperiod on Indoleacetic Acid Oxidase in Lupinus Albus L.

1960 ◽  
Vol 35 (3) ◽  
pp. 359-361 ◽  
Author(s):  
Ronald Watanabe ◽  
Robert E. Stutz
Keyword(s):  
Gibberellic Acid ◽  
Indoleacetic Acid ◽  
Lupinus Albus ◽  
Acid Oxidase ◽  
Indoleacetic Acid Oxidase ◽  
Lupinus Albus L

Increase of indoleacetic acid oxidase isoenzymes by gibberellic acid in tobacco callus cultures

1971 ◽  
Vol 49 (5) ◽  
pp. 687-693 ◽  
Author(s):  
T. T. Lee
Keyword(s):  
Gibberellic Acid ◽  
Indoleacetic Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Callus Cultures ◽  
Tobacco Callus ◽  
Acid Oxidase ◽  
Iaa Oxidase ◽  
Characteristic Type ◽  
Indoleacetic Acid Oxidase ◽  
Callus Tissues

Indoleacetic acid (IAA) oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) grown on a medium supplemented with gibberellic acid (GA3), IAA, and kinetin was resolved into seven isoenzymes by polyacrylamide gel electrophoresis. Gibberellic acid promoted the development of three fast-migrating IAA oxidase isoenzymes which were 7- to 10-fold more active than those of the control. Gibberellic acid was effective at 2 × 10−9 M and its activity increased with increasing concentration up to 2 × 10−7 M. Actinomycin D and cycloheximide inhibited the development of these isoenzymes, suggesting a requirement for both RNA and protein synthesis. The GA3-promoted IAA oxidase isoenzymes increased with time and were positively associated with the GA3-induced characteristic type of growth.The expression of the GA3 effect on IAA oxidase isoenzymes and growth was dependent on IAA and kinetin. In the presence of appropriate concentrations of kinetin, the combined effect of GA3 and IAA was more than additive. A possible interpretation of the GA3–IAA interaction is discussed.

scholarly journals Effect of Manganese Toxicity on the Indoleacetic Acid Oxidase System of Cotton

1966 ◽  
Vol 41 (4) ◽  
pp. 718-724 ◽  
Author(s):  
Page W. Morgan ◽  
Howard E. Joham ◽  
J. V. Amin
Keyword(s):  
Indoleacetic Acid ◽  
Manganese Toxicity ◽  
Acid Oxidase ◽  
Oxidase System ◽  
Indoleacetic Acid Oxidase

Studies of a Quinyl-p-coumarate in the Pineapple Plant (Ananas comosus var. cayenne)

1959 ◽  
Vol 12 (2) ◽  
pp. 240 ◽  
Author(s):  
GK Sutherland ◽  
WA Gortner
Keyword(s):  
Indoleacetic Acid ◽  
Spectral Characteristics ◽  
Periodate Oxidation ◽  
Quinic Acid ◽  
Ananas Comosus ◽  
Acid Oxidase ◽  
Coumaric Acid ◽  
Indoleacetic Acid Oxidase ◽  
Acid Lactone ◽  
Mild Hydrolysis

An ester is found in small concentrations in vegetative pineapple plants, with spectral characteristics in the ultraviolet of an ester of p-coumaric acid. p-Coumaric acid is obtained after hydrolysis, and the remaining aqueous hydrolysate indicates the presence of quinic acid lactone on chromatograms. On the basis of neutral equivalent determinations, boric acid conductivity and periodate oxidation experiments, and analyses following mild hydrolysis, the structure of the ester is suggested to be a quinyl-di-p-coumarate. It serves in the plant as a cofactor for pineapple indoleacetic acid oxidase.

scholarly journals Dehydration of Macromolecules I. Effect of Dehydration-Rehydration on Indoleacetic Acid Oxidase, Ribonuclease, Ribulosediphosphate Carboxylase, and Ketose-1-Phosphate Aldolase

1973 ◽  
Vol 26 (3) ◽  
pp. 591 ◽  
Author(s):  
B Darbyshire ◽  
BT Steer
Keyword(s):  
Water Potential ◽  
Indoleacetic Acid ◽  
Acid Oxidase ◽  
Enzyme Preparations ◽  
Water Potentials ◽  
Membrane Technique ◽  
Indoleacetic Acid Oxidase

A pressure-membrane technique has been developed to physically manipulate the water potential of in vitro enzyme preparations. Enzyme preparations were subjected to a range of water potentials using this technique.

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