scholarly journals Effect of Manganese Toxicity on the Indoleacetic Acid Oxidase System of Cotton

1966 ◽  
Vol 41 (4) ◽  
pp. 718-724 ◽  
Author(s):  
Page W. Morgan ◽  
Howard E. Joham ◽  
J. V. Amin
1960 ◽  
Vol 38 (5) ◽  
pp. 761-767 ◽  
Author(s):  
Ann Oaks ◽  
Michael Shaw

Flax rust mycelium was grown on cotyledons of flax in a modified Knop's medium. The mycelium produced and apparently secreted a typical peroxidase-mediated indoleacetic acid 'oxidase'. Decarboxylation of radioactive indoleacetate (—C14OOH) was stimulated by Mn++ (10−4 and 10−3M), dichlorophenol (10−4M), and resorcinol (10−4M). Catechol (10−4M) and pyrogallol (10−4M) inhibited the reaction. Moreover, pyrogallol competitively inhibited the reaction in the presence of resorcinol. All four phenols increased oxygen uptake by the mycelium, catechol and pyrogallol being the most effective. Cu++ (10−3M) inhibited the reaction by 60%; cyanide (10−3M) and diethydithiocarbamate (2 × 10−4M) were almost completely inhibitory. The results are discussed briefly in relation to host–parasite interactions and the control of indoleacetic acid oxidase activity in vivo.


1959 ◽  
Vol 12 (2) ◽  
pp. 240 ◽  
Author(s):  
GK Sutherland ◽  
WA Gortner

An ester is found in small concentrations in vegetative pineapple plants, with spectral characteristics in the ultraviolet of an ester of p-coumaric acid. p-Coumaric acid is obtained after hydrolysis, and the remaining aqueous hydrolysate indicates the presence of quinic acid lactone on chromatograms. On the basis of neutral equivalent determinations, boric acid conductivity and periodate oxidation experiments, and analyses following mild hydrolysis, the structure of the ester is suggested to be a quinyl-di-p-coumarate. It serves in the plant as a cofactor for pineapple indoleacetic acid oxidase.


1973 ◽  
Vol 26 (3) ◽  
pp. 591 ◽  
Author(s):  
B Darbyshire ◽  
BT Steer

A pressure-membrane technique has been developed to physically manipulate the water potential of in vitro enzyme preparations. Enzyme preparations were subjected to a range of water potentials using this technique.


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