scholarly journals Curvatures and Malformations in Bean Plants Caused by Culture Filtrate of Aspergillus niger.

1958 ◽  
Vol 33 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Roy W. Curtis
Water ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 119
Author(s):  
Suja Subhash ◽  
Pradeesh Babu ◽  
Amrutha Vijayakumar ◽  
Reshma Alookaran Suresh ◽  
Ajith Madhavan ◽  
...  

Robust control of pathogens in sewage facilitates safe reuse of wastewater rich in valuable nutrients for potential valorization through biological means. Aspergillus niger is widely reported in bioremediation of wastewater but studies on control of enteric pathogens in sewage are very sparse. So, this study aimed at exploring the antibacterial and nematicidal activity of A. niger culture filtrate (ACF). Antibacterial activity of ACF on enteric pathogens (Klebsiella pneumoniae, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella enterica, Shigella dysenteriae, Escherichia coli, Staphylococcus aureus, Klebsiella variicola) was determined by spectrophotometric growth analysis, resazurin based viability assay and biofilm formation assay. ACF showed inhibition against all enteric pathogens except Pseudomonas aeruginosa. Nematicidal studies on Caenorhabditis elegans showed 85% egg hatch inhibition and 52% mortality of L1 larvae. Sewage treatment with ACF at 1:1 (v/v) showed 2–3 log reduction in coliforms, Klebsiella, Shigella, Salmonella, S. aureus and Vibrio except Pseudomonas, indicating significant alteration of complex microbial dynamics in wastewater. Application of ACF can potentially be used as a robust biocontrol strategy against infectious microbes in wastewater and subsequent valorization by cultivating beneficial Pseudomonas.


HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 523f-523
Author(s):  
Russell Pressey ◽  
Richard B. Russell

Polygalacturonase inhibitors have been reported in a number of dicotyledonous plant tissues including pear and raspberry fruits and bean seedlings. These proteins inhibit fungal polygalacturonases and thus have been implicated in disease resistance in plants. The earlier work on the inhibitor from bean plants was conducted with hypocotyls as the source. We have found that immature bean pods contain much more inhibitor than other parts of the plant and developed a procedure for purification of this inhibitor. Fresh bean pods were extracted with 1.0 M NaCl at pH 7 and the proteins were precipitated with ammonium sulfate. The proteins were dissolved, dialyzed and chromatographed on a column of S-Sepharose. The inhibitor from this step was then chromatographed on a Mono Q column at high pH. Yields of the inhibitor varied somewhat with bean cultivar and pod maturity but were about ten times higher than from hypocotyls. The purified inhibitor reacted optimally with Aspergillus niger endopolygalacturonase at pH 4.3 and appeared to be similar to the inhibitor from hypocotyls. Bean pods thus are a convenient source of polygalacturonase inhibitor for studies on fruit maturation and disease resistance in plants.


2013 ◽  
Vol 170 (7) ◽  
pp. 1547-1559 ◽  
Author(s):  
Supratim Biswas ◽  
Rajib Dey ◽  
Siddhartha Mukherjee ◽  
Pataki C. Banerjee

2020 ◽  
Vol 21 (4) ◽  
pp. 839-850
Author(s):  
Xénia Pálfi ◽  
Zoltán Karácsony ◽  
Anett Csikós ◽  
Ottó Bencsik ◽  
András Szekeres ◽  
...  

2013 ◽  
Vol 47 (2) ◽  
pp. 63-71 ◽  
Author(s):  
Elżbieta Kuźniak ◽  
Henryk Urbanek ◽  
Aneta Michalak ◽  
Katarzyna Herka

The activity of ß-1,3-glucanase and chitinase in bean plants treated with <i>B. cinerea</i> products or/and infected and in cell cultures after application of fungal products has been studied. <i>Botrytis cinerea</i> infection and culture filtrates, ethanol precipitates, glucan and conidial extract treatment markedly enhanced the activity of both hydrolases. Cell cultures treated with <i>B.cinerea</i> products reacted similarly to intact plants. In plants pretreated with 2-day culture filtrate and conidial extract and then infected, ß-1,3-glucanase and chitinase were induced stronger than after infection without pretreatment.


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