scholarly journals Restriction Release: Improved Maize Transformation Efficiency

2019 ◽  
Vol 181 (4) ◽  
pp. 1397-1397
Author(s):  
Magdalena M. Julkowska
Keyword(s):  
Transformation Efficiency ◽  
Maize Transformation

Enhancement of maize transformation efficiency by the use of maize matrix attachment regions

2005 ◽  
Vol 80 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Fran�ois Torney ◽  
Anne Partier ◽  
Pierre Barret ◽  
Michel Beckert
Keyword(s):  
Transformation Efficiency ◽  
Matrix Attachment Regions ◽  
Maize Transformation

scholarly journals Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

2021 ◽  
Vol 7 (7) ◽  
pp. 520
Author(s):  
Jianmin Fu ◽  
Nohelli E. Brockman ◽  
Brian L. Wickes
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Yeast Cell ◽  
Transformation Efficiency ◽  
Transformation Frequency ◽  
Yeast Cells ◽  
Agar Concentration ◽  
Genetic Tool ◽  
Number Of Factors ◽  
Cryptococcus Spp ◽  
Transformation Systems

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.

scholarly journals WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 715
Author(s):  
Aline Kadri ◽  
Ghislaine Grenier De March ◽  
François Guerineau ◽  
Viviane Cosson ◽  
Pascal Ratet
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Truncatula ◽  
Transformation Efficiency ◽  
Genetic Manipulation ◽  
Leaf Explants ◽  
Promising Tool ◽  
Limiting Step ◽  
Wus Gene ◽  
Multiple Signaling ◽  
Regeneration Systems

The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryogenesis, we have investigated the effect of the heterologous ArabidopsisWUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants. WUS expression in leaf explants increased callogenesis and embryogenesis in the absence of growth regulators. Similarly, WUS expression enhanced the embryogenic potential of hairy root fragments. The WUS gene represents thus a promising tool to develop plant growth regulator-free regeneration systems or to improve regeneration and transformation efficiency in recalcitrant crops.

Maize transformation: history, progress, and perspectives

2021 ◽  
Vol 41 (6) ◽  
Author(s):  
Albert P. Kausch ◽  
Kan Wang ◽  
Heidi F. Kaeppler ◽  
William Gordon-Kamm
Keyword(s):  
Maize Transformation

scholarly journals Maize transformation technology development for commercial event generation

2014 ◽  
Vol 5 ◽  
Author(s):  
Qiudeng Que ◽  
Sivamani Elumalai ◽  
Xianggan Li ◽  
Heng Zhong ◽  
Samson Nalapalli ◽  
...  
Keyword(s):  
Technology Development ◽  
Maize Transformation ◽  
Event Generation

scholarly journals Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 421-430 ◽  
Author(s):  
A Pellegrineschi ◽  
L M Noguera ◽  
B Skovmand ◽  
R M Brito ◽  
L Velazquez ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Transformation Process ◽  
Plant Genotype ◽  
Donor Plant ◽  
Genotype Effect ◽  
Factors Influencing ◽  
Number Of Factors ◽  
Selection Medium ◽  
Callus Regeneration ◽  
Transformation Systems

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.Key words: wheat transformation, 'Bobwhite', genotype effect.

Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species

2018 ◽  
Vol 98 ◽  
pp. 16-24 ◽  
Author(s):  
Kolja N. Eckermann ◽  
Hassan M.M. Ahmed ◽  
Mohammad KaramiNejadRanjbar ◽  
Stefan Dippel ◽  
Christian E. Ogaugwu ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Insect Species

Comparison of in vitro transformation efficiency methods for Plasmodium falciparum

2021 ◽  
pp. 111432
Author(s):  
Siqi Wang ◽  
Weilin Zeng ◽  
Wei Zhao ◽  
Zheng Xiang ◽  
Hui Zhao ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Transformation Efficiency ◽  
In Vitro Transformation
Sign in / Sign up

Export Citation Format

Share Document