scholarly journals Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

2021 ◽  
Vol 7 (7) ◽  
pp. 520
Author(s):  
Jianmin Fu ◽  
Nohelli E. Brockman ◽  
Brian L. Wickes
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Yeast Cell ◽  
Transformation Efficiency ◽  
Transformation Frequency ◽  
Yeast Cells ◽  
Agar Concentration ◽  
Genetic Tool ◽  
Number Of Factors ◽  
Cryptococcus Spp ◽  
Transformation Systems

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.

scholarly journals Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 421-430 ◽  
Author(s):  
A Pellegrineschi ◽  
L M Noguera ◽  
B Skovmand ◽  
R M Brito ◽  
L Velazquez ◽  
...  
Keyword(s):  
Transformation Efficiency ◽  
Transformation Process ◽  
Plant Genotype ◽  
Donor Plant ◽  
Genotype Effect ◽  
Factors Influencing ◽  
Number Of Factors ◽  
Selection Medium ◽  
Callus Regeneration ◽  
Transformation Systems

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.Key words: wheat transformation, 'Bobwhite', genotype effect.

scholarly journals Bioaccessibility of curcumin encapsulated in yeast cells and yeast cell wall particles

2020 ◽  
Vol 309 ◽  
pp. 125700 ◽  
Author(s):  
Stephen Young ◽  
Rewa Rai ◽  
Nitin Nitin
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cells ◽  
Yeast Cell Wall

scholarly journals Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism

2020 ◽  
Vol 31 (10) ◽  
pp. 1069-1084 ◽  
Author(s):  
Heidi M. Blank ◽  
Ophelia Papoulas ◽  
Nairita Maitra ◽  
Riddhiman Garge ◽  
Brian K. Kennedy ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Lipid Metabolism ◽  
Cell Division ◽  
Yeast Cell ◽  
Budding Yeast ◽  
Yeast Cells ◽  
Dynamic Changes ◽  
Coordinate Control ◽  
Cycle Dependent ◽  
Budding Yeast Cell Cycle

In several systems, including budding yeast, cell cycle-dependent changes in the transcriptome are well studied. In contrast, few studies queried the proteome during cell division. There is also little information about dynamic changes in metabolites and lipids in the cell cycle. Here, the authors present such information for dividing yeast cells.

scholarly journals Culturable yeasts in meltwaters draining from two glaciers in the Italian Alps

2005 ◽  
Vol 40 ◽  
pp. 119-122 ◽  
Author(s):  
Pietro Buzzini ◽  
Benedetta Turchetti ◽  
Guglielmina Diolaiuti ◽  
Carlo D’Agata ◽  
Alessandro Martini
Keyword(s):  
Cell Number ◽  
Yeast Cells ◽  
Basidiomycetous Yeasts ◽  
Italian Alps ◽  
Colony Forming Units ◽  
Glacier Ice ◽  
Laboratory Procedures ◽  
Cryptococcus Spp ◽  
Stream Waters ◽  
Subglacial Environments

AbstractThe meltwaters draining from two glaciers in the Italian Alps contain metabolically active yeasts isolable by culture-based laboratory procedures. The average number of culturable yeast cells in the meltwaters was 10–20 colony-forming units (CFU) L–1, whereas supraglacial stream waters originating from overlying glacier ice contained <1 CFUL–1. Yeast cell number increased as the suspended-sediment content of the water samples increased. Basidiomycetous yeasts represent >80% of isolated strains (Cryptococcus spp. and Rhodotorula spp. were 33.3% and 17.8% of total strains, respectively). Culturable yeasts were psychrotolerant, predominantly obligate aerobes and able to degrade organic macromolecules (e.g. starch, esters, lipids, proteins). To the authors’ knowledge, this is the first study to report the presence of culturable yeasts in meltwaters originating from glaciers. On the basis of these results, it is reasonable to suppose that the viable yeasts observed in meltwaters derived predominantly from the subglacial zone and that they originated from the subglacial microbial community. Their metabolic abilities could contribute to the microbial activity occurring in subglacial environments.

Exogenous polyamines enhance somatic embryogenesis and Agrobacterium tumefaciens-mediated transformation efficiency in sugarcane (Saccharum spp. hybrid)

2019 ◽  
Vol 56 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Dorairaj Sathish ◽  
Jeevaraj Theboral ◽  
Venkatachalam Vasudevan ◽  
Gadamchetty Pavan ◽  
Chandrasekaran Ajithan ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Agrobacterium Tumefaciens ◽  
Transformation Efficiency ◽  
Saccharum Spp

Cell de-energization prevents plasmid transformation of yeast Saccharomyces cerevisiae: evidence for the requirement of ATP

2010 ◽  
Vol 5 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Larisa Chaustova ◽  
Valė Miliukienė ◽  
Aurelijus Zimkus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transformation Efficiency ◽  
Energy Requirement ◽  
Yeast Cells ◽  
Sodium Arsenate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Plasmid Transformation ◽  
Substrate Level Phosphorylation ◽  
Intracellular Atp ◽  
Substrate Level

AbstractThe dependence of the yeast Saccharomyces cerevisiae transformation on energy requirement was studied. The inhibitory effect of sodium arsenate, used for the depletion of the intracellular ATP pool, was determined. Incubation of the yeast cells in 5 mM sodium arsenate diminished ATP accumulation by 50% and the transformation efficiency decreased by 65%. To discriminate between ATP produced by substrate level phosphorylation and oxidative phosphorylation, the inhibitory analysis of a mutant with defective mitochondria was performed. Sodium fluoride (10–50 mM), as inhibitor of glycolysis, elicited a concentration-dependent decrease in intracellular ATP levels in both parental and mutant cells. The equal transformation efficiency of the mitochondrial mutant and parental strain, in addition to experiments with oligomycin, demonstrated the independence of plasmid transformation on mitochondrial ATP synthesis. This is consistent with our hypothesis that yeast transformation efficiency is associated with ATP produced by substrate level phosphorylation.

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