scholarly journals Prolines in Transit Peptides Are Crucial for Efficient Preprotein Translocation into Chloroplasts

2017 ◽  
Vol 176 (1) ◽  
pp. 663-677 ◽  
Author(s):  
Dong Wook Lee ◽  
Yun-Joo Yoo ◽  
Md. Abdur Razzak ◽  
Inhwan Hwang
Keyword(s):  
Transit Peptides ◽  
Preprotein Translocation ◽  
In Transit

scholarly journals The essential yeast protein MIM44 (encoded by MPI1) is involved in an early step of preprotein translocation across the mitochondrial inner membrane.

1993 ◽  
Vol 13 (12) ◽  
pp. 7364-7371 ◽  
Author(s):  
J Blom ◽  
M Kübrich ◽  
J Rassow ◽  
W Voos ◽  
P J Dekker ◽  
...  
Keyword(s):  
Protein Import ◽  
Early Stage ◽  
Proteolytic Processing ◽  
Early Step ◽  
Direct Role ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Preprotein Translocation ◽  
In Transit

The essential yeast gene MPI1 encodes a mitochondrial membrane protein that is possibly involved in protein import into the organelle (A. C. Maarse, J. Blom, L. A. Grivell, and M. Meijer, EMBO J. 11:3619-3628, 1992). For this report, we determined the submitochondrial location of the MPI1 gene product and investigated whether it plays a direct role in the translocation of preproteins. By fractionation of mitochondria, the mature protein of 44 kDa was localized to the mitochondrial inner membrane and therefore termed MIM44. Import of the precursor of MIM44 required a membrane potential across the inner membrane and involved proteolytic processing of the precursor. A preprotein in transit across the mitochondrial membranes was cross-linked to MIM44, whereas preproteins arrested on the mitochondrial surface or fully imported proteins were not cross-linked. When preproteins were arrested at two distinct stages of translocation across the inner membrane, only preproteins at an early stage of translocation could be cross-linked to MIM44. Moreover, solubilized MIM44 was found to interact with in vitro-synthesized preproteins. We conclude that MIM44 is a component of the mitochondrial inner membrane import machinery and interacts with preproteins in an early step of translocation.

scholarly journals Sequence Motifs in Transit Peptides Act as Independent Functional Units and Can Be Transferred to New Sequence Contexts

2015 ◽  
Vol 169 (1) ◽  
pp. 471-484 ◽  
Author(s):  
Dong Wook Lee ◽  
Seungjin Woo ◽  
Kyoung Rok Geem ◽  
Inhwan Hwang
Keyword(s):  
Sequence Motifs ◽  
Transit Peptides ◽  
Functional Units ◽  
In Transit

scholarly journals Functional Organization of Sequence Motifs in Diverse Transit Peptides of Chloroplast Proteins

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinseung Jeong ◽  
Inhwan Hwang ◽  
Dong Wook Lee
Keyword(s):  
Functional Organization ◽  
Nuclear Genome ◽  
Transit Peptide ◽  
Sequence Motifs ◽  
Chloroplast Proteins ◽  
Transit Peptides ◽  
Sequence Elements ◽  
Arginine Residues ◽  
Targeting Signal ◽  
In Transit

Although the chloroplasts in plants are characterized by an inherent genome, the chloroplast proteome is composed of proteins encoded by not only the chloroplast genome but also the nuclear genome. Nuclear-encoded chloroplast proteins are synthesized on cytosolic ribosomes and post-translationally targeted to the chloroplasts. In the latter process, an N-terminal cleavable transit peptide serves as a targeting signal required for the import of nuclear-encoded chloroplast interior proteins. This import process is mediated via an interaction between the sequence motifs in transit peptides and the components of the TOC/TIC (translocon at the outer/inner envelope of chloroplasts) translocons. Despite a considerable diversity in primary structures, several common features have been identified among transit peptides, including N-terminal moderate hydrophobicity, multiple proline residues dispersed throughout the transit peptide, preferential usage of basic residues over acidic residues, and an absence of N-terminal arginine residues. In this review, we will recapitulate and discuss recent progress in our current understanding of the functional organization of sequence elements commonly present in diverse transit peptides, which are essential for the multi-step import of chloroplast proteins.

The essential yeast protein MIM44 (encoded by MPI1) is involved in an early step of preprotein translocation across the mitochondrial inner membrane

1993 ◽  
Vol 13 (12) ◽  
pp. 7364-7371
Author(s):  
J Blom ◽  
M Kübrich ◽  
J Rassow ◽  
W Voos ◽  
P J Dekker ◽  
...  
Keyword(s):  
Protein Import ◽  
Early Stage ◽  
Proteolytic Processing ◽  
Early Step ◽  
Direct Role ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Preprotein Translocation ◽  
In Transit

The essential yeast gene MPI1 encodes a mitochondrial membrane protein that is possibly involved in protein import into the organelle (A. C. Maarse, J. Blom, L. A. Grivell, and M. Meijer, EMBO J. 11:3619-3628, 1992). For this report, we determined the submitochondrial location of the MPI1 gene product and investigated whether it plays a direct role in the translocation of preproteins. By fractionation of mitochondria, the mature protein of 44 kDa was localized to the mitochondrial inner membrane and therefore termed MIM44. Import of the precursor of MIM44 required a membrane potential across the inner membrane and involved proteolytic processing of the precursor. A preprotein in transit across the mitochondrial membranes was cross-linked to MIM44, whereas preproteins arrested on the mitochondrial surface or fully imported proteins were not cross-linked. When preproteins were arrested at two distinct stages of translocation across the inner membrane, only preproteins at an early stage of translocation could be cross-linked to MIM44. Moreover, solubilized MIM44 was found to interact with in vitro-synthesized preproteins. We conclude that MIM44 is a component of the mitochondrial inner membrane import machinery and interacts with preproteins in an early step of translocation.

Intercontinental and transcontinental mail transit times from Europe to Berkeley, 1892 to 1941

1986 ◽  
Vol 13 (2) ◽  
pp. 141-154 ◽  
Author(s):  
RUDOLF SCHMID
Keyword(s):  
Transit Times ◽  
In Transit

SUMMARY Mail transit times from Germany to Berkeley, California, are computed for issues of the current awareness journals Botanisches Centralblatt (1880–1945) and the interdisciplinary Naturae Novitates (1879–1944). Issues of the former for 1892 to 1902 averaged 29.3 days (31.2 days if abnormal times are included) in transit from Kassel to Berkeley, with many issues (92) requiring only 20 to 25 days for intercontinental and transcontinental transit. Mail transit of Naturae Novitates from Berlin to Berkeley averaged 40.7 days (42 if abnormal times are included) per issue for 1903 to 1916 and 44 days (51.1 days) per issue for 1922 to 1941 (cumulatively averaging 42 days, or 45.9 days for abnormal times), with some issues in 1906 requiring only 11-12 days for intercontinental and transcontinental transit. A smaller sampling for Nature for 1923 and 1930 gave averages of, respectively, 21.5 and 22.4 days, with a minimum of 14 days in both years. These times are consistent with known transatlantic and transcontinental, ship and rail, mail transit times for these periods, as tabulated from various sources. For perspective, early intercontinental and transcontinental air transit times and pre-1892 intercontinental ship transit times are also tabulated.

Pablo Neruda's Transnational Modernist Networks: Colombo-Madrid-London-Buenos Aires (1927–1933)

2017 ◽  
Vol 12 (2) ◽  
pp. 198-225
Author(s):  
Patricia Novillo-Corvalán
Keyword(s):  
Chamber Music ◽  
Latin American ◽  
Buenos Aires ◽  
Cultural Institutions ◽  
Complex Composition ◽  
British Colonial ◽  
The One ◽  
Composition Process ◽  
In Transit ◽  
Standing Question

This article positions Pablo Neruda's poetry collection Residence on Earth I (written between 1925–1931 and published in 1933) as a ‘text in transit’ that allows us to trace the development of transnational modernist networks through the text's protracted physical journey from British colonial Ceylon (now Sri Lanka) to Madrid, and from José Ortega y Gasset's Revista de Occidente (The Western Review) to T. S. Eliot's The Criterion. By mapping the text's diasporic movement, I seek to reinterpret its complex composition process as part of an anti-imperialist commitment that proposes a form of aesthetic solidarity with artistic modernism in Ceylon, on the one hand, and as a vehicle through which to interrogate the reception and categorisation of Latin American writers and their cultural institutions in a British periodical such as The Criterion, on the other. I conclude with an examination of Neruda's idiosyncratic Spanish translation of Joyce's Chamber Music, which was published in the Buenos Aires little magazine Poesía in 1933, positing that this translation exercise takes to further lengths his decolonising views by giving new momentum to the long-standing question of Hiberno-Latin American relations.

Summary of the SciTech 2020 Technical Panel on In Situ/In Transit Computational Environments for Visualization and Data Analytics

2021 ◽  
Author(s):  
Earl P. Duque ◽  
Steve M. Legensky ◽  
Brad J. Whitlock ◽  
David H. Rogers ◽  
Andrew C. Bauer ◽  
...  
Keyword(s):  
Data Analytics ◽  
In Transit
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