scholarly journals Singlet Oxygen-Induced Membrane Disruption and Serpin-Protease Balance in Vacuolar-Driven Cell Death

2016 ◽  
Vol 171 (3) ◽  
pp. 1616-1625 ◽  
Author(s):  
Eugene Koh ◽  
Raanan Carmieli ◽  
Avishai Mor ◽  
Robert Fluhr
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Membrane Disruption ◽  
Induced Membrane

scholarly journals Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

2021 ◽  
Vol 22 (11) ◽  
pp. 5548
Author(s):  
Yan Li ◽  
Tianyu Tang ◽  
Haejune Lee ◽  
Kiwon Song
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Proliferative Effect ◽  
Huh7 Cells ◽  
Anti Cancer ◽  
Cold Atmospheric Pressure Plasma ◽  
Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

scholarly journals Quantification of C60-induced membrane disruption using a quartz crystal microbalance

RSC Advances ◽  
2018 ◽  
Vol 8 (18) ◽  
pp. 9841-9849 ◽  
Author(s):  
Yuxuan Zeng ◽  
Qi Wang ◽  
Qiu Zhang ◽  
Wei Jiang
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Electrostatic Force ◽  
Membrane Disruption ◽  
Induced Membrane

Fullerene C60 NPs adhere on lipid membrane due to electrostatic force and cause membrane disruption.

Execution of programmed cell death by singlet oxygen generated inside the chloroplasts of Arabidopsis thaliana

PROTOPLASMA ◽  
2020 ◽  
Vol 257 (3) ◽  
pp. 841-851 ◽  
Author(s):  
Vivek Ambastha ◽  
Garima Chauhan ◽  
Budhi Sagar Tiwari ◽  
Baishnab C Tripathy
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Singlet Oxygen

scholarly journals Implication of the oep16-1 Mutation in a flu-Independent, Singlet Oxygen-Regulated Cell Death Pathway in Arabidopsis thaliana

2010 ◽  
Vol 52 (1) ◽  
pp. 84-95 ◽  
Author(s):  
Iga Samol ◽  
Frank Buhr ◽  
Armin Springer ◽  
Stephan Pollmann ◽  
Abder Lahroussi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Cell Death Pathway ◽  
Regulated Cell Death

The active role of Ca2+ ions in Aβ-mediated membrane damage

2018 ◽  
Vol 54 (29) ◽  
pp. 3629-3631 ◽  
Author(s):  
Michele F. M. Sciacca ◽  
Irene Monaco ◽  
Carmelo La Rosa ◽  
Danilo Milardi
Keyword(s):  
Calcium Ions ◽  
Membrane Damage ◽  
Active Role ◽  
Membrane Disruption ◽  
Induced Membrane ◽  
Membrane Poration

Calcium ions inhibits Aβ induced membrane poration by small-sized oligomers but significantly foster fiber-dependent membrane disruption.

scholarly journals Effect of sarcolemmal rupture on myocardial electrical impedance during oxygen deprivation

2005 ◽  
Vol 288 (3) ◽  
pp. H1396-H1403 ◽  
Author(s):  
Antonio Rodriguez-Sinovas ◽  
David García-Dorado ◽  
Pilar Pina ◽  
Marisol Ruiz-Meana ◽  
Jordi Soler-Soler
Keyword(s):  
Cell Death ◽  
Time Course ◽  
Electrical Impedance ◽  
Oxygen Deprivation ◽  
Membrane Disruption ◽  
Protective Effects ◽  
Tissue Impedance ◽  
Membrane Rupture ◽  
Ldh Release ◽  
The Times

Plasma membrane disruption is a characteristic feature of cell death induced by hypoxia or ischemia. Here, we investigated whether analysis of tissue electrical impedance allows detection of ongoing cell membrane rupture and necrotic cell death in hypoxic or ischemic myocardium. Twenty-eight isolated rat hearts were submitted to 5 h of ischemia ( n = 8) or hypoxia ( n = 20). Myocardial electrical impedance and lactate dehydrogenase (LDH) release were monitored. The time course of hypoxia-induced cell death was modified by altering pH (pH 7.4 or 6.4, 5 h) or by adding 3 or 10 mM glycine. Ischemia and hypoxia induced an increase in electrical impedance, followed by a plateau, and later a reduction. During hypoxia, LDH release started after a prolonged lapse of time (80.00 ± 8.37 min at pH 7.4 and 122.50 ± 11.82 min at pH 6.4). The onset of LDH release was followed by the onset of the late reduction in electrical impedance, and both were delayed by acidic pH ( P < 0.05) and by glycine ( P < 0.05). The times of onset of LDH release and of late electrical changes were significantly correlated ( r = 0.752, P < 0.001). In separate experiments, induction of sarcolemmal rupture with Triton X-100 ( n = 6) mimicked the late effects of ischemia or hypoxia on tissue impedance. The protective effects of glycine and acidosis on membrane disruption were confirmed (propidium iodide) in energy-deprived HL-1 cardiomyocytes. These results describe for the first time a late fall in electrical impedance in myocardium submitted to prolonged oxygen deprivation and demonstrate that this fall allows detection of ongoing cell necrosis.

scholarly journals Green light-induced apoptosis in cancer cells by a tetrapyridyl ruthenium prodrug offering two trans coordination sites

2016 ◽  
Vol 7 (8) ◽  
pp. 4922-4929 ◽  
Author(s):  
V. H. S. van Rixel ◽  
B. Siewert ◽  
S. L. Hopkins ◽  
S. H. C. Askes ◽  
A. Busemann ◽  
...  
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Cancer Cells ◽  
Green Light ◽  
Oxygen Generation ◽  
Light Cell ◽  
Singlet Oxygen Generation ◽  
Coordination Sites ◽  
Induced Apoptosis ◽  
Apoptosis In Cancer Cells

In this work, two new photopharmacological ruthenium prodrugs are described that can be activated by green light. Cell death occurs via apoptosis; it is not a consequence of singlet oxygen generation, but of light-induced photosubstitution reactions.

scholarly journals Molecular basis of glycoalkaloid induced membrane disruption

1995 ◽  
Vol 1240 (2) ◽  
pp. 216-228 ◽  
Author(s):  
Erik A.J. Keukens ◽  
Truus de Vrije ◽  
Claudia van den Boom ◽  
Pieter de Waard ◽  
Henk H. Plasman ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Membrane Disruption ◽  
Induced Membrane
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