scholarly journals Quantification of C60-induced membrane disruption using a quartz crystal microbalance

RSC Advances ◽  
2018 ◽  
Vol 8 (18) ◽  
pp. 9841-9849 ◽  
Author(s):  
Yuxuan Zeng ◽  
Qi Wang ◽  
Qiu Zhang ◽  
Wei Jiang
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Electrostatic Force ◽  
Membrane Disruption ◽  
Induced Membrane

Fullerene C60 NPs adhere on lipid membrane due to electrostatic force and cause membrane disruption.

A quartz crystal microbalance-type odor sensor using PVC-blended lipid membrane

1995 ◽  
Vol 44 (3) ◽  
pp. 739-742 ◽  
Author(s):  
G. Matsuno ◽  
D. Yamazaki ◽  
E. Ogita ◽  
K. Mikuriya ◽  
T. Ueda
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane

scholarly journals Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

2019 ◽  
Author(s):  
Jugal Suthar ◽  
Edward Parsons ◽  
Bart Hoogenboom ◽  
Gareth Williams ◽  
Stefan Guldin
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Acoustic Analysis ◽  
Limit Of Detection ◽  
Culture Media ◽  
Transmembrane Protein ◽  
Protein Profile ◽  
Size Exclusion ◽  
Label Free

Exosomes are endocytic lipid-membrane bound bodies with potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterisation approaches has led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalised with anti-CD63 antibodies formed a direct immunoassay towards CD63-positive exosomes, exhibiting a limit-of-detection of 1.7x10^8 and 1.1x10^8 exosome sized particles (ESPs) ml^-1 for frequency and dissipation response respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in diagnostic assays.

Permeability of Model Stratum Corneum Lipid Membrane Measured Using Quartz Crystal Microbalance

Langmuir ◽  
2009 ◽  
Vol 25 (10) ◽  
pp. 5762-5766 ◽  
Author(s):  
Daeyeon Lee ◽  
J. Nathan Ashcraft ◽  
Eric Verploegen ◽  
Eugene Pashkovski ◽  
David A. Weitz
Keyword(s):  
Stratum Corneum ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Stratum Corneum Lipid

scholarly journals Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

2019 ◽  
Author(s):  
Jugal Suthar ◽  
Edward Parsons ◽  
Bart Hoogenboom ◽  
Gareth Williams ◽  
Stefan Guldin
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Acoustic Analysis ◽  
Limit Of Detection ◽  
Culture Media ◽  
Transmembrane Protein ◽  
Protein Profile ◽  
Size Exclusion ◽  
Label Free

Exosomes are endocytic lipid-membrane bound bodies with potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterisation approaches has led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalised with anti-CD63 antibodies formed a direct immunoassay towards CD63-positive exosomes, exhibiting a limit-of-detection of 1.7x10^8 and 1.1x10^8 exosome sized particles (ESPs) ml^-1 for frequency and dissipation response respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in diagnostic assays.

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