scholarly journals Transcription Factor ATAF1 in Arabidopsis Promotes Senescence by Direct Regulation of Key Chloroplast Maintenance and Senescence Transcriptional Cascades

2015 ◽  
Vol 168 (3) ◽  
pp. 1122-1139 ◽  
Author(s):  
Prashanth Garapati ◽  
Gang-Ping Xue ◽  
Sergi Munné-Bosch ◽  
Salma Balazadeh
Keyword(s):  
Transcription Factor ◽  
Direct Regulation ◽  
Transcriptional Cascades

scholarly journals Direct Regulation by the Vibrio cholerae Regulator ToxT To Modulate Colonization and Anticolonization Pilus Expression

2009 ◽  
Vol 77 (4) ◽  
pp. 1383-1388 ◽  
Author(s):  
Ansel Hsiao ◽  
Xiao Xu ◽  
Biao Kan ◽  
Rahul V. Kulkarni ◽  
Jun Zhu
Keyword(s):  
Transcription Factor ◽  
Vibrio Cholerae ◽  
Transcriptional Repression ◽  
Type Iv Pili ◽  
Present Evidence ◽  
Type Iv ◽  
Transcriptional Regulatory ◽  
Environmental Reservoir ◽  
Target Promoters ◽  
Direct Regulation

ABSTRACT The pathogen Vibrio cholerae uses a large number of coordinated transcriptional regulatory events to transition from its environmental reservoir to the host and establish itself at its preferred colonization site at the host intestinal mucosa. The key regulator in this process is the AraC/XylS family transcription factor, ToxT, which plays critical roles in pathogenesis, including the regulation of two type IV pili, the anticolonization factor mannose-sensitive hemagglutinin and the toxin-coregulated pilus. Previously, it was thought ToxT required dimerization in order to effect transcriptional regulation at its cognate promoters. Here, we present evidence that ToxT directly represses transcription of the msh operon by binding to three promoters within this operon and that dimerization may not be required for transcriptional repression of target promoters by ToxT, suggesting that this regulator uses different mechanisms to modulate the transcriptional repertoire of V. cholerae.

scholarly journals Identification of the Novel Player δEF1 in Estrogen Transcriptional Cascades

1999 ◽  
Vol 19 (5) ◽  
pp. 3600-3606 ◽  
Author(s):  
Elaine M. Chamberlain ◽  
Michel M. Sanders
Keyword(s):  
Estrogen Receptor ◽  
Transcription Factor ◽  
Binding Site ◽  
Target Gene ◽  
Transcriptional Level ◽  
Receptor Complex ◽  
The Novel ◽  
Regulatory Cascade ◽  
Transcriptional Cascades

ABSTRACT Although many genes are regulated by estrogen, very few have been shown to directly bind the estrogen receptor complex. Therefore, transcriptional cascades probably occur in which the estrogen receptor directly binds to a target gene that encodes another transcription factor that subsequently regulates additional genes. Through the use of a differential display assay, a transcription factor has been identified that may be involved in estrogen transcriptional cascades. This report demonstrates that transcription factor δEF1 is induced eightfold by estrogen in the chick oviduct. Furthermore, the regulation by estrogen occurs at the transcriptional level and is likely to be a direct effect of the estrogen receptor complex, as it does not require concomitant protein synthesis. A putative binding site was identified in the 5′-flanking region of the chick ovalbumin gene identifying it as a possible target gene for regulation by δEF1. Characterization of this binding site revealed that δEF1 binds to and regulates the chick ovalbumin gene. Thus, a novel regulatory cascade that is triggered by estrogen has been defined.

scholarly journals Tomato SlBES1.8 influences leaf morphogenesis by mediating gibberellin metabolism and signaling

2021 ◽  
Author(s):  
Deding Su ◽  
Wei Xiang ◽  
Qin Liang ◽  
Ling Wen ◽  
Yuan Shi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Regulatory Mechanism ◽  
Tomato Leaf ◽  
Negative Regulator ◽  
The Other ◽  
Leaf Morphogenesis ◽  
Gibberellin Metabolism ◽  
Expression Studies ◽  
Ga Biosynthesis ◽  
Direct Regulation

Leaf morphogenetic activity determines its shape diversity. However, our knowledge to the regulatory mechanism in maintaining leaf morphogenetic capacity is still limited. In tomato, gibberellin (GA) negatively regulates leaf complexity by shortening the morphogenetic window. We here reported a tomato BRI1-EMS-SUPPRESSOR 1 (BES1) transcription factor, SlBES1.8, that promoted the simplification of leaf pattern in a similar manner as GA functions. Enhanced level of SlBES1.8 dramatically decreased the sensibility of tomato to GA whereas increased the sensibility to the GA biosynthesis inhibitor, PAC. In line with the phenotypic observation, the endogenous bioactive GA contents were increased in OE-SlBES1.8 lines, which certainly promoted the degradation of the GA signaling negative regulator, SlDELLA. Moreover, transcriptomic analysis uncovered a set of overlapping genomic targets of SlBES1.8 and GA, and most of them were regulated in the same way. Expression studies showed the repression of SlBES1.8 to the transcriptions of two GA deactivated genes, SlGA2ox2 and SlGA2ox6, and one GA receptor, SlGID1b-1. Further experiments confirmed the direct regulation of SlBES1.8 to their promoters. On the other hand, SlDELLA physically interacted with SlBES1.8 and further inhibited its transcriptional regulation activity by abolishing SlBES1.8-DNA binding. Conclusively, by mediating GA deactivation and signaling, SlBES1.8 greatly influenced tomato leaf morphogenesis.

scholarly journals MicroRNA-466 inhibits tumor growth and bone metastasis in prostate cancer by direct regulation of osteogenic transcription factor RUNX2

2017 ◽  
Vol 8 (1) ◽  
pp. e2572-e2572 ◽  
Author(s):  
Melissa Colden ◽  
Altaf A Dar ◽  
Sharanjot Saini ◽  
Priya V Dahiya ◽  
Varahram Shahryari ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Transcription Factor ◽  
Bone Metastasis ◽  
Tumor Growth ◽  
Direct Regulation

scholarly journals The MAPK Slt2/Mpk1 plays a role in iron homeostasis through direct regulation of the transcription factor Aft1

2021 ◽  
Vol 1868 (5) ◽  
pp. 118974
Author(s):  
Nuria Pujol-Carrion ◽  
Mónica Pavón-Vergés ◽  
Javier Arroyo ◽  
Maria Angeles de la Torre-Ruiz
Keyword(s):  
Transcription Factor ◽  
Iron Homeostasis ◽  
Direct Regulation

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

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