scholarly journals Molecular Interactions between the Specialist HerbivoreManduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata. II. Accumulation of Plant mRNAs in Response to Insect-Derived Cues

2001 ◽  
Vol 125 (2) ◽  
pp. 701-710 ◽  
Author(s):  
Ursula Schittko ◽  
Dieter Hermsmeier ◽  
Ian T. Baldwin
Keyword(s):  
Molecular Interactions ◽  
Natural Host ◽  
Nicotiana Attenuata

scholarly journals Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata. VII. Changes in the Plant's Proteome

2006 ◽  
Vol 142 (4) ◽  
pp. 1621-1641 ◽  
Author(s):  
Ashok P. Giri ◽  
Hendrik Wünsche ◽  
Sirsha Mitra ◽  
Jorge A. Zavala ◽  
Alexander Muck ◽  
...  
Keyword(s):  
Manduca Sexta ◽  
Molecular Interactions ◽  
Natural Host ◽  
Nicotiana Attenuata ◽  
Specialist Herbivore

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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