scholarly journals Biosynthesis of l-Ascorbic Acid and Conversion of Carbons 1 and 2 of l-Ascorbic Acid to Oxalic Acid Occurs within Individual Calcium Oxalate Crystal Idioblasts

2001 ◽  
Vol 125 (2) ◽  
pp. 634-640 ◽  
Author(s):  
Todd A. Kostman ◽  
Nathan M. Tarlyn ◽  
Frank A. Loewus ◽  
Vincent R. Franceschi
Keyword(s):  
Ascorbic Acid ◽  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Calcium Oxalate Crystal

Research note:Autoradiography utilising labelled ascorbic acid reveals biochemical and morphological details in diverse calcium oxalate crystal-forming species

2007 ◽  
Vol 34 (4) ◽  
pp. 339 ◽  
Author(s):  
Todd A. Kostman ◽  
Nathan M. Tarlyn ◽  
Vincent R. Franceschi
Keyword(s):  
Ascorbic Acid ◽  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Pistia Stratiotes ◽  
Calcium Oxalate Crystals ◽  
Calcium Oxalate Crystal ◽  
Water Lily ◽  
Oxalate Crystals ◽  
Crystal Sand ◽  
Acid Precursor

Many plant species accumulate calcium oxalate crystals in specialised cells called crystal idioblasts. In one species of crystal-forming plants (Pistia stratiotes L.; forming raphide crystals), it has been shown that ascorbic acid is the primary precursor of oxalic acid. The question remains if this is true of other calcium oxalate crystal-forming plants. One way of answering the above question is by examining ascorbic acid as the oxalic acid precursor in diverse species with a variety of crystal types. In this study we tested ascorbic acid as the primary precursor of oxalic acid in four different species, each forming one of the four, thus far, unexamined crystal types: water hyacinth, styloid (and raphide); tomato, crystal sand; winged-bean, prismatic; water lily, astrosclereids with surface prismatic crystals. Pulse–chase feeding of 1-[14C]-ascorbic acid followed by resin embedding, microautoradiography and light microscopy were employed to examine incorporation of label into calcium oxalate crystals. For the species and crystal types studied, ascorbic acid is the primary precursor of oxalic acid and further, oxalic acid is added to crystals in patterns that correlate with the age and type of crystal involved.

L-Ascorbic acid and L-galactose are sources for oxalic acid and calcium oxalate in Pistia stratiotes

2000 ◽  
Vol 53 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Sarah E. Keates ◽  
Nathan M. Tarlyn ◽  
Frank A. Loewus ◽  
Vincent R. Franceschi
Keyword(s):  
Ascorbic Acid ◽  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Pistia Stratiotes

An SEM study of raphide crystal initials in the leaves of vitis (grape)

1995 ◽  
Vol 53 ◽  
pp. 984-985
Author(s):  
H. J. Arnott ◽  
M. A. Webb ◽  
L. E. Lopez
Keyword(s):  
Calcium Oxalate ◽  
Water Soluble ◽  
Calcium Oxalate Crystals ◽  
Calcium Oxalate Crystal ◽  
Oxalate Crystals ◽  
Large Numbers ◽  
Sem Study ◽  
The Matrix ◽  
Crystal Cells ◽  
Crystal Idioblast

Many papers have been published on the structure of calcium oxalate crystals in plants, however, few deal with the early development of crystals. Large numbers of idioblastic calcium oxalate crystal cells are found in the leaves of Vitis mustangensis, V. labrusca and V. vulpina. A crystal idioblast, or raphide cell, will produce 150-300 needle-like calcium oxalate crystals within a central vacuole. Each raphide crystal is autonomous, having been produced in a separate membrane-defined crystal chamber; the idioblast''s crystal complement is collectively embedded in a water soluble glycoprotein matrix which fills the vacuole. The crystals are twins, each having a pointed and a bidentate end (Fig 1); when mature they are about 0.5-1.2 μn in diameter and 30-70 μm in length. Crystal bundles, i.e., crystals and their matrix, can be isolated from leaves using 100% ETOH. If the bundles are treated with H2O the matrix surrounding the crystals rapidly disperses.

scholarly journals THE METABOLISM OF ASCORBIC ACID-1-C14 AND OXALIC ACID-C14 IN THE RAT

1955 ◽  
Vol 216 (2) ◽  
pp. 539-548
Author(s):  
Constance O'H. Curtin ◽  
C.G. King
Keyword(s):  
Ascorbic Acid ◽  
Oxalic Acid

Ascorbic Acid Stability in Ground Asparagus Samples and in Oxalic Acid Extracts

1995 ◽  
Vol 60 (6) ◽  
pp. 1282-1283
Author(s):  
MARÍA J. ESTEVE ◽  
ROSAURA FARRÉ ◽  
ANA FRÍGOLA
Keyword(s):  
Ascorbic Acid ◽  
Oxalic Acid ◽  
Acid Stability

Oxalic acid and calcium oxalate produced by Leucostoma cincta and L. persoonii in culture and in peach bark tissues

1987 ◽  
Vol 65 (9) ◽  
pp. 1952-1956 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Crystal Morphology ◽  
Culture Media ◽  
Positive Staining ◽  
Acid Solutions ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

Oxalic acid and crystals of calcium oxalate were produced during growth of Leucostoma cincta and L. persoonii on potato dextrose agar and in peach bark tissues. The identification of calcium oxalate was based on solubility characteristics, the results of KMnO4 titration, positive staining with silver nitrate – dithiooxamide, and crystal morphology as observed with light and scanning electron microscopes. Oxalic acid was detected by gas chromatography. This is the first report of oxalic acid production by both Leucostoma species causing peach canker. Calcium oxalate crystals observed on or near hyphae in culture were similar to crystals in artificially inoculated peach bark tissues. Addition of oxalic acid solutions alone to inner bark tissues caused maceration and necrosis. These results indicate a role for oxalic acid in the early stages of pathogenesis by Leucostoma spp. Tetragonal (bipyramidal) and prismatic calcium oxalate crystals formed on bark wounds treated with oxalic acid solutions were similar to those observed in infected tissues and in culture media amended with oxalic acid.

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