scholarly journals Control of Cl− Efflux in Chara corallina by Cytosolic pH, Free Ca2+, and Phosphorylation Indicates a Role of Plasma Membrane Anion Channels in Cytosolic pH Regulation

1998 ◽  
Vol 118 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Eva Johannes ◽  
Alan Crofts ◽  
Dale Sanders
Keyword(s):  
Plasma Membrane ◽  
Ph Regulation ◽  
Chara Corallina ◽  
Anion Channels ◽  
Cytosolic Ph

Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH-regulatory role of plasma membrane V-ATPases.

1995 ◽  
Vol 198 (8) ◽  
pp. 1711-1715 ◽  
Author(s):  
T A Heming ◽  
D L Traber ◽  
F Hinder ◽  
A Bidani
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Initial Rate ◽  
Cell Types ◽  
Peritoneal Macrophages ◽  
Bafilomycin A1 ◽  
Atpase Inhibitor ◽  
Cytosolic Ph ◽  
Phi Regulation

The role of plasma membrane V-ATPase activity in the regulation of cytosolic pH (pHi) was determined for resident alveolar and peritoneal macrophages (m theta) from sheep. Cytosolic pH was measured using 2',7'-biscarboxyethyl-5,6-carboxyfluorescein (BCECF). The baseline pHi of both cell types was sensitive to the specific V-ATPase inhibitor bafilomycin A1. Bafilomycin A1 caused a significant (approximately 0.2 pH units) and rapid (within seconds) decline in baseline pHi. Further, bafilomycin A1 slowed the initial rate of pHi recovery (dpHi/dt) from intracellular acid loads. Amiloride had no effects on baseline pHi, but reduced dpHi/dt (acid-loaded pHi nadir < 6.8) by approximately 35%. Recovery of pHi was abolished by co-treatment of m theta with bafilomycin A1 and amiloride. These data indicate that plasma membrane V-ATPase activity is a major determinant of pHi regulation in resident alveolar and peritoneal m theta from sheep. Sheep m theta also appear to possess a Na+/H+ exchanger. However, Na+/H+ exchange either is inactive or can be effectively masked by V-ATPase-mediated H+ extrusion at physiological pHi values.

scholarly journals Distinct pH regulation of slow and rapid anion channels at the plasma membrane of Arabidopsis thaliana hypocotyl cells

2005 ◽  
Vol 56 (417) ◽  
pp. 1897-1903 ◽  
Author(s):  
Jean Colcombet ◽  
Françoise Lelièvre ◽  
Sébastien Thomine ◽  
Hélène Barbier-Brygoo ◽  
Jean-Marie Frachisse
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Ph Regulation ◽  
Anion Channels

scholarly journals Cytosolic pH regulation in mouse macrophages. Proton extrusion by plasma-membrane-localized H+-ATPase

1992 ◽  
Vol 281 (1) ◽  
pp. 245-250 ◽  
Author(s):  
H Tapper ◽  
R Sundler
Keyword(s):  
Plasma Membrane ◽  
Lactic Acid ◽  
Steady State ◽  
Ph Regulation ◽  
Lysosomal Hydrolases ◽  
Proton Extrusion ◽  
Cytosolic Ph ◽  
Low Concentrations ◽  
Atpase Inhibitors ◽  
Mouse Macrophages

Recent evidence indicates that H+ extrusion in macrophages is in part accomplished by a H(+)-ATPase of vacuolar type. The presence and plasma-membrane localization of such a mechanism in adherent resident macrophages was verified by inhibition of H+ extrusion, monitored by changes in both cytosolic pH (pHi) and extracellular pH, with low concentrations of the H(+)-ATPase inhibitors N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole. The H(+)-ATPase was operative at physiological pHi levels, thus contributing to maintenance of steady-state pHi. It was further shown to be sensitive to the plasma-membrane potential, with hyperpolarization being strongly inhibitory. In addition, H+ extrusion mediated by the H(+)-ATPase and the generation and release of lactic acid caused acidification of the pericellular space and could enable secreted lysosomal hydrolases to act extracellularly.

Cytosolic pH regulation in perfused rat liver: role of intracellular bicarbonate production

1998 ◽  
Vol 1425 (1) ◽  
pp. 224-234 ◽  
Author(s):  
Giovanni Vidal ◽  
Thierry Durand ◽  
Paul Canioni ◽  
Jean-Louis Gallis
Keyword(s):  
Rat Liver ◽  
Ph Regulation ◽  
Perfused Rat Liver ◽  
Cytosolic Ph

Role of cytoplasmic inorganic phosphate in light-induced activation of H+-pumps in the plasma membrane and tonoplast of Chara corallina

Planta ◽  
1992 ◽  
Vol 186 (3) ◽  
Author(s):  
Kazuhiko Takeshige ◽  
Fumiyuki Mitsumori ◽  
Masashi Tazawa ◽  
Tetsuro Mimura
Keyword(s):  
Plasma Membrane ◽  
Inorganic Phosphate ◽  
Chara Corallina

Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics

2013 ◽  
Vol 454 (2) ◽  
pp. 227-237 ◽  
Author(s):  
Thomas Walther ◽  
Narjes Mtimet ◽  
Ceren Alkim ◽  
Amélie Vax ◽  
Marie-Odile Loret ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Metabolic Response ◽  
Adenine Nucleotides ◽  
Carbon Sources ◽  
Amp Deaminase ◽  
Cytosolic Ph ◽  
Fermentative Metabolism ◽  
Encoding Gene

In Saccharomyces cerevisiae, synthesis of T6P (trehalose 6-phosphate) is essential for growth on most fermentable carbon sources. In the present study, the metabolic response to glucose was analysed in mutants with different capacities to accumulate T6P. A mutant carrying a deletion in the T6P synthase encoding gene, TPS1, which had no measurable T6P, exhibited impaired ethanol production, showed diminished plasma membrane H+-ATPase activation, and became rapidly depleted of nearly all adenine nucleotides which were irreversibly converted into inosine. Deletion of the AMP deaminase encoding gene, AMD1, in the tps1 strain prevented inosine formation, but did not rescue energy balance or growth on glucose. Neither the 90%-reduced T6P content observed in a tps1 mutant expressing the Tps1 protein from Yarrowia lipolytica, nor the hyperaccumulation of T6P in the tps2 mutant had significant effects on fermentation rates, growth on fermentable carbon sources or plasma membrane H+-ATPase activation. However, intracellular metabolite dynamics and pH homoeostasis were strongly affected by changes in T6P concentrations. Hyperaccumulation of T6P in the tps2 mutant caused an increase in cytosolic pH and strongly reduced growth rates on non-fermentable carbon sources, emphasizing the crucial role of the trehalose pathway in the regulation of respiratory and fermentative metabolism.

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