Role of HCO−3Ions in Cytosolic pH Regulation in Rat Mast Cells: Evidence for a New Na+-Independent, HCO−3-Dependent Alkalinizing Mechanism

1998 ◽  
Vol 253 (2) ◽  
pp. 320-324 ◽  
Author(s):  
Natalia Vilariño ◽  
Mercedes R. Vieytes ◽  
Juan M. Vieites ◽  
Luis M. Botana
Keyword(s):  
Mast Cells ◽  
Ph Regulation ◽  
Cytosolic Ph ◽  
Rat Mast Cells

Soluble proteins as modulators of the exocytotic reaction of permeabilised rat mast cells

1989 ◽  
Vol 94 (3) ◽  
pp. 585-591
Author(s):  
A. Koffer ◽  
B.D. Gomperts
Keyword(s):  
Plasma Membrane ◽  
Mast Cells ◽  
Soluble Proteins ◽  
The Other ◽  
Guanine Nucleotide ◽  
Cytoplasmic Proteins ◽  
Streptolysin O ◽  
Two Factors ◽  
Rat Mast Cells

This study addresses the question of the role of cytoplasmic proteins in exocytosis from permeabilised rat mast cells. We have used two different methods of cell permeabilisation (ATP4- and streptolysin O) to regulate the size of the plasma membrane lesions, and thus to dictate the rate and extent of efflux of the cytosolic proteins, and compared the secretory response of the two preparations. We report evidence for the existence of two factors present in the cytosol, which affect the exocytotic mechanism in opposing manners. One of these is required for the maintenance of cell responsiveness; it is retained for more than 120 min by ATP4- -permeabilised cells but lost within 60 min from cells permeabilised by streptolysin O. The other factor, which leaks immediately from cells treated from streptolysin O, but only gradually from cells treated with ATP4-, has the effect of suppressing the affinity for both Ca2+ and guanine nucleotide in the exocytotic reaction.

Cytosolic pH regulation in perfused rat liver: role of intracellular bicarbonate production

1998 ◽  
Vol 1425 (1) ◽  
pp. 224-234 ◽  
Author(s):  
Giovanni Vidal ◽  
Thierry Durand ◽  
Paul Canioni ◽  
Jean-Louis Gallis
Keyword(s):  
Rat Liver ◽  
Ph Regulation ◽  
Perfused Rat Liver ◽  
Cytosolic Ph
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