scholarly journals The Role of Gibberellin, Abscisic Acid, and Sucrose in the Regulation of Potato Tuber Formation in Vitro

1998 ◽  
Vol 117 (2) ◽  
pp. 575-584 ◽  
Author(s):  
Xin Xu ◽  
André A.M. van Lammeren ◽  
Evert Vermeer ◽  
Dick Vreugdenhil
Keyword(s):  
Abscisic Acid ◽  
Potato Tuber ◽  
Tuber Formation

scholarly journals Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

2021 ◽  
Author(s):  
Antonio Calabuig-Serna ◽  
Carolina Camacho-Fernández ◽  
Ricardo Mir ◽  
Rosa Porcel ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Growth Regulators ◽  
Solanum Melongena ◽  
Microspore Embryogenesis ◽  
Anther Wall ◽  
Naphthaleneacetic Acid ◽  
Shed Light ◽  
Embryogenic Response

AbstractIn eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from being efficient. In this work we aimed to shed light on the factors involved in the successful in vitro development of eggplant haploid embryos by evaluating the role of growth regulators (GRs) in this process. We analyzed the endogenous levels of different GRs, including auxins, cytokinins and gibberelins, as well as salicylic, jasmonic and abscisic acid, in microspores and microspore-derived embryos at different culture stages. We also analyzed the same GR profiles in leaf and anther wall tissues of different eggplant backgrounds. Finally, we assessed the application of different GR combinations to the culture medium. Our results showed that in eggplant there are no genotype-specific endogenous GR profiles that can be associated to a high embryogenic response. Instead, the embryogenic response seems related to different GR accumulation patterns during in vitro culture. The changes observed in the endogenous levels of salicylic and abscisic acid were not related to the embryo transition. There were, however, changes in the levels of indole acetic acid and dihydrozeatin. The best GR combination to promote callus production was 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.5 mg/L 6-benzylaminopurine (BAP). A 20% reduction of NAA and BAP reduced embryo production but produced structures more anatomically similar to embryos. These results shed light on the role of GRs during the development of microspore-derived embryos in eggplant microspore cultures.

The role of polyamines in potato tuber formation

1992 ◽  
Vol 28 (2) ◽  
pp. 81-86 ◽  
Author(s):  
Calixto M. Protacio ◽  
Hector E. Flores
Keyword(s):  
Potato Tuber ◽  
Tuber Formation

scholarly journals Effect of exogenous abscisic acid on accumulation of raffinose family oligosaccharides and galactosyl cyclitols in tiny vetch seeds (Vicia hirsuta [L.] S.F. Gray)

2011 ◽  
Vol 73 (4) ◽  
pp. 277-283 ◽  
Author(s):  
Lesław B. Lahuta ◽  
Ryszard J. Górecki ◽  
Ewa Gojło ◽  
Marcin Horbowicz
Keyword(s):  
Abscisic Acid ◽  
Seed Development ◽  
Acid Concentration ◽  
Raffinose Family Oligosaccharides ◽  
High Concentration ◽  
Low Concentration ◽  
Vicia Hirsuta ◽  
Galactosyl Cyclitols

The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (<em>Vicia hirsuta</em> [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant and in seed maturing in vitro. In early stages of <em>V. hirsuta</em> seed development, the ABA activated the biosynthesis of galactinol, although the level of arisen galactinol quickly declined. In the later stages of <em>V. hirsuta</em> seed development ABA had stimulatory effect of RFOs and Gal-C biosynthesis. Influence of ABA on biosynthesis of a-galactosides in <em>Vicia hirsuta</em> seed seems to be dependent on abscisic acid concentration. Low concentration of ABA had stimulatory effect on a-galactosides biosynthesis, but high concentration of ABA inhibited the process.

Use of the growth retardant tetcyclacis for potato tuber formation in vitro

1994 ◽  
Vol 14 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Dick Vreugdenhil ◽  
Petra Bindels ◽  
Poula Reinhoud ◽  
Jozef Klocek ◽  
Theo Hendriks
Keyword(s):  
Potato Tuber ◽  
Growth Retardant ◽  
Tuber Formation

The Role of Abscisic Acid in Acclimation of Plants Cultivated in vitro to ex vitro Conditions

2003 ◽  
Vol 46 (4) ◽  
pp. 535-541 ◽  
Author(s):  
M. Hronkova ◽  
H. Zahradnickova ◽  
M. Simkova ◽  
P. Simek ◽  
A. Heydova
Keyword(s):  
Abscisic Acid ◽  
Ex Vitro

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

An integrated screening method for evaluating the pulmonary toxicity of inhaled particulates: Role of microscopic techniques in assessing pulmonary macrophage clearance functions

1990 ◽  
Vol 48 (3) ◽  
pp. 826-827
Author(s):  
David B. Warheit ◽  
Lena Achinko ◽  
Mark A. Hartsky
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pulmonary Toxicity ◽  
Screening Method ◽  
Pulmonary Response ◽  
Inhaled Particles ◽  
Cytochemical Analysis ◽  
Pulmonary Macrophages ◽  
Integrated Screening

There is a great need for the development of a rapid and reliable bioassay to evaluate the pulmonary toxicity of inhaled particles. A number of methods have been proposed, including lung clearance studies, bronchoalveolar lavage analysis, and in vitro cytotoxicity tests. These methods are often limited in scope inasmuch as they measure only one dimension of the pulmonary response to inhaled, instilled or incubated dusts. Accordingly, a comprehensive approach to lung toxicity studies has been developed.To validate the method, rats were exposed for 6 hours or 3 days to various concentrations of either aerosolized alpha quartz silica (Si) or carbonyl iron (CI) particles. Cells and fluids from groups of sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL). Alkaline phosphatase, LDH and protein values were measured in BAL fluids at several time points postexposure. Cells were counted and evaluated for viability, as well as differential and cytochemical analysis. In addition, pulmonary macrophages (PM) were cultured and studied for morphology, chemotaxis, and phagocytosis by scanning electron microscopy.

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