Use of the growth retardant tetcyclacis for potato tuber formation in vitro

1994 ◽  
Vol 14 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Dick Vreugdenhil ◽  
Petra Bindels ◽  
Poula Reinhoud ◽  
Jozef Klocek ◽  
Theo Hendriks
Keyword(s):  
Potato Tuber ◽  
Growth Retardant ◽  
Tuber Formation

scholarly journals The Role of Gibberellin, Abscisic Acid, and Sucrose in the Regulation of Potato Tuber Formation in Vitro

1998 ◽  
Vol 117 (2) ◽  
pp. 575-584 ◽  
Author(s):  
Xin Xu ◽  
André A.M. van Lammeren ◽  
Evert Vermeer ◽  
Dick Vreugdenhil
Keyword(s):  
Abscisic Acid ◽  
Potato Tuber ◽  
Tuber Formation

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

scholarly journals Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 29-35
Author(s):  
Usman Kris Joko Suharjo ◽  
Hasanudin Hasanudin ◽  
Tunjung Pamekas ◽  
Hesti Pujiwati ◽  
Alyi Vanturini
Keyword(s):  
Poor Quality ◽  
Tuber Formation ◽  
Potato Tubers ◽  
Seed Sources ◽  
Randomized Design ◽  
Plant Culture ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The  research  design  used  was  Completely  Randomized  Design  (CRD)  which  consist  of  18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and  Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

scholarly journals Effect of CCC and BA on the Formation of Potato Tuber in vitro.

1997 ◽  
Vol 66 (4) ◽  
pp. 663-668 ◽  
Author(s):  
Tomohide YAMAMOTO ◽  
Kazuo NAKATA
Keyword(s):  
Potato Tuber

Activation of Protein Synthesis in Aging Potato Tuber Slices

1971 ◽  
Vol 26 (10) ◽  
pp. 1064-1067 ◽  
Author(s):  
Günter Kahl
Keyword(s):  
Protein Synthesis ◽  
Potato Tuber ◽  
Actinomycin D ◽  
Leucine Incorporation ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Fresh Potato ◽  
Polyuridylic Acid ◽  
Storage Organs

Whereas ribosome preparations of freshly sliced potato disks do not show appreciable activity in an in-vitro amino acid incorporation system, aging of the tissue leads to a greatly enhanced incorporation activity which reaches its maximum 24 hours after slicing. If ribosomes from freshly excised disks are provided with polyuridylic acid, their activity in the incorporation of phenylalanine is increased about 8 fold.Moreover, an RNA-fraction can be dissociated by EDTA from ribosomes of aged potato tuber slices, which sediments at 15 —18S, has a base composition different from that of 16S — rRNA, 5S-and 4S —RNA, and is not present on ribosomes of fresh slices. Its appearance is inhibited by actinomycin D and therefore most probably dependent on transcription. This compound, purified from sucrose gradients, enhances in vitro leucine incorporation into peptide material by ribosomes of fresh potato slices.The possibility is discussed that this fraction-among other factors-is responsible for the enhanced protein synthesis after slicing plant storage organs, and is indicative of a general derepression phenomenon in these tissues.

Tuber-Specific Expression of Two Gibberellin Oxidase Transgenes from Arabidopsis Regulates over Wide Ranges the Potato Tuber Formation

2019 ◽  
Vol 66 (6) ◽  
pp. 984-991 ◽  
Author(s):  
O. O. Kolachevskaya ◽  
S. N. Lomin ◽  
M. Kojima ◽  
I. A. Getman ◽  
L. I. Sergeeva ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Tuber Formation ◽  
Specific Expression
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