scholarly journals Adaptation of Active Proton Pumping and Plasmalemma ATPase Activity of Corn Roots to Low Root Medium pH

1998 ◽  
Vol 117 (1) ◽  
pp. 311-319 ◽  
Author(s):  
Feng Yan ◽  
Robert Feuerle ◽  
Stefanie Schäffer ◽  
Helge Fortmeier ◽  
Sven Schubert
Keyword(s):  
Atpase Activity ◽  
Proton Pumping ◽  
Medium Ph ◽  
Root Medium ◽  
Corn Roots

Effect of antimicrobial peptides on ATPase activity and proton pumping in plasma membrane vesicles obtained from mycobacteria

Peptides ◽  
2012 ◽  
Vol 36 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Paola Santos ◽  
Aldemar Gordillo ◽  
Luis Osses ◽  
Luz-Mary Salazar ◽  
Carlos-Yesid Soto
Keyword(s):  
Plasma Membrane ◽  
Antimicrobial Peptides ◽  
Atpase Activity ◽  
Membrane Vesicles ◽  
Proton Pumping ◽  
Plasma Membrane Vesicles

Inhibition of proton pumping in corn roots is associated with increased phosphorylation of membrane proteins

1983 ◽  
Vol 31 (2-3) ◽  
pp. 215-221 ◽  
Author(s):  
Graziano Zocchi ◽  
Sharon A. Rogers ◽  
John B. Hanson
Keyword(s):  
Membrane Proteins ◽  
Proton Pumping ◽  
Corn Roots

Poly(L‐aspartate) inhibits the proton pumping of tonoplast H+‐atpase from corn roots

1996 ◽  
Vol 19 (5) ◽  
pp. 743-754
Author(s):  
Shu‐I Tu ◽  
Deidre Patterson ◽  
Marlise Franke‐Snyder ◽  
David Brauer
Keyword(s):  
Proton Pumping ◽  
Corn Roots

scholarly journals Response of Zonal and Ivy Geraniums to Root Medium pH

HortScience ◽  
2006 ◽  
Vol 41 (5) ◽  
pp. 1351-1355 ◽  
Author(s):  
Pauline H. Andrews ◽  
P. Allen Hammer
Keyword(s):  
Dry Weight ◽  
Hydrated Lime ◽  
Pine Bark ◽  
Medium Ph ◽  
Greenhouse Production ◽  
Sphagnum Peat ◽  
Root Medium ◽  
Root Media

Three cultivars each of zonal geranium (Pelargonium ×hortorum `Candy Lavender', `Fireball', and `Patriot Red') and ivy geraniums (Pelargonium pelatum `Global Deep Lilac', `Global Salmon Rose', and `Global Soft Pink') were grown in root media with pHs varying from 4.3 to 7.8. In Expt. 1, a mixture of sphagnum peat, fine perlite, and fine pine bark was modified with limestone and hydrated lime at the following rates: 0, 1.2, 3.0, 4.7, and 11.9 kg·m–3 limestone; 11.9 limestone plus 5.9 hydrated lime; 11.9 limestone plus 8.3 hydrated lime; and 11.9 kg·m–3 limestone plus 10.7 kg·m–3 hydrated lime to give the various root medium pH treatments. Plants were grown for 11 weeks in glass greenhouses. In Expt. 2, plants were grown in two commercial soilless mixes with one being modified with the addition of 0 kg·m–3 limestone, 6.0 kg·m–3 limestone plus 0.6 kg·m–3 hydrated lime, and 6.0 kg·m–3 limestone plus 2.4 kg·m–3 hydrated lime. In both experiments, greatest dry weight was recorded in zonal and ivy geraniums plants grown at root medium pHs above 6.4. This study showed a root medium pH of 6.4 to 6.5 should be recommended for the greenhouse production of both zonal and ivy geraniums.

scholarly journals Loss of the apical V-ATPase a-subunit VHA-6 prevents acidification of the intestinal lumen during a rhythmic behavior in C. elegans

2009 ◽  
Vol 297 (5) ◽  
pp. C1071-C1081 ◽  
Author(s):  
Erik Allman ◽  
David Johnson ◽  
Keith Nehrke
Keyword(s):  
Epithelial Cells ◽  
Atpase Activity ◽  
Genetic Model ◽  
Intestinal Epithelial Cells ◽  
Proton Pumping ◽  
Intestinal Lumen ◽  
Short Time Scale ◽  
Intestinal Epithelial ◽  
Ph Homeostasis ◽  
A Subunit

In Caenorhabditis elegans, oscillations of intestinal pH contribute to the rhythmic defecation behavior, but the acid-base transport mechanisms that facilitate proton movement are not well understood. Here, we demonstrate that VHA-6, an intestine-specific a-subunit of the H+-K+-ATPase complex (V-ATPase), resides in the apical membrane of the intestinal epithelial cells and is required for luminal acidification. Disruption of the vha-6 gene led to early developmental arrest; the arrest phenotype could be complemented by expression of a fluorescently labeled vha-6 transgene. To study the contribution of vha-6 to pH homeostasis in larval worms, we used a partial reduction of function through postembryonic single-generation RNA interference. We demonstrate that the inability to fully acidify the intestinal lumen coincides with a defect in pH recovery of the intestinal epithelial cells, suggesting that VHA-6 is essential for proton pumping following defecation. Moreover, intestinal dipeptide accumulation and fat storage are compromised by the loss of VHA-6, suggesting that luminal acidification promotes nutrient uptake in worms, as well as in mammals. Since acidified intracellular vesicles and autofluorescent storage granules are indistinguishable between the vha-6 mutant and controls, it is likely that the nutrient-restricted phenotype is due to a loss of plasma membrane V-ATPase activity specifically. These data establish a simple genetic model for proton pump-driven acidification. Since defecation occurs at 45-s intervals in worms, this model represents an opportunity to study acute regulation of V-ATPase activity on a short time scale and may be useful in the study of alternative treatments for acid-peptic disorders.

scholarly journals Response of Zonal and Ivy Geraniums to Root Medium pH

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 766E-767
Author(s):  
Pauline H. Kaufmann* ◽  
P. Allen Hammer
Keyword(s):  
Dry Weight ◽  
Hydrated Lime ◽  
Research Data ◽  
Medium Ph ◽  
Root Medium ◽  
Wide Range ◽  
The Media ◽  
Garden Plant ◽  
Published Research

In 2002 the USDA reported potted geraniums accounted for $150 million in wholesale value, more than any other bedding or garden plant surveyed. Despite the importance of the geranium in floriculture production, little published research data is available pertaining to the media pH requirements of zonal and ivy geraniums. Current recommendations suggest zonal geraniums be grown at pH 5.7-6.6 and ivy geraniums at pH 5.0-6.2. The wide range in root medium pH recommendations for both zonal and ivy geraniums and the lack of research data prompted this research. Also, the basis for recommending a lower medium pH for ivy geraniums could not be found in published literature. The research objectives were to investigate the effect of medium pH on plant growth and to determine more precise recommendations for both species. The growth of 3 cultivars each of zonal and ivy geraniums growing in 8 medium pH treatments were evaluated. Limestone and hydrated lime were incorporated at increasing rates into a 1:1:1 peat, perlite and bark mix to achieve a medium pH ranging from pH 4.0-7.5. Plants were harvested at weeks 3, 6, and 11 and plant dry weight and media pH were determined. Leaf luminance, chroma and hue were evaluated at week 10. Plant dry weight was greatest at pH 6.55 or higher for both zonal and ivy geraniums at week 11. Leaves of plants grown at pH 6.55 or higher had significantly lower luminance and chroma and greater hue in all cultivars, corresponding to leaves that were darker, less vivid, and deeper green in color. This study shows a root medium pH greater than pH 6.5 results in greatest plant dry weight accumulation and quality of leaf color for both zonal and ivy geraniums. This study also shows ivy geraniums can be grown at the same media pH as zonal geraniums.

scholarly journals Evidence for a conserved 95-120 kDa subunit associated with and essential for activity of V-ATPases.

1992 ◽  
Vol 172 (1) ◽  
pp. 105-112 ◽  
Author(s):  
M F Manolson ◽  
D Proteau ◽  
E W Jones
Keyword(s):  
Membrane Protein ◽  
Atpase Activity ◽  
Proton Pump ◽  
Integral Membrane Protein ◽  
Proton Pumping ◽  
Cell Fractionation ◽  
Expression Library ◽  
Suppressor Factor ◽  
Reading Frame ◽  
Cell Extracts

Vacuoles purified from Saccharomyces cerevisiae bearing the vph1-1 mutation had no detectable bafilomycin-sensitive ATPase activity or ATP-dependent proton pumping. Furthermore, the vacuolar H(+)-ATPase (V-ATPase) nucleotide binding subunits were no longer associated with vacuolar membranes yet were present at wild-type levels in yeast whole-cell extracts. The VPH1 gene was cloned by screening a lambda gt11 expression library with antibodies directed against a 95 kDa vacuolar integral membrane protein and independently cloned by complementation of the vph1-1 mutation. Deletion disruption of the VPH1 gene revealed that the VPH1 gene is required for vacuolar H(+)-ATPase assembly and vacuolar acidification but is not essential for cell viability or for targeting and maturation of vacuolar proteases. VPH1 encodes a predicted polypeptide of 840 amino acid residues (95.6 kDa) with putative membrane-spanning regions. Cell fractionation and immunodetection demonstrate that Vph1p is a vacuolar integral membrane protein that co-purifies with V-ATPase activity. Vph1p has 42% identity to the 116 kDa polypeptide of the rat clathrin-coated vesicles/synaptic vesicle proton pump, 42% identity to the TJ6 mouse immune suppressor factor, 42% identity to the Caenorhabditis elegans proton pump homologue and 54% identity to the predicted polypeptide encoded by the yeast gene STV1 (Similar To VPH1, identified as an open reading frame next to the BUB2 gene.

scholarly journals Irrigation Requirements, Root-medium pH, and Nutrient Concentrations of Easter Lilies Grown in Five Peat-based Media with and without an Evaporation Barrier

1994 ◽  
Vol 119 (6) ◽  
pp. 1151-1156 ◽  
Author(s):  
William R. Argo ◽  
John A. Biernbaum
Keyword(s):  
Root Zone ◽  
Water Soluble ◽  
Nutrient Concentrations ◽  
Average Amount ◽  
Applied Water ◽  
Medium Ph ◽  
Root Medium ◽  
Root Media ◽  
Soluble Fertilizer ◽  
Water Holding

Subirrigated Easter lilies were grown in five commercially formulated root media using one water-soluble fertilizer applied independently to each medium based on water-holding capacity and water loss. The number of irrigations ranged from 12 to 20 and the amount of applied water ranged from 5.3 to 6.8 liters for the uncovered media treatments. When the root-medium surface was covered with an evaporation barrier, the average amount of applied water was reduced by 35% compared to the uncovered media. The largest effect on root media pH was between uncovered and covered media due to the reduced amount of water applied. Similar macronutrient concentrations were measured in the five media during the experiment with few exceptions. The greatest differences in nutrient concentrations were found within the pots. The top 2.5 cm (top layer) contained nutrient concentrations up to 10 times higher than those measured in the remaining root medium (root zone) of the same pot. Covering the root-medium surface with an evaporation barrier reduced the stratification of fertilizer salts. Root-zone soluble salt concentrations of plants in the covered pots were similar to those of uncovered plants even though 36% less fertilizer was applied to the covered plants.

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