Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during Infection

1998 ◽  
Vol 10 (7) ◽  
pp. 1107 ◽  
Author(s):  
Manfred Heinlein ◽  
Hal S. Padgett ◽  
J. Scott Gens ◽  
Barbara G. Pickard ◽  
Steven J. Casper ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement ◽  
Changing Patterns

scholarly journals Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during Infection

1998 ◽  
Vol 10 (7) ◽  
pp. 1107-1120 ◽  
Author(s):  
Manfred Heinlein ◽  
Hal S. Padgett ◽  
J. Scott Gens ◽  
Barbara G. Pickard ◽  
Steven J. Casper ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement ◽  
Changing Patterns

scholarly journals Dysfunctionality of a tobacco mosaic virus movement protein mutant mimicking threonine 104 phosphorylation

2003 ◽  
Vol 84 (3) ◽  
pp. 727-732 ◽  
Author(s):  
E. M. Karger ◽  
O. Yu. Frolova ◽  
N. V. Fedorova ◽  
L. A. Baratova ◽  
T. V. Ovchinnikova ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Cell Movement ◽  
Specific Protein ◽  
Virus Movement ◽  
Wild Type ◽  
Molecular Masses

Replication of tobacco mosaic virus (TMV) is connected with endoplasmic reticulum (ER)-associated membranes at early stages of infection. This study reports that TMV movement protein (MP)-specific protein kinases (PKs) associated with the ER of tobacco were capable of phosphorylating Thr104 in TMV MP. The MP-specific PKs with apparent molecular masses of about 45–50 kDa and 38 kDa were revealed by gel PK assays. Two types of mutations were introduced in TMV MP gene of wild-type TMV U1 genome to substitute Thr104 by neutral Ala or by negatively charged Asp. Mutation of Thr104 to Ala did not affect the size of necrotic lesions induced by the mutant virus in Nicotiana tabacum Xanthi nc. plants. Conversely, mutation of Thr to Asp mimicking Thr104 phosphorylation strongly inhibited cell-to-cell movement. The possible role of Thr104 phosphorylation in TMV MP function is discussed.

scholarly journals Challenging the Role of Microtubules in Tobacco Mosaic Virus Movement by Drug Treatments Is Disputable

2006 ◽  
Vol 80 (13) ◽  
pp. 6712-6715 ◽  
Author(s):  
Mark Seemanpillai ◽  
Rabab Elamawi ◽  
Christophe Ritzenthaler ◽  
Manfred Heinlein
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Viral Rna ◽  
Virus Movement ◽  
Direct Role ◽  
Microtubule Inhibitors ◽  
Microtubule Polymerization ◽  
Drug Treatments

ABSTRACT The movement protein (MP) of Tobacco mosaic virus interacts with microtubules during infection. Although this interaction is correlated with the function of MP in the cell-to-cell transport of viral RNA, a direct role of microtubules in the movement process was recently challenged by studies involving the treatment of plants with inhibitors of microtubule polymerization. Here, we report evidence suggesting that such treatments may not efficiently disrupt all microtubules. Thus, results obtained from studies using microtubule inhibitors may have to remain open to interpretation with regard to the involvement of microtubules in viral RNA trafficking.

scholarly journals Tobacco mosaic virus Movement Protein Enhances the Spread of RNA Silencing

2008 ◽  
Vol 4 (4) ◽  
pp. e1000038 ◽  
Author(s):  
Hannes Vogler ◽  
Myoung-Ok Kwon ◽  
Vy Dang ◽  
Adrian Sambade ◽  
Monika Fasler ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Movement Protein ◽  
Virus Movement

scholarly journals Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids.

1992 ◽  
Vol 4 (4) ◽  
pp. 397-411 ◽  
Author(s):  
V Citovsky ◽  
M L Wong ◽  
A L Shaw ◽  
B V Prasad ◽  
P Zambryski
Keyword(s):  
Nucleic Acids ◽  
Protein Binding ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement

scholarly journals Tobacco Mosaic Virus Movement Protein Interacts with Green Fluorescent Protein-Tagged Microtubule End-Binding Protein 1

2008 ◽  
Vol 147 (2) ◽  
pp. 611-623 ◽  
Author(s):  
Katrin Brandner ◽  
Adrian Sambade ◽  
Emmanuel Boutant ◽  
Pascal Didier ◽  
Yves Mély ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Fluorescent Protein ◽  
Movement Protein ◽  
Binding Protein ◽  
Virus Movement ◽  
Green Fluorescent
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