scholarly journals Formation of Di-Isodityrosine and Loss of Isodityrosine in the Cell Walls of Tomato Cell-Suspension Cultures Treated with Fungal Elicitors or H2O2

1997 ◽  
Vol 115 (1) ◽  
pp. 87-92 ◽  
Author(s):  
J. D. Brady ◽  
S. C. Fry
Keyword(s):  
Cell Suspension ◽  
Cell Walls ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Fungal Elicitors

Significant Enhancement of Triterpenoids Production by Phomopsis Elicitors in Cell Suspension Cultures of Betula Platyphylla Suk.

2011 ◽  
Vol 335-336 ◽  
pp. 105-110 ◽  
Author(s):  
Qiao Li Zhai ◽  
Ya Guang Zhan ◽  
Dao Qi Xu ◽  
Xiao Dong Wang ◽  
Gui Zhi Fan
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Endophytic Fungi ◽  
Molecular Level ◽  
Mrna Level ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Betula Platyphylla ◽  
Fungal Elicitors ◽  
Betula Platyphylla Suk

58 fungal elicitors prepared from the endophytic fungi isolated from inner bark of B. platyphylla Suk. were examined to determine their effects on the growth and triterpenoid production in suspension cultures of Betula platyphylla Suk. cells. The results showed that different fungal elicitors could cause diversely stimulating effects. Among the fungal elicitors tested, BE58 stimulated the highest triterpenoids yield with 15.90 mg•L-1 and 183.72% higher than the control. The experiment also affirmed from mRNA level that triterpenoid was indeed accumulated in suspension culture of birch cells treated by BE58 fungal elicitor. Meantime BE58 was identified as Phomopsis from the morphological and molecular level.

Proteomic analysis of changes in the extracellular matrix of Arabidopsis cell suspension cultures induced by fungal elicitors

PROTEOMICS ◽  
2003 ◽  
Vol 3 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Bongani K. Ndimba ◽  
Stephen Chivasa ◽  
John M. Hamilton ◽  
William J. Simon ◽  
Antoni R. Slabas
Keyword(s):  
Extracellular Matrix ◽  
Cell Suspension ◽  
Proteomic Analysis ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Fungal Elicitors

scholarly journals Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures

2015 ◽  
Vol 57 (4) ◽  
pp. 357-372 ◽  
Author(s):  
Hugo Mélida ◽  
Asier Largo-Gosens ◽  
Esther Novo-Uzal ◽  
Rogelio Santiago ◽  
Federico Pomar ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Walls ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Deficient Cell ◽  
Maize Cell

Factors Affecting Protoplast Isolation and the Regeneration of Shoot-Like Structures From Protoplast-Derived Callus of Sugarcane (Saccharum spp Hybrids)

1992 ◽  
Vol 40 (6) ◽  
pp. 863 ◽  
Author(s):  
PWJ Taylor ◽  
HL Ko ◽  
SW Adkins
Keyword(s):  
Cell Suspension ◽  
Cell Walls ◽  
Cell Suspension Cultures ◽  
Protoplast Isolation ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Factors Affecting ◽  
Nodular Callus ◽  
Homogeneous Cell ◽  
High Yields

High yields of protoplasts were isolated from non-regenerable, homogeneous cell suspension cultures of sugarcane, compared with regenerable, heterogeneous cell suspension cultures after incubation in an enzyme composition containing Cellulase RS, Pectinase, Macerozyme and Driselase. Higher yields of protoplasts were released from heterogeneous cell suspension cultures after the addition of 10 mg L-1 silver nitrate to the culture medium; however, ethylene production was not involved in protoplast isolation. Use of 0-05-2% Pectolyase Y23 pectinase rather than other pectinases resulted in higher yields of protoplasts from heterogeneous cell suspension cultures. These results suggest that there are differences in the cell walls between cells from heterogeneous and homogeneous cell suspension cultures which affect the isolation of protoplasts. Protoplasts isolated from heterogeneous cell suspension cultures failed to develop beyond the cell division stage. Protoplasts isolated from homogeneous cell suspension cultures and cultured in agarose droplets bathed in modified 8p medium, reformed cell walls, divided and developed into microcallus. Microcallus transferred to solid modified MS medium containing 1 mg -1 .2,4-D developed into callus. Protoplast-derived callus from one cultivar formed compact nodular callus when subcultured onto the same medium containing 1% activated charcoal. Incubation of this callus on MS medium containing BAP at 0.5 mg L-1, then a combination of BAP and fluridone each at 0.5 mg L-1, resulted in the regeneration of small, chlorophyll-containing shoot-like structures. As yet no intact plants have developed from these shoot-like structures.

A β-glucosidase associated with cell walls from cell suspension cultures of carrot

1996 ◽  
Vol 43 (6) ◽  
pp. 1157-1161 ◽  
Author(s):  
Haruyoshi Konno ◽  
Yoshiki Yamasaki ◽  
Kenji Katoh
Keyword(s):  
Cell Suspension ◽  
Cell Walls ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Ultrastructural and biochemical effects of endopectate lyase on cell walls from cell suspension cultures of bean and rice

1980 ◽  
Vol 58 (8) ◽  
pp. 867-880 ◽  
Author(s):  
Con J. Baker ◽  
James R. Aist ◽  
Durward F. Bateman
Keyword(s):  
Cell Wall ◽  
Cell Suspension ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Middle Lamella ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Wall Integrity ◽  
Wall Material ◽  
Wall Structure

Cell walls prepared from suspension cell cultures of bean and rice in log-phase growth were used to examine the effects of endopectate lyase (PL) on the solubilization of cell wall carbohydrates and concomitant ultrastructural alterations. Cell wall preparations from both plant sources were heterogeneous and contained a range of wall types from primary walls to xylem elements with spiral, secondary wall thickenings. Marked differences in wall thickness and number of wall laminations typified both preparations.Bean cell walls were more susceptible to degradation by PL than were those of rice. Upon treatment of the former with 2.3 × 10−3 units/mL of PL (1 unit released 1 μmol of unsaturated uronide/min at 30 °C from polygalacturonic acid at pH 8.5), 27% of the noncellulosic wall carbohydrate was solubilized in 1 h. This represented 50% of the PL susceptible carbohydrate in the preparation. Only 3% of the noncellulosic carbohydrate was released from rice cell walls in 1 h when treated with 115 × 10−3 units of PL/mL. This accounted for 60% of the PL susceptible wall fractions. Only uronic acid, rhamnose, galactose, and arabinose were solubilized from both preparations by PL.Cell walls in the bean and rice preparations were affected differentially by the PL. Those walls with secondary thickenings did not appear to be degraded, while the distinct fibrillar appearance of both bean and rice walls tended to fade or disappear. The middle lamella tended to dissolve to varying degrees in the presence of PL. Bean walls were more severely degraded than were the rice walls and many exhibited swelling, separation of wall layers, markedly reduced staining intensity, and (or) a granular ultrastructure.This study has demonstrated that as PL acts on susceptible cell walls there are major changes evoked in cell wall structure which suggest that the rhamnogalacturonan fraction of the higher plant cell wall contributes significantly to cell wall integrity. This study also emphasizes the need for cell wall material of proven uniformity for investigations of both cell wall composition and effects of specific polysaccharide degrading enzymes on cell wall integrity. Preliminary studies indicate that tobacco pith may provide more uniform cell walls than do cell suspension cultures.

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