Proteomic analysis of changes in the extracellular matrix of Arabidopsis cell suspension cultures induced by fungal elicitors

PROTEOMICS ◽  
2003 ◽  
Vol 3 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Bongani K. Ndimba ◽  
Stephen Chivasa ◽  
John M. Hamilton ◽  
William J. Simon ◽  
Antoni R. Slabas
Keyword(s):  
Extracellular Matrix ◽  
Cell Suspension ◽  
Proteomic Analysis ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Fungal Elicitors

Significant Enhancement of Triterpenoids Production by Phomopsis Elicitors in Cell Suspension Cultures of Betula Platyphylla Suk.

2011 ◽  
Vol 335-336 ◽  
pp. 105-110 ◽  
Author(s):  
Qiao Li Zhai ◽  
Ya Guang Zhan ◽  
Dao Qi Xu ◽  
Xiao Dong Wang ◽  
Gui Zhi Fan
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Endophytic Fungi ◽  
Molecular Level ◽  
Mrna Level ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Betula Platyphylla ◽  
Fungal Elicitors ◽  
Betula Platyphylla Suk

58 fungal elicitors prepared from the endophytic fungi isolated from inner bark of B. platyphylla Suk. were examined to determine their effects on the growth and triterpenoid production in suspension cultures of Betula platyphylla Suk. cells. The results showed that different fungal elicitors could cause diversely stimulating effects. Among the fungal elicitors tested, BE58 stimulated the highest triterpenoids yield with 15.90 mg•L-1 and 183.72% higher than the control. The experiment also affirmed from mRNA level that triterpenoid was indeed accumulated in suspension culture of birch cells treated by BE58 fungal elicitor. Meantime BE58 was identified as Phomopsis from the morphological and molecular level.

Proteomic analysis of dark response in Arabidopsis cell suspension cultures

2012 ◽  
Vol 169 (17) ◽  
pp. 1690-1697 ◽  
Author(s):  
Yun Wang ◽  
Antoni R. Slabas ◽  
Stephen Chivasa
Keyword(s):  
Cell Suspension ◽  
Proteomic Analysis ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures

Proteomes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 29
Author(s):  
Mamosa G. Ngcala ◽  
Tatenda Goche ◽  
Adrian P. Brown ◽  
Stephen Chivasa ◽  
Rudo Ngara
Keyword(s):  
Extracellular Matrix ◽  
Heat Stress ◽  
Cell Suspension ◽  
Secretory Pathway ◽  
Transcriptional Response ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Extracellular Proteins ◽  
Protein Accumulation ◽  
Heat Response

Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 °C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes. Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (≥2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.

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