scholarly journals Methyl-CpG-Binding Domain Protein MBD7 Is Required for Active DNA Demethylation in Arabidopsis

2015 ◽  
Vol 167 (3) ◽  
pp. 905-914 ◽  
Author(s):  
Chunlei Wang ◽  
Xiaomei Dong ◽  
Dan Jin ◽  
Yusheng Zhao ◽  
Shaojun Xie ◽  
...  
Keyword(s):  
Dna Demethylation ◽  
Binding Domain ◽  
Active Dna Demethylation ◽  
Domain Protein

scholarly journals Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana

PLoS Genetics ◽  
2015 ◽  
Vol 11 (5) ◽  
pp. e1005210 ◽  
Author(s):  
Qi Li ◽  
Xiaokang Wang ◽  
Han Sun ◽  
Jun Zeng ◽  
Zhendong Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Demethylation ◽  
Binding Domain ◽  
Active Dna Demethylation ◽  
Domain Protein

scholarly journals Correction: Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana

PLoS Genetics ◽  
2015 ◽  
Vol 11 (7) ◽  
pp. e1005380 ◽  
Author(s):  
Qi Li ◽  
Xiaokang Wang ◽  
Han Sun ◽  
Jun Zeng ◽  
Zhendong Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Demethylation ◽  
Binding Domain ◽  
Active Dna Demethylation ◽  
Domain Protein

scholarly journals Regulation of Active DNA Demethylation by an α-Crystallin Domain Protein in Arabidopsis

2014 ◽  
Vol 55 (3) ◽  
pp. 361-371 ◽  
Author(s):  
Weiqiang Qian ◽  
Daisuke Miki ◽  
Mingguang Lei ◽  
Xiaohong Zhu ◽  
Huiming Zhang ◽  
...  
Keyword(s):  
Dna Demethylation ◽  
Active Dna Demethylation ◽  
Domain Protein

DNA demethylation induced by the methyl-CpG-binding domain protein MBD3

Gene ◽  
2008 ◽  
Vol 420 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Shelley E. Brown ◽  
Matthew J. Suderman ◽  
Michael Hallett ◽  
Moshe Szyf
Keyword(s):  
Dna Demethylation ◽  
Binding Domain ◽  
Domain Protein

scholarly journals A novel protein complex that regulates active DNA demethylation in Arabidopsis

2020 ◽  
Author(s):  
Pan Liu ◽  
Wen-Feng Nie ◽  
Xiansong Xiong ◽  
Yuhua Wang ◽  
Yuwei Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Protein Complex ◽  
Excision Repair ◽  
Dna Demethylation ◽  
Loss Of Function ◽  
Dna Hypermethylation ◽  
Active Dna Demethylation ◽  
Domain Protein ◽  
Genomic Regions

SUMMARYActive DNA demethylation is critical for altering DNA methylation patterns and regulating gene expression. The 5-methylcytosine DNA glycosylase/lyase ROS1 initiates a base excision repair pathway for active DNA demethylation and is required for the prevention of DNA hypermethylation at thousands of genomic regions in Arabidopsis. How ROS1 is regulated and targeted to specific genomic regions is not well understood. Here, we report the discovery of an Arabidopsis protein complex that contains ROS1, regulates ROS1 gene expression, and likely targets the ROS1 protein to specific genomic regions. ROS1 physically interacts with a WD40 domain protein (RWD40), which in turn interacts with a methyl-DNA binding protein (RMB1) as well as with a zinc finger and homeobox domain protein (RHD1). RMB1 binds to DNA that is methylated in any sequence context, and this binding is necessary for its function in vivo.Loss-of-function mutations in RWD40, RMB1, or RHD1 cause DNA hypermethylation at several tested genomic regions independently of the known ROS1 regulator IDM1. Because the hypermethylated genomic regions include the DNA methylation monitoring sequence in the ROS1 promoter, plants mutated in RWD40, RMB1, or RHD1 show increased ROS1 expression. Importantly, ROS1 binding to the ROS1 promoter requires RWD40, RMB1, and RHD1, suggesting that this complex dictates ROS1 targeting to this locus. Our results demonstrate that ROS1 forms a protein complex with RWD40, RMB1, and RHD1, and that this novel complex regulates active DNA demethylation at several endogenous loci in Arabidopsis.

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