scholarly journals Immunological Detection of Potential Signal-Transduction Proteins Expressed during Wheat Somatic Tissue Culture

1997 ◽  
Vol 113 (3) ◽  
pp. 801-807 ◽  
Author(s):  
A. Nato ◽  
A. Mirshahi ◽  
G. Tichtinsky ◽  
M. Mirshahi ◽  
J. P. Faure ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Tissue Culture ◽  
Somatic Tissue ◽  
Immunological Detection ◽  
Somatic Tissue Culture ◽  
Signal Transduction Proteins ◽  
Potential Signal

RESISTANCE RESPONSES OF PLANTS REGENERATED FROM TOMATO ANTHER AND SOMATIC TISSUE CULTURES TO CLAVIBACTER MICHIGANENSE SUBSP. MICHIGANENSE

1999 ◽  
Vol 47 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Violeta Sotirova ◽  
Lydia Shtereva ◽  
Nedjalka Zagorska ◽  
Boyan Dimitrov ◽  
Nevena Bogatsevska
Keyword(s):  
Tissue Culture ◽  
Anther Culture ◽  
Somaclonal Variation ◽  
Genetic Improvement ◽  
Somatic Tissue ◽  
Tissue Cultures ◽  
Disease Symptoms ◽  
Tomato Plants ◽  
Somaclonal Variants ◽  
Somatic Tissue Culture

The production of gametoclonal and somaclonal variants in tomato is of great importance for the genetic improvement of tomato hybrids and cultivars. The possibility to obtain tomato plants resistant to Clavibacter michiganense subsp. michiganense (Cmm) through anther and tissue culture was investigated in the present study. Regenerants from anther and tissue cultures and their progenies (R1-R3) in the cultivars Roma ms and Bella, lines L. 24–13, and L. 6944, as well as the hybrids Roma ms × UC 82A, Roma ms × L. 31, Roma ms × Bella, and Cristy, were tested for resistance to Cmm. The regenerants differed in their resistance to Cmm. All regenerants from the anther culture of the genotype Roma ms and Roma ms × UC 82A are susceptible to the disease, while those from Bella and Roma ms × L. 31 vary from susceptible to resistant. The highest number of regenerants obtained from somatic tissue culture lacking disease symptoms until the end of the vegetation was observed in the genotypes Cristy and L. 24–13. Variation in regenerant resistance is found in R1, Rb, and R3. The results suggest that the gametoclonal and somaclonal variation may be efficiently applied to obtain tomato plants resistant to Cmm.

scholarly journals Evaluation of Factors Affecting Direct Organogenesis in a Somatic Tissue Culture of Sinningia speciosa (Lodd.) Hiern

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1783
Author(s):  
Aušra Blinstrubienė ◽  
Natalija Burbulis ◽  
Vaida Jonytienė ◽  
Ramunė Masienė
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Somatic Tissue ◽  
Explant Position ◽  
Shoot Number ◽  
Somatic Tissue Culture ◽  
Number Of Shoots

In this study, the influence of genotype, concentration of thidiazuron (TDZ), and explant position on the culture medium in organogenesis in a somatic tissue culture of two gloxinia cultivars was investigated. Isolated explants cultured on the medium containing TDZ formed adventitious shoots directly without an intervening callus phase. Explant regeneration frequency varied depending on the genotype, TDZ concentration, and explant position on the medium. The analysis of variance revealed that cultivar (C), TDZ concentration (T), position of explant on culture medium (P), and the interaction of C × T, C × P, T × P, and C × T × P significantly influenced the frequency of shoot formation. However, the effect of interface C × P and C × T × P on the number of shoots per explant was not significant. “Snowy” leaf explants manifested a significantly higher mean shoot formation frequency (p ˂ 0.01) in comparison with the cultivar “Midnight Purple”. The medium enriched with 3.5 μM TDZ resulted in the highest organogenesis frequency, while the highest shoot number per explant was acquired on medium supplemented with 4.0 μM TDZ. The explants of the tested cultivars cultured on the medium with the adaxial side down showed a significantly higher (p ˂ 0.01) shoot formation frequency in comparison with explants cultured on the medium with the abaxial side, and they showed a higher mean number of shoots per explant. An effective method for in vitro organogenesis of Sinningia speciosa (Lodd.) Hiern without an intervening callus phase was established.

scholarly journals Divergence in Coding Sequence and Expression of Different Functional Categories of Immune Genes between Two Wild Rodent Species

2021 ◽  
Vol 13 (3) ◽  
Author(s):  
Xiuqin Zhong ◽  
Max Lundberg ◽  
Lars Råberg
Keyword(s):  
Signal Transduction ◽  
Transcription Factors ◽  
Immune Function ◽  
Sequence Divergence ◽  
Expression Divergence ◽  
Functional Categories ◽  
Coding Sequence ◽  
Immune Genes ◽  
Genes Encoding ◽  
Signal Transduction Proteins

Abstract Differences in immune function between species could be a result of interspecific divergence in coding sequence and/or expression of immune genes. Here, we investigate how the degree of divergence in coding sequence and expression differs between functional categories of immune genes, and if differences between categories occur independently of other factors (expression level, pleiotropy). To this end, we compared spleen transcriptomes of wild-caught yellow-necked mice and bank voles. Immune genes expressed in the spleen were divided into four categories depending on the function of the encoded protein: pattern recognition receptors (PRR); signal transduction proteins; transcription factors; and cyto- and chemokines and their receptors. Genes encoding PRR and cyto-/chemokines had higher sequence divergence than genes encoding signal transduction proteins and transcription factors, even when controlling for potentially confounding factors. Genes encoding PRR also had higher expression divergence than genes encoding signal transduction proteins and transcription factors. There was a positive correlation between expression divergence and coding sequence divergence, in particular for PRR genes. We propose that this is a result of that divergence in PRR coding sequence leads to divergence in PRR expression through positive feedback of PRR ligand binding on PRR expression. When controlling for sequence divergence, expression divergence of PRR genes did not differ from other categories. Taken together, the results indicate that coding sequence divergence of PRR genes is a major cause of differences in immune function between species.

scholarly journals Drug Screening on Signal Transduction Proteins Via μ-Patterned Surfaces

2012 ◽  
Vol 102 (3) ◽  
pp. 31a
Author(s):  
Peter Lanzerstorfer ◽  
Stefan Sunzenauer ◽  
Mario Brameshuber ◽  
Gerhard J. Schütz ◽  
Shin-Ichiro Takahashi ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Drug Screening ◽  
Patterned Surfaces ◽  
Signal Transduction Proteins

scholarly journals 2P076 Interactions between signal transduction proteins studied by surface forces measurement(01D. Protein: Function,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S171
Author(s):  
Asuka Sakai ◽  
Hitomi Fujiwara ◽  
Masaya Fujita ◽  
Kazue Kurihara
Keyword(s):  
Signal Transduction ◽  
Protein Function ◽  
Surface Forces ◽  
Signal Transduction Proteins

scholarly journals The molecular mechanisms of multidrug resistance of human glioblastomas

2021 ◽  
Vol 67 (1) ◽  
pp. 20-28
Author(s):  
Alexandr Chernov ◽  
Irina Baldueva ◽  
Tatyana Nekhaeva ◽  
Elvira Galimova ◽  
Diana Alaverdian ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Drug Resistance ◽  
Transcription Factors ◽  
Growth Factors ◽  
Multidrug Resistance ◽  
Tumor Suppressor Genes ◽  
Molecular Mechanisms ◽  
Molecular Targets ◽  
Polymorphic Variants ◽  
Signal Transduction Proteins

In review discusses the phenomenon of drug resistance of GB in the context of the expression of ABC family transporter proteins and the processes of proliferation, angiogenesis, recurrence and death. The emphasis is on the identifying for molecular targets among growth factors, receptors, signal transduction proteins, microRNAs, transcription factors, proto-oncogenes, tumor suppressor genes and their polymorphic variants (SNPs) for the development and creation of targeted anticancer drugs.

scholarly journals The Role of Cgrp-Receptor Component Protein (Rcp) in Cgrp-Mediated Signal Transduction

2001 ◽  
Vol 1 ◽  
pp. 12-12
Author(s):  
M. A. Prado ◽  
B. Evans-Bain ◽  
S. L. Santi ◽  
I. M. Dickerson
Keyword(s):  
Signal Transduction ◽  
Cgrp Receptor ◽  
Nih3t3 Cells ◽  
New Class ◽  
Receptor Component ◽  
Calcitonin Gene ◽  
Cellular Signal ◽  
Signal Transduction Proteins ◽  
Component Protein

The calcitonin gene-related peptide (CGRP)-receptor component protein (RCP) is a 17-kDa intracellular peripheral membrane protein required for signal transduction at CGRP receptors. To determine the role of RCP in CGRP-mediated signal transduction, RCP was depleted from NIH3T3 cells using antisense strategy. Loss of RCP protein correlated with loss of cAMP production by CGRP in the antisense cells. In contrast, loss of RCP had no effect on CGRP-mediated binding; therefore RCP is not acting as a chaperone for the CGRP receptor. Instead, RCP is a novel signal transduction molecule that couples the CGRP receptor to the cellular signal transduction machinery. RCP thus represents a prototype for a new class of signal transduction proteins that are required for regulation of G protein-coupled receptors.

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